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Epidemiology and clinical presentation of community-acquired Staphylococcus aur...

Staphylococcus aureus bacteraemia (SAB) is one of the most common bloodstream infections globally. Data on the burden and epidemiology of community-acquired SAB in low-income countries are scarce but needed to defne preventive and management strategies. Blood samples were collected from children<5 years of age with fever or severe disease admitted to the Manhiça District Hospital for bacterial isolation, includ...

Full survival of Galleria mellonella infected with Staphylococcus aureus after ...

Diabetes mellitus affects nearly 6.4% of the worldwide population, and this number may double by 2030. Up to 25% of diabetic patients may develop diabetic foot ulcers (DFUs). Among DFU patients, 80% will suffer lower-limb amputations due to diabetic foot infections (DFIs), which are generally colonized by polymicrobial biofilms. Staphylococcus aureus is the DFIs’ predominant pathogen, frequently found together ...

Plasmid profiling of Staphylococcus pseudintermedius associated with skin and s...

Assessment of chlorohexidine and benzalkonium chloride activity against Staphyl...

Introduction: Staphylococcus pseudintermedius is the main bacterial pathogen responsible for skin and soft-tissue infections (SSTIs) in companion animals and is increasingly associated with antimicrobial resistance (AMR). Objective: This study analyzed the effectiveness of the biocides chlorhexidine (CHX) and benzalkonium chloride (BAC) against S. pseudintermedius causing SSTIs in companion animals. Methodology...

Complete sequences of two Staphylococcus aureus plasmids carrying genes for res...

[P292]; Aim: To gain detailed insight into the genetic organization of two multidrug resistance plasmids, pSM31 and pSM39, from clinical S. aureus isolates collected in a hospital in Lisbon, Portugal, and to identify factors that may explain their selection and persistence. Methods: Plasmid pSM31 was sequenced by Sanger sequencing using cloning and primer walking whereas pSM39 was sequenced by next generation s...

Frequent biofilm production by Staphylococcus epidermidis causing infection in ...

[P270]; Aim: To characterize biofilm production by Staphylococcus epidermidis isolates causing infection in pets or colonizing veterinary staff. Methods: The study collection comprised 129 S. epidermidis isolates, from nasal colonization of vet-erinary staff (n = 112) and causing infection in cats and dogs (n = 17). The capacity to produce biofilm was evaluated by the Crystal Violet (CV) method, performed in fl...

Emergence of non-wild-type populations towards biocides in Staphylococcus epide...

[P269]; Aims: To characterize biocide susceptibility of Staphylococcus epidermidis colonizing veterinary staff; propose epidemiological cut-off (ECOFF) values; assess relationships between biocide and antibiotic resistance phenotypes.Methods: The susceptibility profiles of 112 S. epidermidis isolates from nasal colonization of veter-inary staff towards six biocides (including benzalkonium chloride, chlorhexidin...

Biocide susceptibility in Staphylococcus epidermidis causing infection in pets

[P280]; Aim: To characterize biocide susceptibility of Staphylococcus epidermidis isolates causing infection in pets and evaluate proposed biocide epidemiological cut-off (tentative ECOFF) values. Methods: The study comprised a collection of 17 S. epidermidis isolates collected from several infection sources in cats and dogs from 2001 to 2016. Susceptibility to benzalkonium chloride (BAC), cetrim-ide, chlorhexi...

Development of multidrug resistance in staphylococci driven by efflux

[P282]; Aim: To study the efflux driven response of two major staphylococcal pathogens, S. aureus and S. epi-dermidis to the challenge by non-antibiotic drugs. Methods: We adapted three reference strains to ethidium bromide (EtBr), a broad substrate of bacte-rial efflux pumps. The parental strains, S. aureus ATCC25923, S. epidermidis ATCC12228 and S. epider-midis RP62A were cultured in varying concentrations of...

Characterization of antimicrobial resistance in Staphylococcus epidermidis colo...