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Nonthermal high-pressure microalgae extracts: A new source of natural ingredien...

Garcia, Mónica P.; Regueiras, Ana; Lopes, Graciliana; Matos, Gabriela; Silva, Lucília P. da; Cerqueira, Mariana T.; Cardoso, Helena; Correia, Nádia

Microalgae are one of the most prominent sources of ingredients for the cosmetic industry not only due to their diversity but, importantly, due to the low environmental impact in production and extraction. A sustainable extraction process requires the use of effective and environmentally friendly solvents that guarantee an extract with a relevant content and in high yields. In this work, Chlorella vulgaris, Nan...


Convection patterns gradients of non-living and living micro-entities in hydrogels

Canadas, Raphael Faustino; Patrício, Pedro; Brancato, Virginia; Gasperini, Luca; Caballero, David; Pires, Ricardo A; Costa, João B.; Pereira, Hélder

Inducing thermal gradients in two injected fluid systems results in the temporal formation of mixing conductive streams. If preserved through sol-gel transition, this mechanism can be used to drive and pattern non-living and living entities in mixed hydrogels. Interfaces are vital in nature, where gradients of non-living and living entities build distinct yet continuous integrated living tissues. However, the c...


Tailoring gellan gum spongy-like hydrogels’ microstructure by controlling freez...

Moreira, Helena R.; Silva, Lucília P. da; Reis, R. L.; Marques, Alexandra P.

Gellan gum (GG) spongy-like hydrogels have been explored for different tissue engineering (TE) applications owing to their highly attractive hydrogel-like features, and improved mechanical resilience and cell performance. Although the whole process for the preparation of these materials is well-defined, we hypothesized that variations occurring during the freezing step lead to batch-to-batch discrepancies. Aimi...


Silk-based anisotropical 3D biotextiles for bone regeneration

Ribeiro, Viviana P.; Silva-Correia, Joana; Nascimento, Ana I.; Morais, Alain da Silva; Marques, Alexandra P.; Ribeiro, Ana S.; Silva, Carla J.

Bone loss in the craniofacial complex can been treated using several conventional therapeutic strategies that face many obstacles and limitations. In this work, novel three-dimensional (3D) biotextile architectures were developed as a possible strategy for flat bone regeneration applications. As a fully automated processing route, this strategy as potential to be easily industrialized. Silk fibroin (SF) yarns w...


Modulating cell adhesion to polybutylene succinate biotextile constructs for ti...

Ribeiro, Viviana P.; Almeida, Lília R.; Martins, Ana R.; Pashkuleva, Iva; Marques, Alexandra P.; Ribeiro, Ana S.; Silva, Carla J.; Bonifácio, Graça

Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineering applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the s...


Influence of different surface modification treatments on silk biotextiles for ...

Ribeiro, Viviana P.; Almeida, Lília R.; Martins, Ana R.; Pashkuleva, Iva; Marques, Alexandra P.; Ribeiro, Ana S.; Silva, Carla J.; Bonifácio, Graça

Biotextile structures from silk fibroin have demonstrated to be particularly interesting for tissue engineering (TE) applications due to their high mechanical strength, interconnectivity, porosity, and ability to degrade under physiological conditions. In this work, we described several surface treatments of knitted silk fibroin (SF) scaffolds, namely sodium hydroxide (NaOH) solution, ultraviolet radiation expo...


Finely tuned fiber-based porous structures for bone tissue engineering applicat...

Ribeiro, Viviana; Silva-Correia, Joana; Morais, Alain; Correlo, Vitor M.; Marques, Alexandra P.; Ribeiro, Ana; Silva, Carla; Durães, Nelson


Textile-based silk scaffolds for bone tissue engineering applications

Ribeiro, Viviana; Morais, Alain; Correlo, Vitor M.; Marques, Alexandra P.; Ribeiro, Ana; Silva, Carla; Durães, Nuno; Bonifácio, Graça; Sousa, Rui

INTRODUCTION: Scaffolds developed for bone tissue engineering (TE) need to facilitate and promote cell adhesion, proliferation and neo-tissue formation. They must possess specific properties to allow the new tissue to integrate with the material, without inducing any inflammatory response. The intra-architectural scaffold geometry, porosity, scaffold material and surface area play important roles in this proces...


In vivo performance of chitosan/soy-based membranes as wound dressing devices f...

Santos, T. C.; Hoering, B.; Reise, Kathrin; Marques, Alexandra P.; Silva, Simone Santos; Oliveira, Joaquim M.; Mano, J. F.; Castro, António G.

Wound management represents a major clinical challenge on what concerns healing enhancement and pain control. The selection of an appropriate dressing plays an important role in both recovery and esthetic ap- pearance of the regenerated tissue. Despite the wide range of available dressings, the progress in the wound care market relies on the increasing interest in using natural-based biomedical products. Herein...


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