37 documents found, page 1 of 4
Sugarcane cell suspension reveals major metabolic changes under different nitro...
Bottcher,Alexandra; Domingues-Junior,Adilson Pereira; Souza,Leonardo Perez de; Tohge,Takayuki; Araújo,Wagner Luiz; Fernie,Alisdair RobertABSTRACT Cell suspension culture has been used as a model to study metabolic changes to several stresses. To have detailed information of nitrogen (N) limitation on sugarcane metabolism, a controlled study of the primary metabolites and representative compounds of secondary metabolism was developed using suspension cells growing under three different N regimes: normal condition (40 mmol·L–1 NO-3), slightly defi...
Eucalyptus urograndis stem proteome is responsive to short-term cold stress
Leonardi,Gabriela de Almeida; Carlos,Natália Aparecida; Mazzafera,Paulo; Balbuena,Tiago SantanaEucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensiona...
Aquaporins and the control of the water status in coffee plants
Santos,Adriana Brombini dos; Mazzafera,PauloSix aquaporin (AQP) homologues were identified in three coffee species (Coffea arabica, C. Canephora, and C. racemosa), including four plasma membrane intrinsic proteins (PIP1;1, PIP1;2, PIP2;1, and PIP2;2) and two tonoplast intrinsic proteins (TIP1;1 and TIP1;2). In order to better understand the role of these genes in coffee, C. arabica cvs Catuaí and Mundo Novo, C. canephora cv. Apoatã, and a graft of Mundo ...
Simple procedure for nutrient analysis of coffee plant with energy dispersive X...
Tezotto,Tiago; Favarin,José Laércio; Paula Neto,Ana; Gratão,Priscila Lupino; Azevedo,Ricardo Antunes; Mazzafera,PauloNutrient analysis is used to estimate nutrient content of crop plants to manage fertilizer application for sustained crop production. Direct solid analysis of agricultural and environmental samples by energy dispersive X-ray fluorescence spectrometry (EDXRF) was chosen as alternative technique to evaluate the simultaneous multielemental quantification of the most important essential elements in coffee (Coffea a...
An overview of lignin metabolism and its effect on biomass recalcitrance
Cesarino,Igor; Araújo,Pedro; Domingues Júnior,Adilson Pereira; Mazzafera,PauloLignin, after cellulose, is the second most abundant biopolymer on Earth, accounting for 30% of the organic carbon in the biosphere. It is considered an important evolutionary adaptation of plants during their transition from the aquatic environment to land, since it bestowed the early tracheophytes with physical support to stand upright and enabled long-distance transport of water and solutes by waterproofing ...
Influence of air temperature on proteinase activity and beverage quality in Cof...
Abreu,Hellen Marília Couto de; Nobile,Paula Macedo; Shimizu,Milton Massao; Yamamoto,Paula Yuri; Silva,Emerson Alves; Colombo,Carlos AugustoFruits were collected from trees of Coffea arabica cv. Obatã grown at Mococa and Adamantina in São Paulo State, Brazil, which are regions with marked differences in air temperature that produce coffee with distinct qualities. Mococa is a cooler location that produces high-quality coffee, whereas coffee from Adamantina is of lower quality. The amino acid and protein contents, amino acid profile, and proteinase a...
Characterisation of AC1: a naturally decaffeinated coffee
Benatti,Luciana Benjamim; Silvarolla,Maria Bernadete; Mazzafera,PauloWe compared the biochemical characteristics of the beans of a naturally decaffeinated Arabica coffee (AC1) discovered in 2004 with those of the widely grown Brazilian Arabica cultivar "Mundo Novo" (MN). Although we observed differences during fruit development, the contents of amino acids, organic acids, chlorogenic acids, soluble sugars and trigonelline were similar in the ripe fruits of AC1 and MN. AC1 beans ...
A low cost apparatus for measuring the xylem hydraulic conductance in plants
Pereira,Luciano; Mazzafera,PauloPlant yield and resistance to drought are directly related to the efficiency of the xylem hydraulic conductance and the ability of this system to avoid interrupting the flow of water. In this paper we described in detail the assembling of an apparatus proposed by TYREE et al. (2002), and its calibration, as well as low cost adaptations that make the equipment accessible for everyone working in this research are...
Antioxidative responses of cell suspension cultures of two Coffea arabica varie...
Bottcher,Alexandra; Nobile,Paula Macedo; Martins,Paula Fabiane; Conte,Fábio Frangiotti; Azevedo,Ricardo Antunes; Mazzafera,PauloThe effects of aluminum (Al) on the activities of antioxidant enzymes and ferritin expression were studied in cell suspension cultures of two varieties of Coffea arabica, Mundo Novo and Icatu, in medium with pH at 5.8. The cells were incubated with 300 µM Al3+, and the Al speciation as Al3+ was 1.45% of the mole fraction. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferas...
Efeito da carga pendente na qualidade de frutos de laranjeira 'Valência'
Duarte,Thiago Franco; Bron,Ilana Urbano; Ribeiro,Rafael Vasconcelos; Machado,Eduardo Caruso; Mazzafera,Paulo; Shimizu,Miton MassaoAlgumas espécies de citros apresentam produção irregular ao longo dos anos. Além da safra irregular, a alternância de produção pode interferir na qualidade dos frutos, característica cada vez mais valorizada pelo mercado citrícola. O objetivo do trabalho foi avaliar o efeito da carga pendente na qualidade dos frutos da laranjeira 'Valência'. O experimento foi conduzido com laranjeiras 'Valência' enxertadas em t...