3 documents found, page 1 of 1
Application of nucleic acid mimics in fluorescence in situ hybridization
Oliveira, Ricardo; Azevedo, Andreia S.; Mendes, LuziaTraditionally, RNA and DNA probes are used in fluorescence in situ hybridization (FISH) methods for microbial detection and characterization of communities structure and diversity. However, the recent introduction of nucleic acid mimics (NAMs) has improved the robustness of the FISH methods in terms of sensitivity and specificity. Several NAMs have been used, of which the most relevant are peptide nucleic acid ...
#126. Nova estratégia para detetar e localizar patogénicos periodontais: a técn...
Mendes, Luzia; Rocha, Rui; Azevedo, Andreia S.; Henriques, Mariana; Pinto, Miguel G.; Azevedo, N. F.[Excerto] Objetivos: A compreensão da dinâmica periodontal biofilme-hospedeiro, in situ, é crucial para melhorar o diagnóstico e definir tratamentos mais racionais e eficazes. Este trabalho tem como objetivo o desenvolvimento de sondas de ácido peptídico nucleico (PNA), um mímico do DNA, para a identificac¸ão e localizac¸ão de Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans)ePorphyromonas gingivalis...
Novel strategy to detect and locate periodontal pathogens: The PNA-FISH technique
Mendes, Luzia; Rocha, Rui; Azevedo, Andreia S.; Ferreira, Catarina; Henriques, Mariana; Pinto, Miguel Gonçalves; Azevedo, Nuno FilipePurpose: We aim to develop peptic nucleic acid (PNA) probes for the identification and localization of Aggregatibacter actinomycetemcomintans and Porphyromonas gingivalis in sub-gingival plaque and gingival biopsies by Fluorescence in situ Hybridization (FISH). Methods A PNA probe was designed for each microorganism. The PNA-FISH method was optimized to allow simultaneous hybridization of both microorganisms wi...