6 documents found, page 1 of 1
Extract of Amburana cearensis maintains the survival of ovine preantral follicl...
Menezes, Vanúzia Gonçalves; Barberino, Ricássio de Sousa; Gouveia, Bruna Bortoloni; Gonçalves, Rodrigo José de SouzaThis study evaluated the effect of Amburana cearensis extract as a preservation or culture medium for ovine ovarian tissue. Ovarian fragments were fixed in 4% buffered formaldehyde for 18 h (fresh control), stored in Minimal Essential Medium (MEM) or in A. cearensis extract (0.1; 0.2 or 0.4 mg/mL) at a temperature of 4ºC for 6, 12 or 24 h (preservation - experiment 1) or cultured for 7 days in ?-MEM+ or in A. c...
Progesterone and follicle stimulating hormone interact and promote goat preantr...
Lima-Verde, Isabel Bezerra; Matos, Maria Helena Tavares de; Celestino, Juliana Jales de Hollanda; Rossetto, Rafael; Name, Khesller Patrícia OláziaEste trabalho veri_icou os efeitos da progesterona e do hormonio foliculo-estimulante (FSH) na sobrevivencia e no crescimento de foliculos pre-antrais caprinos. Fragmentos de tecido ovariano foram cultivados por 1 ou 7 dias em Meio Essencial Minimo (MEM) sozinho ou contendo progesterona (1, 2.5, 5, 10 ou 20ng/mL), FSH (50ng/mL) ou a combinacao entre esses dois hormonios. O tecido fresco (controle nao-cultivado)...
Cryopreservation of caprine ovarian tissue using glycerol and ethylene glycol
Rodrigues, Ana Paula Ribeiro; Amorim, C.A; Costa, Sonia Helena Furtado; Matos, Maria Helena Tavares de; Santos, R.R.; Lucci, Carolina MadeiraCryopreservation of ovarian tissue may be a potential alternative for the conservation of genetically superior animals, including high milk- and meat-producing goat breeds. However, until now, no information was available concerning the cryopreservation of preantral follicles (PF) enclosed in caprine ovarian tissue. The objective of the present study was to evaluate the structural and ultrastructural characteri...
Morphological and ultrastructural analysis of sheep primordial follicles preser...
Matos, Maria Helena Tavares de; Andrade, Evelyn Rabelo; Lucci, Carolina Madeira; Báo, Sônia Nair; Silva, José Roberto Viana; Santos, R.R.The objective was to determine the morphological and ultrastructural features of sheep primordial follicles preserved in either 0.9% saline solution or TCM 199 at different temperatures. Soon after death, the ovarian pair of each ewe (n=5) was divided into 25 fragments. One fragment was immediately fixed for morphological evaluation (control). The other 24 fragments were randomly distributed in tubes containing...
Estudo morfológico e morfométrico da próstata de cobaios (Cavia porcellus, Linn...
Gradela, Adriana; Nunes, Amanda Karoline Rodrigues; Martins, Laura Flávia Teixeira; Santos, Juliana Muniz dos; Matos, Maria Helena Tavares deA próstata é muito importante clinicamente, pois desempenha um papel importante na produção de sêmen e fertilização do óvulo, além de ser frequentemente acometida por doenças como a hiperplasia prostática e o câncer de próstata. O objetivo deste estudo foi avaliar a morfologia, morfometria, índice organossomático (IOS), espessura da parede e altura do epitélio da próstata de cobaios em diferentes fases do desen...
Different Follicle-Stimulating Hormone (FSH) sources influence caprine preantra...
Magalhães, Deborah de Melo; Araujo, Valdevane Rocha; Verde, Isabel Bezerra Lima; Matos, Maria Helena Tavares de; Silva, Renata CarvalhoThe aim of this study was to evaluate the effects of pituitary (pFSH) or recombinant (rFSH) FSH on the survival and growth of caprine preantral follicles. Caprine ovarian tissues were in vitro cultured for one or seven days in Minimum Essential Medium (MEM) alone or containing 10, 50, 100 and 1000 ng/ml of pFSH or rFSH. Control tissues (non-cultured) and those cultured were processed for histological and ultras...