3 documents found, page 1 of 1
Structural and enzymatic characterization of Peruvianin‑I, the first germin-lik...
Cruz, Wallace T. da; Bezerra, Eduardo H. S.; Grangeiro, Thalles B.; Lopes, Jose L. S.; Silva, Maria Z. R.; Ramos, Márcio Viana; Rocha, Bruno A. M.The germin-like protein (GLP) purified from Thevetia peruviana, Peruvianin-I, is the only one described as having proteolytic activity. Therefore, the goal of this study was to investigate the structural features responsible for its enzymatic activity. Although the amino acid sequence of Peruvianin-I showed high identity with other GLPs, it exhibited punctual mutations, which were responsible for the absence of...
Biotechnological potential of a cysteine protease (CpCP3) from Calotropis proce...
Silva, Maria Z. R.; Oliveira, João P. B.; Ramos, Márcio Viana; Farias, Davi F.; Sá, Chayenne A. de; Ribeiro, Juliana A. C.; Silva, Ayrles F. B.This article reports the characterization and evaluation of the biotechnological potential of a cysteine protease purified from Calotropis procera (CpCP3). This enzyme was highly stable to different metal ions and was able to hydrolyze κ-casein similarly to bovine chymosin. Atomic force microscopy showed that the process of casein micelle aggregation induced by CpCP3 was similar to that caused by chymosin. The ...
Latex proteins from Calotropis procera: toxicity and immunological tolerance re...
Bezerra, Camila F.; Mota, Érika F.; Silva, Ana Claudia M.; Tomé, Adriana R.; Silva, Maria Z. R.; Brito, Daniel de; Porfírio, Camila T. M. N.Many thousands of plants are disseminated worldwide in traditional and folk medicines based on the belief that their leaves, roots, seeds, bark or secretions, when adequately handled, can treat, alleviate or ameliorate numerous disease symptoms. Calotropis procera (Apocynaceae) is a popular medicinal plant and the claims of this shrub's phytomedicinal properties have been scientifically validated. In this study...