5 documents found, page 1 of 1
DEFB1 gene polymorphisms and tuberculosis in a Northeastern Brazilian population
Silva,Ronaldo Celerino da; Cruz,Heidi Lacerda Alves da; Brandão,Lucas André Cavalcanti; Guimarães,Rafael Lima; Montenegro,Lilian Maria LapaAbstract β-Defensin-1, an antimicrobial peptide encoded by the DEFB1 gene, is known to play an important role in lung mucosal immunity. In our association study we analyzed three DEFB1 functional polymorphisms -52G>A (rs1799946), -44C>G (rs1800972) and -20G>A (rs11362) in 92 tuberculosis patients and 286 healthy controls, both from Northeast Brazil: no association was found between the studied DEFB1 polymorphis...
Bone tuberculosis: a case report on child
Santos,Fabiana Cristina Fulco; Nascimento,André Luiz Alves do; Lira,Laís Ariane de Siqueira; Lima,Juliana Figueirêdo da CostaThe authors report a case of a 12-year-old child with a complaint of pain and deformity in the lower thoracic region that had lasted for two years. Clinical, epidemiological and laboratory characteristics associated with images of apparent damage in the T9-T10 and T11-T12 vertebrae taken by radiography of the thoracic spine and nuclear magnetic resonance in addition to the positivity of the molecular test based...
Rapid detection and differentiation of mycobacterial species using a multiplex ...
Lima,Andrea Santos; Duarte,Rafael Silva; Montenegro,Lilian Maria Lapa; Schindler,Haiana CharifkerIntroduction The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods The purpose of this study was to evaluate a multiplex polymerase chain reaction system using mycobacterial strains as an auxiliary tool in the differential d...
The performance of an in-house nested-PCR technique for pleural tuberculosis di...
Montenegro,Lilian Maria Lapa; Silva,Bruno Cesar da; Lima,Juliana Figueiredo da Costa; Cruz,Heidi Lacerda Alves da; Montenegro,Rosana de AlbuquerqueIntroduction This study evaluated the performance of an in-house nested-PCR system for the detection of the Mycobacterium tuberculosis complex in pleural fluid, blood and urine samples from pleural effusion tuberculosis patients by health services physicians in Pernambuco, Brazil. Methods A prospective double-blind study with 37 hospitalized patients of both sexes, aged over 15, was used to investigate the diag...
Detection of Mycobacterium tuberculosis complex by nested polymerase chain reac...
Furini,Adriana Antônia da Cruz; Pedro,Heloisa da Silveira Paro; Rodrigues,Jean Francisco; Montenegro,Lilian Maria Lapa; Machado,Ricardo Luiz DantasOBJECTIVE: To compare the performance of nested polymerase chain reaction (NPCR) with that of cultures in the detection of the Mycobacterium tuberculosis complex in pulmonary and extrapulmonary specimens.METHODS: We analyzed 20 and 78 pulmonary and extrapulmonary specimens, respectively, of 67 hospitalized patients suspected of having tuberculosis. An automated microbial system was used for the identification o...