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Unveiling a Listeria monocytogenes Outbreak in a Rabbit Farm: Clinical Manifest...

Rodrigues, Inês C.; Ribeiro-Almeida, Marisa; Silveira, Leonor; Prata, Joana C.; de Carvalho, André Pinto; Roque, Carla; Gomes, João Paulo; Borges, Vítor

Listeria monocytogenes poses a threat to both human and animal health. This work describes an L. monocytogenes outbreak in a Portuguese rabbit farm, detailing the isolates’ clinical manifestations, necropsy findings, and phenotypic and genomic profiles. Clinical signs, exclusively observed in does, included lethargy and reproductive signs. Post-mortem examination of does revealed splenomegaly, hepatomegaly with...


Unlocking success: the crucial role of good clinical practices in veterinary pr...

Rodrigues, Inês C.; Ribeiro-Almeida, Marisa; Ribeiro, Jorge; Silveira, Leonor; C. Prata, Joana; Pista, Ângela; Martins da Costa, Paulo

Background: Antimicrobial resistance (AMR) poses a global health challenge, impacting both humans and animals [1]. The emergence and spread of antibiotic-resistant bacteria among pets heighten the risk of transmission to humans or animals, given their close interaction [2]. Objective: This study aimed to underscore the importance of implementing good clinical practices in veterinary medicine, using a clini...

Date: 2024   |   Origin: Scientific Letters

Gulls in Porto Coastline as Reservoirs for Salmonella spp.: Findings from 2008 ...

Rodrigues, Inês C.; Cristal, Ana Paula; Ribeiro-Almeida, Marisa; Silveira, Leonor; Prata, Joana C.; Simões, Roméo; Vaz-Pires, Paulo; Pista, Ângela

Gulls act as intermediaries in the exchange of microorganisms between the environment and human settlements, including Salmonella spp. This study assessed the antimicrobial resistance and molecular profiles of Salmonella spp. isolates obtained from fecal samples of gulls in the city of Porto, Portugal, in 2008 and 2023 and from water samples in 2023. Antimicrobial susceptibility profiling revealed an improvemen...


Occurrence of Multidrug-Resistant Bacteria Resulting from the Selective Pressur...

Rodrigues, Inês C.; Ribeiro-Almeida, Marisa; Ribeiro, Jorge; Silveira, Leonor; Prata, Joana C.; Pista, Angela; Martins da Costa, Paulo

Because of public health concerns, much greater scrutiny is now placed on antibiotic use in pets, especially for antimicrobial agents that have human analogs. Therefore, this study aimed to characterize the phenotypic and genotypic profiles of multidrug-resistant bacteria isolated from nasal swabs samples taken from a one-year-old male Serra da Estrela dog with rhinorrhea that was treated with amikacin. An exte...


Caracterização genotípica e fenotípica de estirpes de Escherichia coli patogéni...

Batista, Rita; Saraiva, Margarida; Lopes, Teresa; Silveira, Leonor; Coelho, Anabela; Furtado, Rosália; Castro, Rita; Correia, Cristina Belo

As aves são potenciais portadoras de microrganismos patogénicos que afetam os seres humanos, e podem ser disseminadoras de perigos no ambiente de produção primária de géneros alimentícios de origem vege- tal e animal. Este trabalho teve como objetivo avaliar a ocorrência, em fezes de aves em liberdade, em Portugal, de três bactérias zoonóticas causadoras de infeções no Homem. Para tal, foi avaliada a presença d...


High diversity of pathogenic Escherichia coli clones carrying mcr‐1 among gulls...

Ribeiro‐Almeida, Marisa; Mourão, Joana; Novais, Ângela; Pereira, Sofia; Freitas‐Silva, Joana; Ribeiro, Sofia; Martins da Costa, Paulo; Peixe, Luísa

The expansion of mcr-carrying bacteria is a well-recognized public health problem. Measures to contain mcr spread have mainly been focused on the food-animal production sector. Nevertheless, the spread of MCR producers at the environmental interface particularly driven by the increasing population of gulls in coastal cities has been less explored. Occurrence of mcr-carrying Escherichia coli in gull's colonies f...


Effect of immunocastration and caponization on fatty acid composition of male c...

Antunes, Irene; Quaresma, M.A.G.; Ribeiro, Maria; Alves, S.P.; Martins da Costa, Paulo; Bessa, R.J.B.

Recently, immunocastration with Improvac (ImmC). has been tested in broilers and a considerable reduction in serum testosterone concentration (reduced by 79% compared to roosters) was observed. The aim of this study was to evaluate the effect of immunocastration on meat fatty acid (FA) composition and its comparison with caponized and intact males (roosters). The study was conducted with 3 experimental groups: ...


Chemical Composition, Antibacterial, Antibiofilm and Synergistic Properties of ...

Vieira, Maria; Bessa, Lucinda; Martins, M. Rosario; Arantes, Silvia; Teixeira, António; Mendes, Angelo; Martins da Costa, Paulo; Belo, A.D.F.

Essential oils (EOs) from Eucalyptus globulus LABILL. ssp. globulus and from Mediterranean autochthonous aromatic plants – Thymus mastichina L., Mentha pulegium L., Rosmarinus officinalis L., Calamintha nepeta (L.) SAVI ssp. nepeta, Cistus ladanifer L., Foeniculum vulgare L., Dittrichia viscosa (L.) GREUTER ssp. viscosa – were extracted by hydrodistillation and characterized by GC-FID and NMR spectroscopy. EOs ...


Immunocastration as an alternative to caponization: evaluation of its effect on...

Quaresma, M.A.G.; Antunes, I.C.; Ribeiro, Maria; Prazeres, S; Bessa, R.J.B.; Martins da Costa, Paulo

Caponization is associated with some morbidity and mortality, which contributes to important economic losses. This practice is executed without any pain relief (neither anesthesia nor analgesia) and can be painful and without consideration of animal welfare. On the other hand, immunocastration accomplished by Improvac and Bovipriva in pigs and cattle represents a noninvasive procedure, and for that reason is re...


Neofiscalin A and fiscalin C are potential novel indole alkaloid alternatives f...

Bessa, Lucinda J.; Buttachon, Suradet; Dethoup, Tida; Martins, Rosario; Vasconcelos, Vitor; Kijjoa, Anake; Martins da Costa, Paulo

Ten indole alkaloids were obtained from the marine sponge-associated fungus Neosartorya siamensis KUFA 0017. We studied the antimicrobial properties of these and of three other compounds previously isolated from the soil fungus N. siamensis KUFC 6349. Only neofiscalin A showed antimicrobial activity against Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resis...


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