26 documents found, page 1 of 3

Immunochemotherapy in american cutaneous leishmaniasis : immunological aspects ...

In this study, we evaluated the immune response of patients suffering from cutaneous leishmaniasis treated with two distinct protocols. One group was treated with conventional chemotherapy using pentavalent antimonium salts and the other with immunochemotherapy where a vaccine against cutaneous leishmaniasis was combined with the antimonium salt. Our results show that, although no differences were observed in t...

Systemic and compartmentalized immune response in canine visceral leishmaniasis.

Human visceral leishmaniasis (VL) and canine visceral leishmaniasis (CVL) are the most important emerging diseases with high prevalence in Latin American countries and are mainly caused by Leishmania (L.) chagasi (Syn = L. infantum). CVL has a great impact on Brazilian public health because domestic dogs are the most important VL peri-domicile reservoirs in both urban and peri-urban areas. Our findings highligh...

Vaccine for prophylaxis and immunotherapy, Brazil.

Vaccination of C57BL/10 mice against cutaneous leishmaniasis using killed proma...

Antigenic extracts from five Leishmania stocks were used to vaccinate C57BL/10 mice. The Leishvacinand PH8 monovalent vaccine yielded the highest IFN-levels in the supernatants of spleen cell culture from vaccinated animals. Each single strain immunized group showed evidence of protective immunity six months after the challenge with promastigotes of Leishmania (Leishmania) amazonensis. No differences were d...

Immunotherapy, immunochemotherapy and chemotherapy for American cutaneous leish...

O tratamento de primeira escolha para leishmaniose tegumentar americana é o antimonial pentavalente. Embora este tratamento seja na maioria das vezes efetivo e indicado, devem ser consideradas as desvantagens tais como efeitos colaterais, longa duração do tratamento e contra-indicação para cardiopatas, nefropatas, idosos, grávidas e outras condições. Com o advento da vacina antileishmaniose tegumentar americana...

Comparative evaluation of phenol and thimerosal as preservatives for a candidat...

For decades thimerosal has been used as a preservative in the candidate vaccine for cutaneous leishmaniasis, which was developed by Mayrink et al. The use of thimerosal in humans has been banned due to its mercury content. This study addresses the standardization of phenol as a new candidate vaccine preservative. We have found that the proteolytic activity was abolished when the test was conducted using the can...

Comparison of polymerase chain reaction with other laboratory methods for the d...

An evaluation of 5 laboratory methods for diagnosing American cutaneous leishmaniasis (ACL) was carried out on patients from an endemic area of Brazil. From 164 patients presenting cutaneous lesions, and suspected to have ACL, 133 (81.1%) were confirmed for the disease by Montenegro skin test (MST) and/or parasitologic examination (PE). In both groups of patients, the positivity of polymerase chain reaction (PC...

Epidemiology of canine visceral leishmaniasis in the endemic area of Montes Cla...

The Montes Claros City is located in an endemic area for visceral leishmaniosis in the Minas Gerais State, Brazil.With the implementation of a program for the control of visceral leishmaniosis in 1994, a sectional study was carried out to evaluate the infection by viscerotropic Leishmania in the population of dogs from Montes Claros, basically using indirect immunofluorescence antibody test (IFAT). Blood sample...

Leishmaniose visceral canina : avaliação da metodologia sorológica utilizada em...

Foi realizado um estudo comparativo da reação de imunofluorescencia indireta em eluatos de sangue de cães infectados experimentalmente com diferentes tripanosomatideos. Utilizaram-se como antigenopromastigotas de L. mexicana, L. braziliensis e L. chagasi. Os resultados mostraram que a sensibilidade do método foi de 87,5% para o diagnostico do calazar canino, independentemente do antígeno empregado; e que ocorre...

Comparison among three polymerase chain reaction assays on detection of DNA fro...

Introdução: Analisou-se a positividade da reação em cadeia da polimerase (PCR) na detecção de DNA de Leishmania em pacientes. Métodos: DNA extraído foi submetido a L150/L152, 13Y/13Z e PCR seminested (snPCR). Resultados: Resultados foram evidenciados por bandas de aproximadamente 120; 720 e 670pb para L150/L152, 13Y/13Z e snPCR, respectivamente. Positividades para L150/L152, 13Y/13Z e snPCR foram 76,9; 56,4 e 6...