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Ram Semen Cryopreservation for Portuguese Native Breeds: Season and Breed Effec...

Barbas, João Pedro; Pimenta, Jorge; Baptista, Maria Conceição; Marques, Carla Cruz; Pereira, Rosa Maria Lino Neto; Simões, João

ABSTRACT - The semen quality is one of the determinant factors of ram semen cryopreservation. The present retrospective study aimed to characterize the seasonal ram pattern during the year for ten Portuguese local sheep breeds, hypothesizing that the breed and season had low effects on the main spermatozoa traits. A total of 1471 ejaculates were used and evaluated (fresh semen) from 85 rams between 2004 and 202...


Effect of a Novel Hydroxybenzoic Acid Based Mitochondria Directed Antioxidant M...

Santos, João Campos; Marques, Carla Cruz; Baptista, Maria Conceição; Pimenta, Jorge; Teixeira, José; Montezinho, Liliana; Cagide, Fernando

Sperm cells are particularly vulnerable to reactive oxygen species (ROS), impairing their fertilizing ability. Our objective was to study the effect of a novel mitochondrial-directed antioxidant, AntiOxBEN2, on bovine sperm function. This antioxidant was added to the semen capacitation medium (CAP), during the swim-up process, and to the fertilization medium (FERT) during the co-incubation of matured oocytes an...


Anti-Aging Effect of Urolithin A on Bovine Oocytes In Vitro

Fonseca, Élisa; Marques, Carla Cruz; Pimenta, Jorge; Jorge, Joana; Baptista, Maria Conceição; Gonçalves, Ana Cristina; Pereira, Rosa M. L. N.

Oxidative stress and mitochondrial dysfunction have been associated with the age-related decline of oocyte quality and strategies for their prevention are currently quested. Urolithin A (UA) is a natural metabolite with pro-apoptotic and antioxidant effects, capable of preventing the accumulation of dysfunctional mitochondria in different aged cells. UA has never been tested in bovine oocytes. Our aim was to st...


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