12 documents found, page 1 of 2
Experimental benznidazole treatment of Trypanosoma cruzi II strains isolated fr...
Silva, Jaquelline Carla Valamiel de Oliveira e; Assis, Girley Francisco Machado de; Oliveira, Maykon Tavares de; Paiva, Nívia Carolina Nogueira deTrypanosoma cruzi strains from distinct geographic areas show differences in drug resistance and association between parasites genetic and treatment response has been observed. Considering that benznidazole (BZ) can reduce the parasite burden and tissues damage, even in not cured animals and individuals, the goal is to assess the drug response to BZ of T. cruzi II strains isolated from children of the Jequitinh...
Trypanosoma cruzi Discret Typing Units (TcII and TcVI) in samples of patients f...
Oliveira, Maykon Tavares de; Assis, Girley Francisco Machado de; Silva, Jaquelline Carla Valamiel de Oliveira e; Machado, Evandro Marques de MenezesBackground: Trypanosoma cruzi is classified into six discrete taxonomic units (DTUs). For this classification, different biological markers and classification criteria have been used. The objective was to identify the genetic profile of T. cruzi samples isolated from patients of two municipalities of Jequitinhonha Valley, MG, Brazil. Methods: Molecular characterization was performed using two different criteria...
Development of a label-free immunosensor based on surface plasmon resonance tec...
Souto, Dênio Emanuel Pires; Silva, Jussara Vieira; Martins, Helen Rodrigues; Reis, Alexandre Barbosa; Luz, Rita de Cássia Silva; Kubota, Lauro TatsuoIn this work ,a surface Plasmon resonance (SPR) immune sensor was developed using an 11-mercaptoundecanoic acid (11-MUA) modified gold SPR sensor chip for the detection of anti- Leishmania infantum antibodies. The soluble antigens of L. infantum were securely immobilized on an SPR gold disk byan 11-MUA self-assembled monolayer. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning e...
Molecular and biological characterization of Trypanosoma cruzi strains isolated...
Silva, Jaquelline Carla Valamiel de Oliveira e; Assis, Girley Francisco Machado de; Oliveira, Maykon Tavares de; Valadares, Helder Magno SilvaThe biological diversity of Trypanosoma cruzi strains plays an important role in the clinical and epidemiological features of Chagas disease. Methods: Eight T. cruzi strains isolated from children living in a Chagas disease vector-controlled area of Jequitinhonha Valley, State of Minas Gerais, Brazil, were genetically and biologically characterized. Results: The characterizations demonstrated that all of the st...
Clinical and laboratory status of patients with chronic Chagas disease living i...
Lana, Marta de; Lopes, Leonardo de Araújo; Martins, Helen Rodrigues; Bahia, Maria Terezinha; Assis, Girley Francisco Machado deTwenty-eight Chagas disease patients (CD), 22 with the indeterminate clinical form (IND) and six with the cardiac or digestive form (CARD/DIG), were treated with benznidazole and underwent clinical and laboratorial analysis before (IND and CARD/DIG) and nine years after [patients after treatment (CDt), patients with the indeterminate clinical form at treatment onset (INDt) and with the cardiac or digestive form...
In-house ELISA method to analyze anti-Trypanosoma cruzi IgG reactivity for diff...
Santos, Lilian da Silva; Torres, Rosália Morais; Assis, Girley Francisco Machado de; Bahia, Maria Terezinha; Martins, Helen RodriguesO objetivo foi desenvolver um método sorológico in-house de alta especificidade e sensibilidade para diagnosticar e monitorar a morbidade da doença de Chagas. Métodos: Para tal, a reatividade sorológica de IgG e subclasses foi testada em soros de pacientes chagásicos de Berilo, Vale do Jequitinhonha/MG/Brasil. A reatividade sorológica foi também avaliada em amostras de pacientes com outras doenças infecto-conta...
Tratamento com benzonidazol em camundongos infectados com misturas de populaçõe...
Martins, Helen RodriguesO objetivo desse trabalho foi avaliar o impacto de infecções mistas por clones de Trypanosoma cruzi pertencentes aos quatro genótipos principais na eficácia terapêutica do Benzonidazol (BZ) em camundongos BALB/c. Para esta proposta, foram empregados dois clones do T. cruzi de cada genótipo com diferentes níveis de virulência e de susceptibilidade ao BZ, que foram combinados aos pares totalizando 24 misturas dis...
Trypanosoma cruzi : treatment with the iron chelator desferrioxamine reduces pa...
Arantes, Jerusa Marilda; Pedrosa, Maria Lúcia; Martins, Helen Rodrigues; Veloso, Vanja Maria; Lana, Marta de; Bahia, Maria TerezinhaThe effects of prolonged treatment with iron chelator (desferrioxamine) on the development of infection in mice inoculated with Y Trypanosoma cruzi were determined. Infected/treated mice presented lower levels of parasitemia and reduced mortality rate compared with infected/non-treated animals. The five out of twenty infected/treated mice that survived the acute phase of infection showed negative hemoculture an...
Genetic modulation in Be-78 and Y Trypanosoma cruzi strains after long-term inf...
Veloso, Vanja Maria; Guedes, Paulo Marcos da Matta; Lana, Marta de; Martins, Helen Rodrigues; Carneiro, Cláudia MartinsThe genetic profile of Trypanosoma cruzi was evaluated in parasite populations isolated from Beagle dogs experimentally infected with Be-78 and Y strains that present distinct biological and genetic characteristics. Molecular characterization of the isolates obtained 30 days and 2 years after infection was carried out. For typing MLEE, sequence polymorphisms of the mitochondrial cytochrome oxidase subunit II ge...
Trypanosoma cruzi : immunoglobulin isotype profiles during the acute phase of c...
Vital, Wendel Coura; Carneiro, Cláudia Martins; Martins, Helen Rodrigues; Lana, Marta de; Veloso, Vanja Maria; Carvalho, Andréa Teixeira deA detailed investigation has been carried out about the serological profiles of groups of dogs experimen-tally infected with metacyclic (MT) or blood (BT) trypomastigotes of Berenice-78 Trypanosoma cruzi strain. Peripheral blood was collected from infected dogs and uninfected controls, weekly during 35 days following the acute phase of infection, and immunoglobulin profiles were determined by ELISA. Dogs infect...