9 documents found, page 1 of 1
Impact of Conventional and Potential New Metal-Based Drugs on Lipid Metabolism ...
Bispo, Daniela S. C.; Correia, Marlene; Carneiro, Tatiana J.; Martins, Ana S.; Reis, Aliana A. N.; Carvalho, Ana L. M. Batista de; Marques, Maria P. M.This work investigated the mechanisms of action of conventional drugs, cisplatin and oxaliplatin, and the potentially less deleterious drug Pd2Spermine (Spm) and its Pt(II) analog, against osteosarcoma MG-63 cells, using nuclear-magnetic-resonance metabolomics of the cellular lipidome. The Pt(II) chelates induced different responses, namely regarding polyunsaturated-fatty-acids (increased upon cisplatin), sugge...
Response of Osteosarcoma Cell Metabolism to Platinum and Palladium Chelates as ...
Martins, Ana S.; Batista de Carvalho, Ana L. M.; Marques, Maria Paula M.; Gil, Ana MThis paper reports the first metabolomics study of the impact of new chelates Pt2Spm and Pd2Spm (Spm = Spermine) on human osteosarcoma cellular metabolism, compared to the conventional platinum drugs cisplatin and oxaliplatin, in order to investigate the effects of different metal centers and ligands. Nuclear Magnetic Resonance metabolomics was used to identify meaningful metabolite variations in polar cell ext...
Metabolic Aspects of Palladium(II) Potential Anti-Cancer Drugs
Carneiro, Tatiana J.; Martins, Ana S.; Marques, Maria P. M.; Gil, Ana M.This mini-review reports on the existing knowledge of the metabolic effects of palladium [Pd(II)] complexes with potential anticancer activity, on cell lines and murine models. Most studies have addressed mononuclear Pd(II) complexes, although increasing interest has been noted in bidentate complexes, as polynuclear structures. In addition, the majority of records have reported in vitro studies on cancer cell l...
Metabolic aspects of palladium(II) potential anti-cancer drugs
Carneiro, Tatiana J.; Martins, Ana S.; Marques, M. Paula M.; Gil, Ana M.This mini-review reports on the existing knowledge of the metabolic effects of palladium [Pd(II)] complexes with potential anticancer activity, on cell lines and murine models. Most studies have addressed mononuclear Pd(II) complexes, although increasing interest has been noted in bidentate complexes, as polynuclear structures. In addition, the majority of records have reported in vitro studies on cancer cell l...
West Nile virus capsid protein interacts with biologically relevant host lipid ...
Martins, Ana S.; Carvalho, Filomena Almeida; Faustino, André F.; Martins, Ivo C.; Santos, Nuno C.West Nile and dengue viruses are closely related flaviviruses, originating mosquito-borne viral infections for which there are no effective and specific treatments. Their capsid proteins sequence and structure are particularly similar, forming highly superimposable α-helical homodimers. Measuring protein-ligand interactions at the single-molecule level yields detailed information of biological and biomedical re...
Methods for lipid droplet biophysical characterization in Flaviviridae infections
Martins, Ana S.; Martins, Ivo C.; Santos, Nuno C.Lipid droplets (LDs) are intracellular organelles for neutral lipid storage, originated from the endoplasmic reticulum. They play an essential role in lipid metabolism and cellular homeostasis. In fact, LDs are complex organelles, involved in many more cellular processes than those initially proposed. They have been extensively studied in the context of LD-associated pathologies. In particular, LDs have emerged...
Impact of the Pd2Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomi...
Lamego, Inês; Marques, M. Paula M.; Duarte, Iola F.; Martins, Ana S.; Oliveira, Helena; Gil, Ana M.A metabolomics study of Pd2Spermine(Spm) on osteosarcoma MG-63 and osteoblastic HOb cells is presented to assess the impact of the potential palladium drug on cell metabolism compared with cisplatin (cDDP). Despite its higher cytotoxicity, Pd2Spm induced lower (and reversible) metabolic impact on MG-63 cells and the absence of apoptosis; conversely, it induced significant deviations in osteoblastic amino acid m...
Prediction of Gestational Diabetes through NMR Metabolomics of Maternal Blood
Pinto, Joana; Almeida, Lara M.; Martins, Ana S.; Duarte, Daniela; Barros, Antonio S.; Gahano, Eulalia; Pita, Cristina; Almeida, Maria do CeuMetabolic biomarkers of pre- and postdiagnosis gestational diabetes mellitus (GDM) were sought, using nuclear magnetic resonance (NMR) metabolomics of maternal plasma and corresponding lipid extracts. Metabolite differences between controls and disease were identified through multivariate analysis of variable selected H-1 NMR spectra. For postdiagnosis GDM, partial least squares regression identified metabolite...
Contaminação, por ovos de helmintes encontrados em fezes de canídeos, de alguns...
Martins, Ana S.; Crespo, Maria Virgínia; Pereira, Ana Silva; Jorge, Ana TeresaFoi efectuado um estudo com o objectivo de determinar a provável contaminação parasitária, por ovos de helmintes, de parques e jardins públicos da cidade de Santarém. Procedeu-se à colheita aleatória de 144 amostras de fezes de canídeos, encontradas no solo de diferentes locais das quatro freguesias da cidade (Marvila, Sta. Iria, S. Salvador e S. Nicolau). As amostras foram sujeitas a análises coprológicas qual...