29 documents found, page 1 of 3
Cholesterol Functionalized Nanoparticles Are Effective against Helicobacter pyl...
Pinho, AS; Pereira, R; Pereira, M; Rai, A; Ferreira, L; Martins, MCL; Parreira, PHelicobacter pylori chronic infection is the highest risk factor for the development of gastric cancer, being this Gram-negative bacterium classified as carcinogenic. The mounting resistance of H. pylori to antibiotics calls for innovative therapeutic strategies. Here, the proof-of-concept studies that support the development of a "trojan horse" therapeutic strategy based on cholesterol-grafted nanoparticles (C...
One-Pot Microfluidics to Engineer Chitosan Nanoparticles Conjugated with Antimi...
Fonseca, D R; Alves, PM; Neto, E; Custodio, B; Guimaraes, S; Moura, D; Annis, F; Martins, M; Gomes, A; Teixeira, C; Gomes, P; Pereira, RF; Freitas, PSurface bioconjugation of antimicrobial peptides (AMP) onto nanoparticles (AMP-NP) is a complex, multistep, and time-consuming task. Herein, a microfluidic system for the one-pot production of AMP-NP was developed. Norbornene-modified chitosan was used for NP production (NorChit-NP), and thiolated-AMP was grafted on their surface via thiol-norbornene "photoclick" chemistry over exposure of two parallel UV LEDs....
Dhvar5-chitosan nanogels and their potential to improve antibiotics activity
Costa, B; Alves, PM; Fonseca, DR; Campos, F; Monteiro, AC; Shahrour, H; Gomes, A; Costa, F; Gomes, P; Martínez-de-Tejada, G; Monteiro, C; Martins, MCLInfection is one of the main causes of orthopedic implants failure, with antibiotic-resistant bacteria playing a crucial role in this outcome. In this work, antimicrobial nanogels were developed to be applied in situ as implant coating to prevent orthopedic-device-related infections. To that regard, a broad-spectrum antimicrobial peptide, Dhvar5, was grafted onto chitosan via thiol-norbornene "photoclick" chemi...
Paving the way for a non‐antibiotic and microbiota friendly therapy for Helicob...
Seabra, CL; Pinho, AS; Nunes, C; Amorim, I; Pedro, N; Henriques, P; Monteiro, C; Gomes, J; Machado, C; Gartner, F; Pereira, L; Reis, S; Reis, CABackground: The World Health Organization has identified Helicobacter pylori, a Gram-negative bacterium responsible for several gastric disorders, as one of the pathogenic bacteria that requires newer non-antibiotic approaches for its management. We previously demonstrated that nanostructured lipid carriers (NLC) loaded with docosahexaenoic acid (DHA-NLC)have excellent in vitro performance against H. pylori. Ma...
How to manage Helicobacter pylori infection beyond antibiotics: The bioengineer...
Fonseca, DR; Chitas, R; Parreira, P; Martins, MCLThe rise in antibiotic resistance rate challenges the effective treatment of various bacterial infections. One of them is the gastric infection caused by Helicobacter pylori, a Gram-negative bacterium responsible for several gastric disorders, as peptic ulcer, and close to 90 % of all gastric cancer cases. Available treatments, combining at least 3 antibiotics, fail in up to 40 % of the patients, prompting the ...
Peptide-based self-assembled monolayers (SAMs): what peptides can do for SAMs a...
Redondo-Gómez, C; Parreira, P; Martins, MCL; Azevedo, HSSelf-assembled monolayers (SAMs) represent highly ordered molecular materials with versatile biochemical features and multidisciplinary applications. Research on SAMs has made much progress since the early begginings of Au substrates and alkanethiols, and numerous examples of peptide-displaying SAMs can be found in the literature. Peptides, presenting increasing structural complexity, stimuli-responsiveness, an...
Dhvar5- and MSI78-coated titanium are bactericidal against methicillin-resistan...
Costa, B; Coelho, J; Silva, V; Shahrour, H; Costa, NA; Ribeiro, AR; Santos, SG; Costa, F; Martínez-de-Tejada, G; Monteiro, C; Martins, MCLInfection is one of the major issues associated with the failure of orthopedic devices, mainly due to implant bacterial colonization, biofilm formation, and associated antibiotic resistance. Antimicrobial peptides (AMP) are a promising alternative to conventional antibiotics given their broad-spectrum of activity, low propensity to induce bacterial resistance, and ability to modulate host immune responses. Dhva...
Exploring innovative adhesive approaches to manage medical adhesive-related ski...
Fialho, L; Albuquerque, J; Pinho, AS; Pereira, AM; Monteiro, C; Oliveira, N; Ferreira, S; Martins, MCLMedical adhesives are essential to secure wound care dressings and medical devices to the skin, to bind wound edges, track vital signs, or even provide local drug delivery. Despite several options in the market, most medical adhesives are composed of acrylate, hydrocolloid, and silicone, materials that are associated with the development of Medical Adhesive-Related Skin Injury (MARSI). Moreover, these injuries ...
Norbornene-chitosan nanoparticles with and without a conjugated VEGF-peptide an...
Alves, PM; Fonseca, DR; Bidarra, SJ; Gomes, A; Gomes, P; Barrias, CC; Martins, MCLImpaired angiogenesis in skin chronic wounds prolongs inflammation and compromises wound healing. Several strategies have been attempted to improve vascularization, such as application of growth factors, like the vascular endothelial growth factor (VEGF). However, VEGF is expensive, has a short half-life in vivo and has been associated to tumorigenesis when used in high concentrations. QK peptide is a shorter (...
Effect of NaOH etching and oxygen plasma treatments on surface characteristics ...
Costa, NA; Ribeiro, AR; Rossi, AL; Monteiro, C; Lisboa, PN; Martins, MCLActivation treatments such as NaOH etching or O2 plasma can play an essential role in surface conjugation of titanium with biomolecules, providing a better interaction at the bone-implant interface. However, their application on complex titanium dioxide (TiO2) surfaces is still not explored. In this contribution, bioactive and porous TiO2 coatings produced by micro-arc oxidation (MAO) were treated with NaOH etc...