6 documents found, page 1 of 1
Complete genome sequence of two deep-sea streptomyces isolates from madeira arc...
Albuquerque, P; Ribeiro, I; Correia, S; Mucha, AP; Tamagnini, P; Braga-Henriques, A; Carvalho, MdF; Mendes, MVThe deep-sea constitutes a true unexplored frontier and a potential source of innovative drug scaffolds. Here, we present the genome sequence of two novel marine actinobacterial strains, MA3_2.13 and S07_1.15, isolated from deep-sea samples (sediments and sponge) and collected at Madeira archipelago (NE Atlantic Ocean; Portugal). The de novo assembly of both genomes was achieved using a hybrid strategy that com...
The novel ECF56 SigG1-RsfG system modulates morphological differentiation and m...
Oliveira, R; Bush, MJ; Pires, SDS; Chandra, G; Casas-Pastor, D; Fritz, G; Mendes, MVExtracytoplasmic function (ECF) sigma factors are key transcriptional regulators that prokaryotes have evolved to respond to environmental challenges. Streptomyces tsukubaensis harbours 42 ECFs to reprogram stress-responsive gene expression. Among them, SigG1 features a minimal conserved ECF s2–s4 architecture and an additional C-terminal extension that encodes a SnoaL_2 domain, which is characteristic for ECF ...
The onset of tacrolimus biosynthesis in streptomyces tsukubaensis is dependent ...
Pires, SDS; Oliveira, R; Moradas-Ferreira, P; Mendes, MVThe oxidative stress response is a key mechanism that microorganisms have to adapt to changeling environmental conditions. Adaptation is achieved by a fine-tuned molecular response that extends its influence to primary and secondary metabolism. In the past, the role of the intracellular redox status in the biosynthesis of tacrolimus in Streptomyces tsukubaensis has been briefly acknowledged. Here, we investigat...
Chassis optimization as a cornerstone for the application of synthetic biology ...
Beites, T; Mendes, MVThe increased number of bacterial genome sequencing projects has generated over the last years a large reservoir of genomic information. In silico analysis of this genomic data has renewed the interest in bacterial bioprospecting for bioactive compounds by unveiling novel biosynthetic gene clusters of unknown or uncharacterized metabolites. However, only a small fraction of those metabolites is produced under l...
Streptomyces natalensis programmed cell death and morphological differentiation...
Beites, T; Oliveira, P; Rioseras, B; Pires, SD; Oliveira, R; Tamagnini, P; Moradas-Ferreira, P; Manteca, Á; Mendes, MVStreptomyces are aerobic Gram-positive bacteria characterized by a complex life cycle that includes hyphae differentiation and spore formation. Morphological differentiation is triggered by stressful conditions and takes place in a pro-oxidant environment, which sets the basis for an involvement of the oxidative stress response in this cellular process. Characterization of the phenotypic traits of Streptomycesn...
To Be or Not to Be a Pseudogene: A Molecular Epidemiological Approach to the mc...
Santos, CL; Nebenzahl-Guimaraes, H; Mendes, MV; Soolingen, D; Correia-Neves, MTuberculosis presents a myriad of symptoms, progression routes and propagation patterns not yet fully understood. Whereas for a long time research has focused solely on the patient immunity and overall susceptibility, it is nowadays widely accepted that the genetic diversity of its causative agent, Mycobacterium tuberculosis, plays a key role in this dynamic. This study focuses on a particular family of genes, ...