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Epidemiological Data and Antimicrobial Resistance of Campylobacter spp. in Port...

Duarte, Andreia; Pereira, Luísa; Lemos, Maria-Leonor; Pinto, Miguel; Rodrigues, João Carlos; Matias, Rui; Santos, Andrea; PTCampyNet; Oleastro, Mónica

This study extensively analyzed campylobacteriosis surveillance in Portugal from 2009 to 2021, aiming to investigate demographic shifts, seasonal variations, and antimicrobial resistance (AMR) within Campylobacter isolates. Surveillance network and sentinel laboratory-based system data revealed a substantial under-notification of campylobacteriosis cases, suggesting an underestimated disease burden. Notificatio...


Lagos ornamentais como reservatórios de microrganismos resistentes a antimicrob...

Rodrigues, João; Matias, Rui; Jordão, Luísa

Os espaços verdes nas grandes cidades têm ganho uma importância crescente para a qualidade de vida e saúde da população. Os lagos e fontes ornamentais presentes nestes espaços são muito atrativos, mas podem esconder alguns perigos. Neste estudo foi avaliada mensalmente durante um ano a flora bacteriana (planctónica e organizada em biofilmes) presente num lago ornamental de um parque urbano situado em Lisboa (Po...


Aeromonas spp. in Freshwater Bodies: Antimicrobial Resistance and Biofilm Assembly

Nascimento, Maria; Rodrigues, João; Matias, Rui; Jordão, Luisa

Aeromonas spp. are environmental bacteria able to infect animals and humans. Here, we aim to evaluate the role of biofilms in Aeromonas persistence in freshwater. Aeromonas were isolated from water and biofilm samples and identified by Vitek-MS and 16S rRNA sequencing. Antibiotic susceptibility profiles were determined according to EUCAST, and a crystal violet assay was used to assess biofilm assembly. MTT and ...


Occurrence of persistent organic pollutants at Alqueva's surface water at touri...

Raposo, Ana; Mansilha, Catarina; Veber, Alexander; Melo, Armindo; Rodrigues, Joao; Matias, Rui; Rebelo, Helena; Grossinho, José; Cano, Manuela

Freshwater pollution is a huge concern. A study aiming to evaluate water quality and occurence of two groups of persistent environmental pollutants with similar chemical properties (polycyclic aromatic hydrocarbons-PAHs and microplastics -MPs) in Alqueva’s surface water was performed during 2021. Samples were collected at three spots related to touristic activities (two beaches and one marina) once by season. I...


Occurrence of polycyclic aromatic hydrocarbons, microplastics and biofilms in A...

Raposo, Ana; Mansilha, Catarina; Veber, Alexander; Melo, Armindo; Rodrigues, Joao; Matias, Rui; Rebelo, Helena; Grossinho, José; Cano, Manuela

Freshwater pollution is a huge concern. A study aiming to evaluate physico-chemical characteristics, microbiota, occurrence of two groups of persistent environmental pollutants with similar chemical properties (polycyclic aromatic hydrocarbons- PAHs and microplastics - MPs) in Alqueva's surface water was performed during 2021. Water samples were collected at three spots related to touristic activities (two beac...


Assessment of the Transmission Dynamics of Clostridioides difficile in a Farm E...

Alves, Frederico; Nunes, Alexandra; Castro, Rita; Sequeira, António; Moreira, Olga; Matias, Rui; Rodrigues, João Carlos; Silveira, Leonor

The recent increase in community-acquired Clostridioides difficile infections discloses the shift in this bacterium epidemiology. This study aimed at establishing a transmission network involving One Health components, as well as assessing the zoonotic potential and genomic features of dominant clones. Samples were collected from different compartments of animal, human and environmental origin, from an animal p...


Prospective observational study on the role of catheter colonization and multid...

Pinto, Miguel; Borges, Vitor; Nascimento, Maria; Martins, Filomena; Pessanha, Maria Ana; Faria, Isabel; Rodrigues, Joao; Matias, Rui; Joao Paulo, Gomes

Background: Central venous catheter (CVC)-related bloodstream infection (CRBSI) is a huge public health consern with considerable impact on mortality and health costs. The emergence of antimicrobial resistant microorganisms associated or not with CVC colonization by biofilms makes the treatment of CRBSI even more challeging. Methods:A 3-year observational study enrolling 3 tertiary hospitals located in Lisbon (...


Catheter related bloodstream infection caused by E. cloacae and Candida parapsi...

Štefánek, Matúš; Borges, Vítor; Wenner, Sigurd; Nogueira, Isabel D.; Pinto, Miguel; Faria, Isabel; Pessanha, Maria Ana; Veríssimo, Cristina

Biofilm-associated infections is a public health concern in the context of healthcare associated infections (HAI) such as catheter related bloodstream infections (CRBSI). Here the dynamics of two top ten etiological agents of CRBSI, Enterobacter cloacae and Candida parapsilosis isolated from a CRBSI’s patient, were studied to get insights on the role played by biofilms on this HAI. Antimicrobial susceptibility ...


Insights on catheter-related bloodstream infections: a prospective observationa...

Pinto, Miguel; Borges, Vítor; Nascimento, Maria; Martins, Filomena; Pessanha, Maria Ana; Faria, Isabel; Rodrigues, João; Matias, Rui; Gomes, João Paulo

Background: Central venous catheter-related bloodstream infection (CRBSI) is a huge public health concern with considerable impact on mortality and health costs. Aim: A three-year observational study enrolling three tertiary hospitals located in Lisbon, Portugal, was designed to identify the major aetiological agents of CRBSI, their ability to colonize central venous catheters and their antimicrobial resistance...


Diversity of bacterial communities and genes encoding AMR in different environm...

Manageiro, Vera; Cano, Manuela; Dias, Elsa; Rosado, Tânia; Vieira, Patrícia; Reis, Lígia; Matias, Rui; Rodrigues, João; Coelho, Rodrigo; Menezes, Carina

Aim: The aim of this study was to determine the microbial biodiversity and naturally occurring antibiotic resistance (AR)-encoding genes background load along the food/feed chain in an open-air agricultural testing catchment (HOAL). Methods: Samples were collected in two time points during the year 2020 from various – but interconnected – environmental compartments of the food/feed chain within an HOAL catchmen...


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