21 documents found, page 1 of 3
Sustained Increase of Paediatric Invasive Streptococcus Pyogenes Infections Dom...
Gouveia, C; Bajanca-Lavado, MP; Mamede, R; Araújo Carvalho, A; Rodrigues, F; Melo-Cristino, J; Ramirez, M; Friães, Aince autumn 2022, observed numbers of paediatric invasive group A Streptococcus infections in Portugal (n = 89) were higher than in pre-COVID-19 seasons. Between September 2022 and May 2023, the dominant diagnoses were pneumonia (25/79), mostly with empyema (20/25), and sepsis (22/79). A number of cases required admission to intensive care (27/79) and surgery (35/79), and the case fatality rate was 5.1% (4/79)....
A Phylogenomic Approach for the Analysis of Colistin Resistance-Associated Gene...
Elias, R; Spadar, A; Phelan, J; Melo-Cristino, J; Lito, L; Pinto, M; Gonçalves, L; Campino, S; Clark, TG; Duarte, A; Perdigão, JThe emergence of carbapenemase-producing Klebsiella pneumoniae strains has triggered the use of old antibiotics such as colistin. This is driving the emergence of colistin resistance in multidrug-resistant strains that underlie life-threatening infections. This study analyses the mutational diversity of 22 genes associated with colistin resistance in 140 K. pneumoniae clinical isolates integrated in a high-reso...
Imipenem-Relebactam Susceptibility in Enterobacterales Isolates Recovered from ...
Hernández-García, M; García-Castillo, M; Bou, G; Cercenado, E; Delgado-Valverde, M; Oliver, A; Pitart, C; Rodríguez-Lozano, J; Tormo, NImipenem-relebactam is a novel β-lactam-β-lactamase inhibitor combination. We evaluated the in vitro activity of imipenem-relebactam and comparators against Enterobacterales clinical isolates recovered in 8 Spanish and 11 Portuguese intensive care units (ICUs) (SUPERIOR, 2016-2017; STEP, 2017-2018). Overall, 747 Enterobacterales isolates (378 Escherichia coli, 252 Klebsiella spp., 64 Enterobacter spp., and 53 o...
Confronting Ceftolozane-Tazobactam Susceptibility in Multidrug-Resistant Entero...
Hernández-García, M; García-Fernández, S; García-Castillo, M; Melo-Cristino, J; Pinto, M; Gonçalves, E; Alves, V; Costa, E; Ramalheira, E; Sancho, LCeftolozane-tazobactam (C/T) is frequently used for infections caused by multidrug-resistant (MDR)-Enterobacterales isolates. Whole-genome sequencing (WGS, Illumina-Hiseq 4000/NovaSeq 6000, OGC, UK) was used to study the population structure, the resistome and the virulome of C/T-susceptible and -resistant MDR Escherichia spp. (n=30) and Klebsiella spp. (n=78) isolates, recovered from lower respiratory, intra-a...
Vitamin D-related polymorphisms and vitamin D levels as risk biomarkers of COVI...
Freitas, AT; Calhau, C; Antunes, G; Araújo, B; Bandeira, M; Barreira, S; Bazenga, F; Braz, S; Caldeira, D; Santos, SCR; Faria, A; Faria, D; Fraga, MVitamin D is a fundamental regulator of host defences by activating genes related to innate and adaptive immunity. Previous research shows a correlation between the levels of vitamin D in patients infected with SARS-CoV-2 and the degree of disease severity. This work investigates the impact of the genetic background related to vitamin D pathways on COVID-19 severity. For the first time, the Portuguese populatio...
In Vitro Activity of Ceftolozane-Tazobactam Against Enterobacterales and Pseudo...
García-Fernández, S; García-Castillo, M; Melo-Cristino, J; Pinto, M; Gonçalves, E; Alves, V; Vieira, AR; Ramalheira, E; Sancho, L, et al.The STEP surveillance study was designed to increase knowledge about distribution of multidrug-resistant (MDR) Enterobacterales and Pseudomonas aeruginosa in Portugal, focusing on the intensive care unit (ICU). Antimicrobial susceptibility of common agents was also evaluated and compared with that of one of the latest therapeutic introductions, ceftolozane-tazobactam (C/T). Clinical isolates of Enterobacterales...
In Vitro Activity of Ceftolozane-Tazobactam Against Enterobacterales and Pseudo...
García-Fernández, S; García-Castillo, M; Melo-Cristino, J; Pinto, M; Gonçalves, E; Alves, V; Vieira, AR; Ramalheira, E; Sancho, L; Diogo, J; Ferreira, RThe STEP surveillance study was designed to increase knowledge about distribution of multidrug-resistant (MDR) Enterobacterales and Pseudomonas aeruginosa in Portugal, focusing on the intensive care unit (ICU). Antimicrobial susceptibility of common agents was also evaluated and compared with that of one of the latest therapeutic introductions, ceftolozane-tazobactam (C/T). Clinical isolates of Enterobacterales...
Dominance of Vaccine Serotypes in Pediatric Invasive Pneumococcal Infections in...
Silva-Costa, C; Brito, MJ; Aguiar, SI; Lopes, JP; Ramirez, M; Melo-Cristino, JWe evaluated the impact of continued 13-valent pneumococcal conjugate vaccine (PCV13) use in the private market (uptake of 61%) in pediatric invasive pneumococcal disease (pIPD) in Portugal (2012-2015). The most frequently detected serotypes were: 3 (n = 32, 13.8%), 14 (n = 23, 9.9%), 1 (n = 23, 9.9%), 7F (n = 15, 6.4%), 19A (n = 13, 5.6%), 6B and 15B/C (both n = 12, 5.2%), and 24F, 10A and 12B (all with n = 10...
Streptococcus canis are a single population infecting multiple animal hosts des...
Pinho, MD; Foster, G; Pomba, C.; Machado, MP; Baily, JL; Kuiken, T; Melo-Cristino, J; Ramirez, M; Vaz, T; Giao, MABSTRACT - Streptococcus canis is an animal pathogen which occasionally causes infections in humans. The S. canis M-like protein (SCM) encoded by the scm gene, is its best characterized virulence factor but previous studies suggested it could be absent in a substantial fraction of isolates. We studied the distribution and variability of the scm gene in 188 S. canis isolates recovered from companion animals (n =...
Streptococcus pyogenes Causing Skin and Soft Tissue Infections Are Enriched in ...
Pato, C; Melo-Cristino, J; Ramirez, M; Friães, A; Portuguese Group for the Study of Streptococcal Infections.Although skin and soft tissue infections (SSTI) are the most common focal infections associated with invasive disease caused by Streptococcus pyogenes (Lancefield Group A streptococci - GAS), there is scarce information on the characteristics of isolates recovered from SSTI in temperate-climate regions. In this study, 320 GAS isolated from SSTI in Portugal were characterized by multiple typing methods and teste...