12 documents found, page 1 of 2
Detection and molecular characterization of Cryptosporidium spp. in captive can...
Camargo,Vinícius da Silva; Santana,Bruna Nicoleti; Ferrari,Elis Domingos; Nakamura,Alex Akira; Nagata,Walter Bertequini; Nardi,Ana Rita MoraesAbstract This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-flotation using Sheather's solution. Cryptosporidium spp. diagnosis was performed using three diagnostic methods: malachite green negative staining, n...
Evaluation of recombinant Cryptosporidium hominis GP60 protein and anti-GP60 ch...
Miura,Valéria Chamas; Aoki,Sérgio Moraes; Peitl Junior,Paulo; Pires,Lilian Campos; Dalmagro,Priscila; Nakamura,Alex Akira; Meireles,Marcelo VasconcelosAbstract In this study, a method for expressing Cryptosporidium hominis GP60 glycoprotein in Escherichia coli for production of polyclonal anti-GP60 IgY in chickens was developed aiming future studies concerning the diagnosis, prevention and treatment of cryptosporidiosis. The full-length nucleotide sequence of the C. hominis gp60 gene was codon-optimized for expression in E. coli and was synthesized in pET28-a...
Mello and Campos (1974) method adapted for the recovery of cestodes in birds ( ...
Silva,Giane Serafim da; Romera,Daiane Mompean; Prado,Maykon Bichoffi do; Soares,Vando Edésio; Meireles,Marcelo VasconcelosABSTRACT: The specific diagnosis and evaluation of the intensity of avian helminth infections are essential for efficacy studies and the determination of drug doses targeted to their control. This study evaluated the Mello and Campos method, originally described for parasitological diagnosis in dogs, in the recovery of scolices from cestode parasites of poultry (Gallus domesticus ). A total of 52 naturally infe...
Cryptosporidium infections in birds - a review
Nakamura,Alex Akira; Meireles,Marcelo VasconcelosCryptosporidiosis is one of the main protozoan infections in birds. It manifests as either a respiratory or a digestive illness, and it affects a very large number of avian species across several continents. The aim of this review is to report on the main results of studies on cryptosporidiosis among birds and the importance of these results to veterinary medicine and public health.
Ocorrência da infecção por Cryptosporidium spp. em cabritos (Capra hircus)
Brito,Roberta Lomonte Lemos de; Inácio,Sandra Valéria; Oliveira,Dalilian Antoniete dos Santos; Sousa,Maximiana Mesquita de; Meireles,Marcelo VasconcelosO presente estudo teve como objetivo determinar a ocorrência da infecção por Cryptosporidium spp. em cabritos de Quixadá, Ceará, Brasil. Participaram do estudo 400 cabritos, com idade entre três e 360 dias, de ambos os sexos, com e sem padrão racial definido, procedentes de 25 estabelecimentos rurais distribuídos em três circuitos. As fezes foram cadastradas de acordo com o aspecto e cor, distribuídas em tubos ...
Pesquisa de oocistos de Isospora spp. em passeriformes criados em cativeiro
Silva,Deuvânia Carvalho da; Homem,Camila Guariz; Nakamura,Alex Akira; Silva,Valéria Cristina da; Meireles,Marcelo VasconcelosO presente estudo teve como objetivo pesquisar, em longo prazo, a presença de oocistos de Isospora spp. em várias espécies de passeriformes, naturalmente infectadas, criadas em cativeiro. Foram colhidas 289 amostras em dois criatórios de passeriformes, onde houve comprovação prévia de infecção por Isospora, nos quais havia alojamento de várias espécies de passeriformes adultos. As amostras foram colhidas de for...
Cryptosporidium spp. infection in mares and foals of the northwest region of Sã...
Inácio,Sandra Valéria; Brito,Roberta Lomonte Lemos de; Zucatto,Anaiza Simão; Coelho,Willian Marinho Dourado; Aquino,Monally Conceição Costa deThe present study aimed to analyze the occurrence of infection by Cryptosporidium spp. in mares and their respective foals. This study was carried out in 11 farms located in the municipalities of Araçatuba, Birigui, Guararapes and Santo Antônio do Aracangua, in the northwest region of the State of Sao Paulo, from November 2010 to March 2011. A total of 98 mares and 98 foals of several breeds were analyzed; amon...
Diagnóstico de criptosporidiose em amostras fecais de bezerros por imunofluores...
Teixeira,Weslen Fabricio Pires; Coelho,William Marinho Dourado; Soutello,Ricardo Velludo Gomes de; Oliveira,Fernando Paes de; Homem,Camila GuarizO presente estudo teve como objetivo comparar as técnicas de imunofluorescência direta (IFD) e a microscopia de contraste de fase em solução de Sheather (MCF), para detecção de oocistos de Cryptosporidium spp. em amostras fecais de bezerros. A determinação dos limiares detecção da IFD e da MCF foi realizada utilizando cinco alíquotas de uma amostra fecal de bezerro, comprovadamente negativa para Cryptosporidium...
Detection of Cryptosporidium parvum oocysts in calf fecal samples by direct imm...
Teixeira,Weslen Fabricio Pires; Coelho,Willian Marinho Dourado; Nunes,Cáris Maroni; Meireles,Marcelo VasconcelosThe aim of this study was to produce a conjugate containing anti-Cryptosporidium parvum polyclonal antibodies and standardize a Direct Immunofluorescence Assay (DIF) for detecting C. parvum oocysts in fecal samples from calves. In order to obtain anti-C. parvum polyclonal antibodies, two New Zealand rabbits were immunized with a purified solution of C. parvum oocysts and Freund's adjuvant. Purification of the i...
Cryptosporidium infection in Brazil: implications for veterinary medicine and p...
Meireles,Marcelo VasconcelosThe aim of this review paper is to report the results of cryptosporidiosis research in Brazil, mainly its occurrence in animals and implications for veterinary medicine and public health. An increasing number of papers related to Cryptosporidium spp. infection in Brazil are available at national and international literature. The main focus described in these papers is the occurrence of Cryptosporidium spp. in f...