46 documents found, page 1 of 5
In-Situ Anaerobic Heating of Human Bones Probed by Neutron Diffraction
Festa, Giulia; Mamede, A. P.; Gonçalves, David; Cunha, Eugénia; Kockelmann, Winfried; Parker, Stewart F.; Batista de Carvalho, Luís A. E.The first neutron diffraction study of in-situ anaerobic burning of human bones is reported, aiming at an interpretation of heat-induced changes in bone, which were previously detected by vibrational spectroscopy, including inelastic neutron scattering techniques. Structural and crystallinity variations were monitored in samples of the human femur and tibia, as well as a reference hydroxyapatite, upon heating u...
Novel insights into mice multi-organ metabolism upon exposure to a potential an...
Carneiro, Tatiana J.; Araújo, Rita; Vojtek, Martin; Gonçalves-Monteiro, Salomé; Diniz, Carmen; Carvalho, Ana L. M. Batista de; Marques, M. Paula M.Pd(II)-compounds are presently regarded as promising anticancer drugs, as an alternative to Pt(II)-based drugs (e.g., cisplatin), which typically trigger severe side-effects and acquired resistance. Dinuclear Pd(II) complexes with biogenic polyamines such as spermine (Pd2Spm) have exhibited particularly beneficial cytotoxic properties, hence unveiling the importance of understanding their impact on organism met...
Metabolic aspects of palladium(II) potential anti-cancer drugs
Carneiro, Tatiana J.; Martins, Ana S.; Marques, M. Paula M.; Gil, Ana M.This mini-review reports on the existing knowledge of the metabolic effects of palladium [Pd(II)] complexes with potential anticancer activity, on cell lines and murine models. Most studies have addressed mononuclear Pd(II) complexes, although increasing interest has been noted in bidentate complexes, as polynuclear structures. In addition, the majority of records have reported in vitro studies on cancer cell l...
Potential of Bioapatite Hydroxyls for Research on Archeological Burned Bone
Mamede, Adriana P.; Vassalo, Ana R.; Piga, Giampaolo; Cunha, Eugénia; Parker, Stewart F.; Marques, M. Paula M.; Carvalho, Luís A. E. Batista deThe estimation of the maximum temperature affecting skeletal remains was previously attempted via infrared techniques. However, fossilization may cause changes in the composition of bones that replicate those from burned bones. We presently investigated the potential of three OH/P indices (intensity ratios of characteristic infrared bands for OH and phosphate groups, respectively) to identify bones burned at hi...
Vibrational and conformational studies of 1,3-diaminopropane and its N-deuterat...
Mendes, Sofia R. O.; Amado, Ana M.; Tomkinson, John; Marques, M. Paula M.; Carvalho, Luís A. E. Batista de
An EXAFS Approach to the Study of Polyoxometalate–Protein Interactions: The Cas...
Marques, M. Paula M.; Gianolio, Diego; Ramos, Susana; Carvalho, Luís A. E. Batista de; Aureliano, ManuelEXAFS and XANES experiments were used to assess decavanadate interplay with actin, in both the globular and polymerized forms, under different conditions of pH, temperature, ionic strength, and presence of ATP. This approach allowed us to simultaneously probe, for the first time, all vanadium species present in the system. It was established that decavanadate interacts with G-actin, triggering a protein conform...
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomi...
Lamego, Inês; Marques, M. Paula M.; Duarte, Iola F.; Martins, Ana S.; Oliveira, Helena; Gil, Ana M.A metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid m...
Raman spectroscopic evidence for the inclusion of decanoate ion in trimethyl-β-...
Seiça, Ana Filipa; Carvalho, Luís A. E. Batista de; Marques, M. Paula M.; Dias, José J. C. Teixeira
Characterization of decavanadate and decaniobate solutions by Raman spectroscopy
Aureliano, Manuel; Ohlin, C. André; Vieira, Michele O.; Marques, M. Paula M.; Casey, William H.; Carvalho, Luís A. E. Batista deThe decaniobate ion, (Nb10 = [Nb10O28](6-)) being isoelectronic and isostructural with the decavanadate ion (V10 = [V10O28](6-)), but chemically and electrochemically more inert, has been useful in advancing the understanding of V10 toxicology and pharmacological activities. In the present study, the solution chemistry of Nb10 and V10 between pH 4 and 12 is studied by Raman spectroscopy. The Raman spectra of V1...
Characterization of decavanadate and decaniobate solutions by Raman spectroscopy
Aureliano, M.; Ohlin, C. André; Vieira, Michele O.; Marques, M. Paula M.; Casey, William H.; Batista de Carvalho, Luís A. E.The decaniobate ion, (Nb10 = [Nb10O28]6−) being isoelectronic and isostructural with the decavanadate ion (V10 = [V10O28]6−), but chemically and electrochemically more inert, has been useful in advancing the understanding of V10 toxicology and pharmacological activities. In the present study, the solution chemistry of Nb10 and V10 between pH 4 and 12 is studied by Raman spectroscopy. The Raman spectra of V10 sh...