Quality management and independent assessment of high-throughput sequencing-based virus diagnostics have not yet been established as a mandatory approach for ensuring comparable results. The sensitivity and specificity of viral high-throughput sequence data analysis are highly affected by bioinformatics processing using publicly available and custom tools and databases and thus differ widely between individuals...
Program of Research Excellence (PROEP ? IOC/Fiocruz/CNPq), the National Council for Scientific and Technological Development (CNPq), project PAPES VI/FIOCRUZ ? CNPq, Oswaldo Cruz Institute (IOC/FIOCRUZ), Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES) ? project CAPES-MERCOSUL PPCP 023/2011, the General Coordination of Public Health Laboratories ? Secretary of Health Surveillanc...
We conducted a viral metagenomics study in diarrheic free-ranging wolves in Portugal, revealing for the first time the presence of reassortant picobirnaviruses. These viruses shared identical capsid segments together with diverse RNA-dependent RNA polymerase segments. Even though causality between these picobirnaviruses and diarrhea could not be established, the study nonetheless confirms for the first time tha...
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