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Development of Antifouling Strategies for Marine Applications

Filipe Mergulhão; Maria João Romeu

Marine biofouling is an undeniable challenge for aquatic systems since it is responsible for several environmental and ecological problems and economic losses. Several strategies have been developed to mitigate fouling-related issues in marine environments, including developing marine coatings using nanotechnology and biomimetic models, and incorporating natural compounds, peptides, bacteriophages, or specific ...


The effect of biomimetic surfaces on single- and dual-species biofilms of Esche...

Rita Santos; Francisca Cardoso; Ana Azevedo; Maria João Romeu; Cristina Amador; Luciana Gomes; Kathryn Whitehead; Jelmer Sjollema; Mette Burmølle


How do Graphene Composite Surfaces Affect the Development and Structure of Mari...

Maria João Romeu; Luciana Gomes; Sousa Cardoso, F; Morais, J; Vitor Vasconcelos; Whitehead, KA; Pereira, MFR; Soares, OSGP; Filipe Mergulhão

The progress of nanotechnology has prompted the development of novel marine antifouling coatings. In this study, the influence of a pristine graphene nanoplatelet (GNP)-modified surface in cyanobacterial biofilm formation was evaluated over a long-term assay using an in vitro platform which mimics the hydrodynamic conditions that prevail in real marine environments. Surface characterization by Optical Profilome...


The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyano...

Maria João Romeu; Lima, M; Luciana Gomes; de Jong, ED; Morais, J; Vitor Vasconcelos; Pereira, MFR; Soares, OSGP; Sjollema, J; Filipe Mergulhão

The development of environmentally friendly antifouling strategies for marine applications is of paramount importance, and the fabrication of innovative nanocomposite coatings is a promising approach. Moreover, since Optical Coherence Tomography (OCT) is a powerful imaging technique in biofilm science, the improvement of its analytical power is required to better evaluate the biofilm structure under different s...


Hydrodynamic conditions affect the proteomic profile of marine biofilms formed ...

Maria João Romeu; Dominguez-Perez, D; Almeida, D; Morais, J; Araujo, MJ; Osorio, H; Alexandre Campos; Vitor Vasconcelos; Filipe Mergulhão

Proteomic studies on cyanobacterial biofilms can be an effective approach to unravel metabolic pathways involved in biofilm formation and, consequently, obtain more efficient biofouling control strategies. Biofilm development by the filamentous cyanobacterium Toxifilum sp. LEGE 06021 was evaluated on different surfaces, glass and perspex, and at two significant shear rates for marine environments (4 s(-1) and 4...


Bacterial Adhesion and Biofilm Formation: Hydrodynamics Effects

Luciana Gomes; Rita Teixeira dos Santos; Maria João Romeu; Filipe Mergulhão

<jats:title>Abstract</jats:title><jats:p>The effectiveness of biomedical surfaces may be highly affected by the hydrodynamic condition. Surfaces releasing antimicrobial substances when exposed to flow may exhibit shorter lifetimes than at static conditions. Likewise, depending on the fluid flow surrounding the surface, contact-killing surfaces that are adhesive for bacterial cells may be covered by bacterial de...


Quantitative proteomic analysis of marine biofilms formed by filamentous cyanob...

Maria João Romeu; D. Domínguez-Pérez; D. Almeida; J. Morais; M. J. Araújo; H. Osório; A. Campos; V. Vasconcelos; Filipe Mergulhão

Cyanobacterial molecular biology can identify pathways that affect the adhesion and settlement of biofouling organisms and, consequently, obtain novel antifouling strategies for marine applications. Proteomic analyses can provide an essential understanding of how cyanobacteria adapt to different environmental settings. However, only a few qualitative studies have been performed in some cyanobacterial strains. C...


Unveiling the Antifouling Performance of Different Marine Surfaces and Their Ef...

Faria, SI; Teixeira Santos, R; Maria João Romeu; Morais, J; De Jong, E; Sjollema, J; Vitor Vasconcelos; Filipe Mergulhão

Since biofilm formation by microfoulers significantly contributes to the fouling process, it is important to evaluate the performance of marine surfaces to prevent biofilm formation, as well as understand their interactions with microfoulers and how these affect biofilm development and structure. In this study, the long-term performance of five surface materials-glass, perspex, polystyrene, epoxy-coated glass, ...


Characterization of planktonic and biofilm cells from two filamentous cyanobact...

Maria João Romeu; Dany Domínguez-Pérez; Daniela Almeida; João Morais; Alexandre Campos; Vítor Vasconcelos; Filipe Mergulhão

Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s(-1)and 40 s(-1)). For both strains and surfaces, biofilm development was higher at 4 s(-1). Biofi...


Biofilm formation by a cyanobacterium strain belonging to a distinct Leptolyngb...

Filipe Mergulhão; Maria João Romeu; Patrícia Alves; João Morais; Vitor Ramos; Vitor Vasconcelos


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