7 documents found, page 1 of 1
Effect of thermal and chemical treatments used for SARS-COV-2 inactivation in t...
Lamy, Elsa; Rubio, Camila; Carreira, Laura; Capela e Silva, Fernando; Martinez-Subiela, Silvia; Tecles, Fernando; Lopez-Jornet, Pia; Céron, JoseThe present study aims to assess the effects of thermal and chemical inactivating procedures, that can be used for SARS-CoV-2 inactivation, on different salivary analytes. SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profile and a panel of 25 specific biomarkers of oxidative status, stress, metabolism and tissue damage were evaluated in samples subjected to different treatments: thermal (65 °C or 9...
Changes in the Saliva Proteome of Pigs with Diarrhoea Caused by Escherichia coli
Rodrigues, Miguel; López-Martinez, Maria Jose; Ortin-Bustillo, Alba; Cerón, Jose Joaquim; Martinez-Subiela, Silvia; Muñoz-Prieto, Alberto; Lamy, ElsaEscherichia coli represents the main cause of diarrhoea in pigs. Saliva can provide information about the pathophysiology of diseases and be a source of biomarkers. We aimed to identify changes in the salivary proteome of pigs with diarrhoea caused by E. coli. Saliva samples were collected from 10 pigs with this disease and 10 matched healthy controls. SDS-PAGE (1DE) and two-dimensional gel electrophoresis (2DE...
Saliva in Health and Disease - The Present and Future of a Unique Sample for Di...
Tvarijonaviciute, Asta; Martinez-Subiela, Silvia; Lopez-Jornet, Pia; Lamy, ElsaSaliva as a unique sample for health assessment is gaining attention among researchers of different fields in the last 20 years; being reflected in an impressive increase in the number of papers published studying saliva from different biological aspects in human and veterinary species. Once deemed merely a digestive juice is now considered a biological fluid capable of communicating information about physiopat...
Analytical validation of an automated assay for the measurement of adenosine de...
Franco-Martinéz, Lorena; Tecles, Fernando; Torres-Cantero, A.; Bernal, E.; San-Lázaro, I.; Alcaraz, M.J.; Vicente-Romero, M.R.; Lamy, ElsaObjectives The aim of the present study was to validate a commercially available automated assay for the measurement of total adenosine deaminase (tADA) and its isoenzymes (ADA1 and ADA2) in saliva in a fast and accurate way, and evaluate the possible changes of these analytes in individuals with SARS-CoV-2 infection. Methods The validation, in addition to the evaluation of precision and accuracy, included the ...
Salivary biomarkers in the diagnosis and monitoring of metabolic and endocrine ...
Tvarijonaviciute, Asta; Martinez-Subiela, Silvia; Lopez-Jornet, Pia; Lamy, ElsaThe present chapter revises the current knowledge about the use of salivary biomarkers for metabolic and endocrine disease diagnosis and monitoring in humans and different animal species. Currently, most information available comes from human medicine and studies performed with experimental animals such as mouse or rat, and less from other veterinary species. Furthermore, since obesity and its-related pathologi...
Changes in biochemical analytes in female dogs with subclinical Ancylostoma spp...
Schmidt, Elizabeth M.S. [UNESP]; Tvarijonaviciute, Asta; Martinez-Subiela, Silvia; Cer�n, Jos� J.; Eckersall, Peter D.Made available in DSpace on 2018-12-11T17:07:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-13; Background: Ancylostoma spp. is one of the most prevalent canine intestinal nematode infections which usually causes subclinical disease in adult dogs and has zoonotic implications. Therefore, the aim of this study was to explore and evaluate the possible pathophysiological changes that Ancylostoma spp....
Development and validation of an assay for measurement of leptin in pig saliva
Schmidt, Elizabeth M.S. [UNESP]; Escribano, Damián; Martinez-Subiela, Silvia; Martinez-Miró, Silvia; Hernández, Fuensanta; Tvarijonaviciute, AstaMade available in DSpace on 2018-12-11T17:07:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-28; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Background: Leptin has been measured in human in saliva samples. However, the low leptin concentration found in this biological fluid makes necessary the use of high sensitive methods. To the authors' knowledge, leptin has not been me...