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In vitro digestion and cytotoxicity study of cannabidiol-loaded nanostructured ...

Vardanega, Renata; Lüdtke, Fernanda Luisa; Loureiro, Luís; Gonçalves, Raquel F. S.; Martins, Joana T.; Pinheiro, A. C.; Vicente, A. A.

Cannabidiol (CBD), a lipophilic compound with promising health benefits, such as analgesic, anti-inflammatory and neuroprotective effects, is characterized by low oral bioavailability, which limit its therapeutic application. To address these limitations, CBD was incorporated into nanostructured lipid carriers (NLCs), either as CBD-rich extract (CBDext) or as an isolate (CBDiso). The resulting systems NLC-CBDex...


The effect of conventional and ohmic heating as pasteurization methods on the m...

Aleksandrovas, Elvidas; Vasiliauskaitè, Agnè; Vieira, Jorge Miguel Magalhães; Martins, Joana T.; Pereira, Ricardo Nuno Correia; Vicente, A. A.

Edible film-forming solutions typically undergo thermal treatment to ensure microbial safety before being applied to food products. The aim of this study was to assess the effects of two different heating methodsconventional heating (CH) and ohmic heating (OH)on the physical, chemical, and microbiological properties of liquid acid whey permeate (AWP) and liquid acid whey protein concentrate (AWPC) edible films....


Development of 3D-printed foods incorporating riboflavin-loaded whey protein is...

Araújo, João Miguel Fernandes; Fernandes, Jean Michel; Madalena, Daniel Alexandre Silva; Gonçalves, Raquel Filipa Silva; Vieira, Jorge Miguel Magalhães

3D printing has emerged as a groundbreaking technology, aiming to enhance sensory attributes and improving nutritional/functional aspects. Simultaneously, nano-delivery systems have emerged as an opportunity to protect bioactive compounds against degradation and improve their bioaccessibility. Therefore, a novel concept is underway, involving the 3D printing of perishable healthy foods previously fortified with...


Development of novel functional thickened drinks enriched with vitamin D3 for t...

Fernandes, Jean Michel; Vieira, Jorge Miguel Magalhães; Gonçalves, Raquel Filipa Silva; Martins, Joana T.; Vicente, A. A.; Pinheiro, A. C.

The older adult population is greatly affected by malnutrition. This condition can arise from inappropriate micro and micronutrient intake, as well as due to experienced age-related changes. For instance, a significant number of older adults suffer from dysphagia. Thus, the development of foods with adequate consistency/viscosity are vital for overcome this condition. Within this framework, novel functional thi...


Characterization of dairy industry secondary material-based edible films: effec...

Vasiliauskaite, Agne; Aleksandrovas, Elvidas; Martins, Joana T.; Vieira, Jorge Miguel Magalhães; Vicente, A. A.; Radenkovs, Vitalijs; Rud, Ida

Edible films are a promising method for delivering probiotics in food. The survival of Lacticaseibacillus paracasei A11 and Lactobacillus helveticus MI-LH13 (7 log CFU/g), which were cultivated in liquid whey permeate (LWP), in films made from liquid acid whey protein concentrate (M) or liquid acid whey permeate (Z), was evaluated over 21 days at 25 °C and 4 °C. Film drying and matrix type had varying effects o...


Production of chitosan-fish oil-green tea extract-based coating for Atlantic bo...

Lüdtke, Fernanda Luisa; Martins, Joana T.; Marx, Ítala Maria Gouveia; Vicente, A. A.; Vieira, Jorge Miguel Magalhães

Atlantic bonito (Sarda sarda) is a fish from Atlantic Ocean well-known for its pleasant taste and high nutritional value. However, it is susceptible to lipid and protein oxidation and spoilage. Sustainable bio-based edible coatings are increasingly a viable option for freshness preservation and to extend the shelf life of highly perishable food products. In this context, a coating formulation composed by chitos...


High lignin content polymer filaments as carbon fibre precursors

Ribeiro, Rui; Guerreiro, Miguel; Reis, Renato; Martins, Joana T.; Vieira, J.M.P.; Silva, Mariana Martins da; Covas, J. A.; Paiva, Maria C.

The growing environmental awareness, the search for alternatives to fossil resources, and the goal of achieving a circular economy have all contributed to the increasing valorization of biowaste to produce bio-based polymers and other high-value products. Among the various biowaste materials, lignin has gained significant attention due to its high aromatic carbon content, low cost, and abundance. Lignin is pred...


Enhancing cannabidiol bioaccessibility using ionic liquid as emulsifier to prod...

Vardanega, Renata; Lüdtke, Fernanda Luisa; Loureiro, Luís Carlos Sá; Hijo, Ariel A. C. Toledo; Martins, Joana T.; Pinheiro, A. C.; Vicente, A. A.

The emulsifying potential of a biocompatible ionic liquid (IL) to produce lipid-based nanosystems developed to enhance the bioaccessibility of cannabidiol (CBD) was investigated. The IL (cholinium oleate) was evaluated at concentrations of 1 % and 2 % to produce nanoemulsions (NE-IL) and nanostructured lipid carriers (NLC-IL) loaded with CBD. The IL concentration of 1 % demonstrated to be sufficient to produce ...


Physicochemical properties of a bioactive polysaccharide film from cassia grand...

Ferreira, Kétura; Cardoso, Kethylen; Costa, Romero Brandão; Martins, Joana T.; Botelho, C. M.; Neves, Anna; Nascimento, Thiago; Batista, Juanize

(1) Background: Polysaccharide films are promising vehicles for the delivery of bioactive agents such as collagenases, as they provide controlled release at the wound site, facilitating tissue regeneration. This study aimed to investigate the physicochemical properties of Cassia grandis polysaccharide films with immobilized collagenase from Streptomyces parvulus (DPUA/1573). (2) Methods: Galactomannan was extra...


Isolation and identification of lichen photobionts collected from different env...

Loureiro, Luís Carlos Sá; Morais, João; Silva, Raquel; Martins, Joana T.; Geada, Pedro Miguel Macedo; Vasconcelos, Vítor; Vicente, A. A.

Lichens are organisms constituted by a symbiotic relationship between a fungus (mycobiont) and a photoautotrophic partner (photobiont). Lichens produce several bioactive compounds; however, the biotechnological exploitation of this organism is hampered by its slow growth. To start studying the possibility of exploiting lichens as alternative sources of bioactive compounds, eighteen lichens were collected in the...


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