16 documents found, page 1 of 2

Teaching open and reproducible scholarship: A critical review of the evidence b...

In recent years, the scientific community has called for improvements in the credibility, robustness and reproducibility of research, characterized by increased interest and promotion of open and transparent research practices. While progress has been positive, there is a lack of consideration about how this approach can be embedded into undergraduate and postgraduate research training. Specifically, a critical...

Assessing the Impact of Prophylactic Vaccines on HPV Prevalence

Made available in DSpace on 2021-07-14T10:36:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-09-16. Added 1 bitstream(s) on 2021-07-14T11:34:05Z : No. of bitstreams: 1 S2179-84512019000200305.pdf: 956947 bytes, checksum: 8ef2ee7224d9d74190b379e2c4bfc3c5 (MD5); Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); PAPIIT-UNAM; Um modelo matemático á proposto para avaliar as estratégias de cont...

Neoadjuvant use of Imiquimod Before Surgery in Extramammary Paget’s Disease

Extramammary Paget's disease (EMPD) is a distinct form of a rare malignant skin neoplasm, primarily seen in postmenopausal Caucasian women. Surgery is the most common treatment. However, concerning vulvar EMPD, radical surgery can be mutilating and leads to loss of femininity. Recent therapeutic development has included medical and medical-surgical approaches, allowing better preservation of genital anatomy wit...

Analysis of DNA damage and repair in Saccharomyces cerevisiae using the comet a...

In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. We have assessed DNA damage and repair using the comet assay, which displayed a dose-response relationship with different DNA-damaging agents. Subsequently, we used this system to assess the antigenotoxic properties of a leaf extract from Ginkgo biloba (GBE). Ex...

Analysis of DNA damage and repair in saccharomyces cerevisiae using the comet a...

In this work we used the model organism Saccharomyces cerevisiae to characterise the biological activity and the mechanism of action of phytochemicals. One of the goals is to use mutant strains affected in basic mechanisms of oxidative stress response and DNA repair in order to uncover the molecular targets of phytochemicals. We have assessed DNA damage and repair using the comet assay, evaluated as “comet tail...

MAPK signaling pathways mediate acetic acid-induced cell death in saccharomyces...

Role of C2-phytoceramide in mediating cell death in yeast

Ceramides are naturally occurring sphingolipids that cause several biological effects, including induction of cell cycle arrest, cell maturation, terminal cell differentiation, cell senescence, and cell death. It is well established that ceramide induces both apoptosis and autophagy in mammalian cells. However, the molecular mechanisms induced by ceramide in yeast, as well as the signalling systems involved, ar...

Stimulation of DNA repair in saccharomyces cerevisiae by ginkgo biloba leaf ext...

Many extracts prepared from plants traditionally used for medicinal applications contain a variety of phytochemicals with antioxidant and antigenotoxic activity. In this work we measured the DNA protective effect of extracts of Ginkgo biloba leaves from oxidative stress using Saccharomyces cerevisiae as experimental model. The extract improved viability of yeast cells under oxidative stress imposed by hydrogen ...

Measuring oxidative DNA damage and DNA repair using the yeast comet assay

Chromosomal DNA damage can be a result of several processes and agents of endogenous or exogenous origin. These cause strand breaks or oxidized bases that lead to strand breaks, which relax the normally supercoiled genomic DNA and increase its electrophoretic mobility. The extent of DNA damage can be assessed by single cell gel electrophoresis, where the chromosomal DNA migration distance correlates with the ex...

Vacuole-mitochondrial cross-talk during apoptosis in yeast: a model for underst...

The yeast apoptosis field emerged with the finding that key components of the apoptotic machinery are conserved in these simple eukaryotes. Thus it became possible to exploit these genetically tractable organisms to improve our understanding of the intricate mechanisms of cell death in higher eukaryotes and of severe human diseases associated with apoptosis dysfunctions. Early on, it was recognized that a mitoc...