11 documents found, page 1 of 2
Enhanced neuronal differentiation by dynamic piezoelectric stimulation
Pinho, Tiffany S.; Silva, Deolinda; Ribeiro, Jorge Cibrão; Marote, Ana; Lima, Rui; Batista, Salete J.; Melo, Rita; Ribeiro, Clarisse Marta OliveiraAbstract Electroactive smart materials play an important role for tissue regenerative applications. Poly(vinylidene fluoride) (PVDF) is a specific subtype of piezoelectric electroactive material that generates electrical potential upon mechanical stimulation. This work focuses on the application of piezoelectric PVDF films for neural differentiation. Human neural precursor cells (hNPCs) are cultured on piezoele...
Fractionating stem cells secretome for Parkinson's disease modeling: Is it the ...
Vilaça-Faria, Helena; Marote, Ana; Lages, Inês; Ribeiro, Clarisse; Mendes-Pinheiro, Bárbara; Domingues, Ana Verónica; Campos, Jonas; Lanceros-Méndez, S.Human mesenchymal stem cells (hMSCs) secretome has been have been at the forefront of a new wave of possible therapeutic strategies for central nervous system neurodegenerative disorders, as Parkinson's disease (PD). While within its protein fraction, several promising proteins were already identified with therapeutic properties on PD, the potential of hMSCs-secretome vesicular fraction remains to be elucidated...
Impact of aging on the 6-OHDA-induced rat model of Parkinson’s disease
Barata-Antunes, Sandra; Teixeira, Fábio G.; Mendes-Pinheiro, Bárbara; Domingues, Ana V.; Vilaça-Faria, Helena; Marote, Ana; Silva, DeolindaParkinson’s disease (PD) is the second most common age-related neurodegenerative disorder. The neurodegeneration leading to incapacitating motor abnormalities mainly occurs in the nigrostriatal pathway due to the loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc). Several animal models have been developed not only to better understand the mechanisms underlying neurodegeneration but also t...
Bone Marrow Mesenchymal Stem Cells' Secretome Exerts Neuroprotective Effects in...
Mendes-Pinheiro, Bárbara; Anjo, Sandra I.; Manadas, Bruno; Da Silva, Jorge D.; Marote, Ana; Behie, Leo A.; Teixeira, Fábio G.; Salgado, António J.Parkinson's disease (PD) is characterized by a selective loss of dopamine (DA) neurons in the human midbrain causing motor dysfunctions. The exact mechanism behind dopaminergic cell death is still not completely understood and, so far, no cure or neuroprotective treatment for PD is available. Recent studies have brought attention to the variety of bioactive molecules produced by mesenchymal stem cells (MSCs), g...
Filling the gap: Neural stem cells as a promising therapy for spinal cord injury
Pereira, Inês M.; Marote, Ana; Salgado, A. J.; Silva, Nuno A.Spinal cord injury (SCI) can lead to severe motor, sensory and social impairments having a huge impact on patients’ lives. The complex and time-dependent SCI pathophysiology has been hampering the development of novel and effective therapies. Current treatment options include surgical interventions, to stabilize and decompress the spinal cord, and rehabilitative care, without providing a cure for these patients...
Generation of an induced pluripotent stem cell line (CSC-46) from a patient wit...
Gustavsson, Nadja; Marote, Ana; Pomeshchik, Yuriy; Russ, Kaspar; Azevedo, Carla; Chumarina, Margarita; Goldwurm, Stefano; Collin, Anna; Pinto, LuísaMutations in the glucocerebrosidase (GBA) gene have been associated with the development of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line was generated from a 60-year old patient diagnosed with PD and carrying a new mutation variant p.R301C in GBA. Using non-integrating Sendai virus-based technology, we utilized OCT3/4, SOX2, c-MYC and KLF4 transcription factors to reprogram skin fibrob...
Generation of an induced pluripotent stem cell line (CSC-41) from a Parkinson's...
Marote, Ana; Pomeshchik, Yuriy; Collin, Anna; Goldwurm, Stefano; Lamas, Nuno J.; Pinto, Luísa; Salgado, A. J.; Roybon, LaurentThe leucine-rich repeat kinase 2 (LRRK2) p.G2019S mutation is the most common genetic cause of Parkinson's disease (PD). An induced pluripotent stem cell (iPSC) line CSC-41 was generated from a 75-year old patient diagnosed with PD caused by a p.G2019S mutation in LRRK2. Skin fibroblasts were reprogrammed using a non-integrating Sendai virus-based technology to deliver OCT3/4, SOX2, c-MYC and KLF4 transcription...
Children as caregivers: a research study on the perspective of nurses from the ...
Pinto, Carla; Marote, Ana; Pedrosa, Pedro; Barbieri-Figueiredo, MCEnquadramento: Não há registos, em Portugal, sobre crianças como cuidadoras. A literatura internacional realça que esta é uma área importante de intervenção dos profissionais de saúde. Objetivos: Analisar a perspetiva dos enfermeiros dos cuidados de saúde primários da Madeira sobre a problemática das crianças como cuidadoras e conhecer as áreas de intervenção de enfermagem junto destas. Metodologia: Estudo quan...
MSCs-Derived Exosomes: Cell-Secreted Nanovesicles with Regenerative Potential
Marote, Ana; Teixeira, Fábio Gabriel Rodrigues; Pinheiro, Bárbara Filipa Mendes; Salgado, A. J.Exosomes are membrane-enclosed nanovesicles (30-150 nm) that shuttle active cargoes between different cells. These tiny extracellular vesicles have been recently isolated from mesenchymal stem cells (MSCs) conditioned medium, a population of multipotent cells identified in several adult tissues. MSCs paracrine activity has been already shown to be the key mediator of their elicited regenerative effects. On the ...
A proteomic analysis of the interactions between poly(L-lactic acid) nanofibers...
Marote, Ana; Barroca, Nathalie; Vitorino, Rui; Silva, Raquel M.; Fernandes, Maria H.V.; Vilarinho, Paula M.; Silva, Odete A.B. da Cruz ePoly (L-lactic acid) (PLLA) is a biodegradable and biocompatible polymer that has been put forward as a promising material for therapeutic approaches aiming to restore neuronal function. The topographic cues present in PLLA-based scaffolds, defined by the technique used in their preparation, have been shown to play a role on the cellular behavior of adherent cells. Even though this interaction has been shown to...