9 documents found, page 1 of 1
Chemical composition and antibacterial activity of the essential oils of Blainv...
Pires, Andreza Maria L.; Albuquerque, Maria Rose Jane R.; Nunes, Edson P.; Melo, Vânia M. M.; Silveira, Edilberto R.; Pessoa, Otília Deusdênia L.The essential oils of Blainvellea rhomboidea (Asteraceae) were obtained by hydrodistillation and analyzed by GC/MS and GC/FID. Initially, the essential oil from the aerial parts was investigated. From the 18 identified components, 5-indanol (14.5%) followed by p-cymen-8-ol (10.1%), β-caryophyllene (9.6%), caryophyllene oxide (9.6%), limonene (8.6%), terpinolene (7.8%), and spathulenol (7.7%) were the major cons...
A novel bacterial carboxylesterase identified in a metagenome derived‑clone fro...
Araujo, Francisco J.; Hissa, Denise Cavalcante; Silva, Gabrielly O.; Antunes, André Saraiva Leão Marcelo; Nogueira, Vanessa L. R.A functional screening of 1152 clones from a plasmid library constructed with DNA extracted from Brazilian mangrove sediments revealed 3 positive clones for ester-hydrolyzing enzymes, or about one lipolytic gene per 1.2 Mb DNA, which corroborates the idea that oil-contaminated mangroves are a good source of novel microbial lipases/esterases. The partial sequence of the clone LipG7 (1179 bp) showed 30.2% of pred...
One-step immobilization and stabilization of a recombinant Enterococcus faecium...
Sousa, Marylane de; Melo, Vânia M. M.; Hissa, Denise Cavalcante; Manzo, Ricardo M.; Mammarella, Enrique J.; Antunes, André Saraiva Leão MarceloA recombinant L-arabinose isomerase from Enterococcus faecium DBFIQ E36 was immobilized onto multifunctional epoxide supports by chemical adsorption and onto a chelate-activated support via polyhistidine-tag, located on the N-terminal (N-His-L-AI) or on the C-terminal (C-His-L-AI) sequence, followed by covalent bonding between the enzyme and the support. The results were compared to reversible L-AI immobilizati...
Cyclic lipopeptide signature as fingerprinting for the screening of halotoleran...
Farias, Bárbara Cibelle Soares; Hissa, Denise Cavalcante; Nascimento, Camila Tauane Monteiro do; Oliveira, Samuel A.; Zampieri, DavilaCyclic lipopeptides (CLPs) are non-ribosomal biosurfactants produced by Bacillus species that exhibit outstanding interfacial activity. The synthesis of CLPs is under genetic and environmental influence, and representatives from different families are generally co-produced, generating isoforms that differ in chemical structure and biological activities. This study to evaluate the effect of low and high NaCl con...
Microbial epibionts of the colonial ascidians Didemnum galacteum and Cystodytes...
Oliveira, Francisca Andréa da Silva; Colares, Geórgia B.; Hissa, Denise Cavalcante; Angelim, Alysson Lira; Melo, Vânia M. M.; Lotufo, Tito M. C.Symbiosis with microorganisms has been well documented for many marine invertebrate taxa. However, knowledge of the diversity of microorganisms associated with ascidians is still limited. This study assessed the microbial epibionts of Didemnum galacteum and Cystodytes sp., two ascidian species collected from the western coast of Ceará state (Brazil), at Dois Coqueiros beach and the port of Pecém, respectively. ...
Diversity of a chlorine-resistant Bacillus population isolated from a wastewate...
Paes, Fernanda Araújo; Hissa, Denise Cavalcante; Angelim, Alysson Lira; Pinto, Natasha W.; Grangeiro, Thalles B.; Melo, Vânia M. M.This paper describes the phenotypic and genotypic diversity of a Gram-positive, aerobic bacterial population isolated from the chlorine tank of a wastewater treatment plant. A total of 12 spore- forming, rod-shaped isolates were identified using 16S rRNA gene sequencing and biochemical tests. Pairwise genetic comparisons revealed the identity among sequences obtained from isolates varied from 92.6 to 100%. Simi...
Nutritional study of two Brazilian soybean (Glycine max) cultivars differing in...
Vasconcelos, Ilka M.; Maia, Andrea A. B.; Siebra, Elisângela A.; Oliveira, José T. A.; Carvalho, Ana F. U.; Melo, Vânia M. M.; Carlini, Célia R.The research was conducted with two different recently released Brazilian soybean cultivars (Rio Balsas and Bays) to evaluate whether there is any correlation between the different levels of antinutritional and/or toxic proteins in the cultivars and their nutritive value as sources of protein for monogastric animals (rats). Furthermore, it is discussed, for the first time, the role of the dietary soyatoxin on t...
Puri®cation of a novel antibacterial and haemagglutinating protein from the pur...
Melo, Vânia M. M.; Duarte, Ana B. G.; Carvalho, Ana F. U.; Siebra, Elisângela A.; Vasconcelos, Ilka M.Physicochemical characterisation and antibacterial and haemagglutinating properties of a new protein isolated from purple fluid of the Aplysia dactylomela are reported. The purification procedure consisted basically of ammonium sulphate fractionation, ion exchange, exclusion molecular and hydrophobic interaction chromatography. The highly purified protein, designated dactylomelin-P, is a single chain protein of...
Larvicidal activity of the water extract of Moringa oleifera seeds against Aede...
Ferreira, Paulo M. P.; Carvalho, Ana F. U.; Farias, Davi F.; Cariolano, Nara G.; Melo, Vânia M. M.; Queiroz, Maria G. R.; Martins, Alice M. C.Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:49:34Z No. of bitstreams: 1 S0001-37652009000200007.pdf: 536011 bytes, checksum: 846642c2588a4bd24f90ee703c7ddc86 (MD5); Made available in DSpace on 2013-08-22T18:49:35Z (GMT). No. of bitstreams: 1 S0001-37652009000200007.pdf: 536011 bytes, checksum: 846642c2588a4bd24f90ee703c7ddc86 (MD5) Previous issue date: 2009-06-01; Made availabl...