3 documents found, page 1 of 1
Effects of ionizing radiation on proteins in lyophilized or frozen demineralize...
Antebi,Uri; Mathor,Monica Beatriz; Silva,André Ferreira da; Guimarães,Rodrigo Pereira; Honda,Emerson KiyoshiOBJECTIVE: The aim was to study the effects of application of ionizing radiation (gamma and electrons) as sterilizing agents at doses of 15 kGy, 25 kGy and 50 kGy, on lyophilized or frozen demineralized bone tissue for use in transplants. METHODS: Five human femoral diaphyses from different donors of musculoskeletal tissue were demineralized and preserved as lyophilized or frozen at -80 °C. The samples were div...
Evaluation of microRNA expression in head and neck squamous cell carcinoma cell...
Andreghetto,Flavia Maziero; Klingbeil,Maria Fatima Guarizo; Soares,Renata Machado; Sitnik,Roberta; Pinto Junior,Décio dos Santos; Mathor,Monica BeatrizABSTRACT Objective: Functional in vitro studies are fundamental to understand the role of microRNAs, small non coding RNA molecules that function as post-transcriptional regulators, in cancer. The objective of this study was to determine the applicability of head and neck squamous cell carcinoma cell lines and human oral keratinocytes as models for functional studies on microRNAs previously identified as deregu...
Ultrastructural evaluation of human keratinocyte growth and differentiation on ...
Tanikawa,Daniela Yukie Sakai; Alonso,Nivaldo; Herson,Marisa Roma; Mathor,Monica Beatriz; Caldini,Elia Garcia; Lourenço,Silvia VanessaPURPOSE: In order to circumvent several difficulties that have been met in the routine use of the in vitro keratinocyte cultures using the standard procedure described by Rheinwald and Green, and obtain a more resilient and the least possible immunogeneic skin substitute for a future clinical application, this work studied a new keratinocyte culture system, which envisages the utilization of a fibrin substrate ...