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Does a ghrelin stimulus during zebrafish embryonic stage modulate its performan...

Navarro-Guillén, Carmen; Dias, Jorge; Rocha, Filipa; Castanheira, M. F.; Martins, Catarina I. M.; Laizé, Vincent; Gavaia, Paulo; Engrola, Sofia

Metabolic programming refers to the induction, deletion, or impaired development of a somatic structure or "setting" of a physiological system by an early life stimulus operated at a critical period during development. Ghrelin is the only known orexigenic gut hormone, is an acylated peptide that acts as an endogenous ligand specific for growth-hormone secretagogue-receptor. The aim of the present work was to ev...


Cortisol and testosterone accumulation in a low pH recirculating aquaculture sy...

Mota, Vasco; Martins, Catarina I. M.; Eding, Ep H.; Canario, Adelino V. M.; Verreth, Johan A. J.

Steroids accumulate in recirculating aquaculture system (RAS), although explanatory factors for such accumulation are still poorly explored. This study investigated the effect of water exchange rate and pH in six replicated RAS on the concentration of the stress hormone cortisol in rainbow trout blood plasma and in the holding water and of the sex steroids testosterone, 11-ketotestosterone (11-KT) and 17,20-dih...


Water cortisol and testosterone in Nile tilapia (Oreochromis niloticus) recircu...

Mota, Vasco; Martins, Catarina I. M.; Eding, Ep H.; Canario, Adelino; Verreth, Johan A. J.

The accumulation of steroids released by fish in recirculating aquaculture systems (RAS) may potentially influence their physiology and behavior. The present study examined the release rate of cortisol and testosterone by Nile tilapia, Oreochromis niloticus, and their accumulation in six identical lab scale RAS operated at different water exchange rates (150 L/kg feed/day, (LowRAS) and 1500 L/kg feed/day, (High...


Are coping styles consistent in the teleost fish Sparus aurata through sexual m...

Castanheira, Maria Filipa; Martinez Paramo, S.; Figueiredo, F.; Cerqueira, Marco; Millot, Sandie; Oliveira, Catarina C. V.; Martins, Catarina I. M.

Individual differences in behaviour and physiological responses to stress are associated with evolutionary adaptive variation and thus raw material for evolution. In farmed animals, the interest in consistent trait associations, i.e. coping styles, has increased dramatically over the last years. However, one of limitations of the available knowledge, regarding the temporal consistency, is that it refers always ...


Are personality traits consistent in fish? The influence of social context

Castanheira, Maria Filipa; Cerqueira, Marco; Millot, Sandie; Gonçalves, Rui A.; Oliveira, Catarina C. V.; Conceição, Luis E. C.; Martins, Catarina I. M.

Individual differences in behavioural and physiological responses to challenges are progressively accepted as adaptive variation and reveal a strong degree of evolutionary conservation throughout the vertebrate taxa. Previous studies in gilthead seabream (Sparus aurata) suggested that individual differences in behaviour reflect distinct coping styles or personality, contrasting consistent traits associations. O...


Behavioural Stress Responses Predict Environmental Perception in European Sea B...

Millot, Sandie; Cerqueira, Marco; Castanheira, Maria-Filipa; Øverli, Øyvind; Oliveira, Rui F.; Martins, Catarina I. M.

Individual variation in the response to environmental challenges depends partly on innate reaction norms, partly on experience-based cognitive/emotional evaluations that individuals make of the situation. The goal of this study was to investigate whether pre-existing differences in behaviour predict the outcome of such assessment of environmental cues, using a conditioned place preference/avoidance (CPP/CPA) pa...


Use of conditioned place preference/avoidance tests to assess affective states ...

Millot, Sandie; Cerqueira, Marco; Castanheira, Maria Filipa; Overli, Oyvind; Martins, Catarina I. M.; Oliveira, Rui Filipe

Animal welfare has been defined as the balance between positive and negative experiences or affective states. Despite the growing evidence of complex cognitive abilities and the expression of affective states such as pain and fear, very little is known about ability to experience memory based affective states in non-mammalian animal models. The goal of this study was to validate conditioned place preference/avo...


Steroids accumulate in the rearing water of commercial recirculating aquacultur...

Mota, Vasco; Martins, Catarina I. M.; Eding, Ep; Canario, Adelino V. M.; Verreth, Johan

Little information is available on steroid concentrations in the rearing water of aquaculture systemsand whether they accumulate in recirculating aquaculture systems (RAS). Therefore this study aimedat determining (1) the concentrations and variation of cortisol and sex steroids in RAS, (2) the con-tribution of fish rearing conditions to steroid concentrations in seven commercial RAS. Each RAS wassampled twice ...


Behavioural stress responses predict environmental perception in European sea b...

Millot, Sandie; Cerqueira, Marco; Castanheira, Maria Filipa; Overli, Oyvind; Oliveira, Rui F.; Martins, Catarina I. M.

Individual variation in the response to environmental challenges depends partly on innate reaction norms, partly on experience-based cognitive/emotional evaluations that individuals make of the situation. The goal of this study was to investigate whether pre-existing differences in behaviour predict the outcome of such assessment of environmental cues, using a conditioned place preference/avoidance (CPP/CPA) pa...


Can we predict personality in fish? searching for consistency over time and acr...

Castanheira, Maria Filipa; Herrera, Marcelino; Costas, Benjamin; Conceicao, Luis E. C.; Martins, Catarina I. M.

The interest in animal personality, broadly defined as consistency of individual behavioural traits over time and across contexts, has increased dramatically over the last years. Individual differences in behaviour are no longer recognised as noise around a mean but rather as adaptive variation and thus, essentially, raw material for evolution. Animal personality has been considered evolutionary conserved and h...


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