33 documents found, page 1 of 4
Bacteriophage cocktail-mediated inhibition of Pseudomonas aeruginosa biofilm on...
Oliveira, Viviane C.; Macedo, Ana P.; Melo, Luís D. R.; Santos, Sílvio B.; Hermann, Paula Regina de Souza; Silva-Lovato, Cláudia H.Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage trea...
Identification of the first endolysin Cell Binding Domain (CBD) targeting Paeni...
Santos, Sílvio B.; Oliveira, A.; Melo, Luís D. R.; Azeredo, JoanaBacteriophage endolysins present enormous biotechnological potentials and have been successfully used to control and detect bacterial pathogens. Endolysins targeting Gram-positive bacteria are modular, displaying a cell binding (CBD) and an enzymatically active domain. The CBD of phage endolysins are recognized by their high specificity and?host affinity, characteristics that make them promising diagnostic tool...
In vitro activity of bacteriophages against planktonic and biofilm populations ...
Pires, Diana Priscila Penso; Melo, Luís D. R.The in vitro activity of bacteriophages against planktonic cultures and biofilms is commonly evaluated by culture methods. However, these methods can lead to an underestimation of total bacterial cells when they undergo different physiological states. This chapter describes the methodology used to assess the in vitro activity of bacteriophages against planktonic cultures of bacteria in different metabolic state...
Assessment of Sep1virus interaction with stationary cultures by transcriptional...
Melo, Luís D. R.; França, Angela; Brandão, Ana Catarina; Sillankorva, Sanna; Cerca, Nuno; Azeredo, JoanaThe majority of phage infection studies are performed in bacteria that are growing exponentially, although in nature, phages usually interact also with non-replicating cells. These stationary-phase cells differ from exponential cells morphologically, physiologically and metabolically. The interaction of a Sep1virus with Staphylococcus epidermidis stationary and exponential phase cells was explored. Phage SEP1 e...
Synergetic efect between phage and antibiotics as an alternative strategy to co...
Akturk, Ergun; Oliveira, Hugo; Melo, Luís D. R.; Azeredo, Joana[Excerpt] Bacterial biofilms are sessile microbial aggregates with unique community properties, showing a high degree of tolerance/resistance to disinfection by chemicals, antibiotics, and to the human immune system. The opportunistic pathogen Pseudomonas aeruginosa is one of the most frequent causes of biofilm-associated infections, causing infections extremely difficult to treat. Currently, bacteriophages (ph...
Chestnut honey and bacteriophage application to control Pseudomonas aeruginosa ...
Oliveira, A.; Sousa, Jéssica; Silva, A.; Melo, Luís D. R.; Sillankorva, SannaChronic skin wounds represent a major burn both economically and socially. Pseudomonas aeruginosa and Escherichia coli are among the most common colonizers of infected wounds and are prolific biofilm formers. Biofilms are a major problem in infections due to their increasingly difficult control and eradication, and tolerance to multiple prescribed drugs. As so, alternative methods are necessary. Bacteriophages ...
Synergistic antimicrobial interaction of honey and bacteriophage in Escherichia...
Ribeiro, Henrique; Oliveira, A.; Silva, A.; Silva, Maria Daniela; Rodrigues, Célia Fortuna; Melo, Luís D. R.; Henriques, Ana Filipa; Sillankorva, SannaWound colonization by biofilms-forming bacteria is one of the main obstacles to the treatment of chronic wounds, causing a number of biological and financial problems. Biofilms are structured communities of bacterial cells enclosed in a self-produced polymeric matrix, adhered to inert or living surfaces, blocking antibiotics and patients immune cells from reaching bacteria. Bacterio(phages), viruses infecting e...
Phages against infectious diseases
Melo, Luís D. R.; Oliveira, Hugo; Santos, Sílvio B.; Sillankorva, Sanna; Azeredo, Joana(Bacterio)phages (viruses that infect bacteria) are the most abundant entities on earth. Phages are the natural predators of bacteria and therefore have a great antibacterial potential. Immediately after their discovery, in the early twentieth century and before the antibiotic era, they were extensively used to treat infectious diseases. Now, on account of the spread of antibiotic resistance, the interest in ph...
Synergistic antimicrobial interaction between honey and phage against Escherich...
Oliveira, A.; Ribeiro, Henrique; Silva, A.; Silva, Maria Daniela; Sousa, J.; Rodrigues, Célia Fortuna; Melo, Luís D. R.; Henriques, Ana FilipaChronic wounds that take months, years or may even never heal present a major biological and financial problem on both individual patients and the broader health system. Chronic wounds afford a hostile environment of damaged tissues that allow bacterial proliferation and further wound colonization. Wound colonization by bacterial biofilms is one of the main obstacles of chronic wounds healing. Biofilms are stru...
Synergistic antimicrobial interaction between honey and phage against Escherich...
Oliveira, A.; Ribeiro, Henrique; Silva, A.; Silva, Maria Daniela; Sousa, Jéssica; Rodrigues, Célia F.; Melo, Luís D. R.; Henriques, Ana FilipaChronic wounds afford a hostile environment of damaged tissues that allow bacterial proliferation and further wound colonization. Escherichia coli is among the most common colonizers of infected wounds and it is a prolific biofilm former. Living in biofilm communities, cells are protected, become more difficult to control and eradicate, and less susceptible to antibiotic therapy. This work presents insights int...