11 documents found, page 1 of 2
Enhancing biomarker detection in human serum for lung cancer diagnosis: aqueous...
Rosa, Marguerita E.; Mendes, Maria S. M.; Belchior, Diana C. V.; Coutinho, João A. P.; Silva, Francisca A. e; Freire, Mara G.Analysing biomarkers in human serum could be used as an effective and less invasive approach for the diagnosis of lung cancer; however, biomarker detection reliability is highly limited due to matrix effects. Herein, aqueous biphasic systems (ABS) are studied as platforms for human serum pretreatment, allowing the simultaneous depletion of high abundant proteins and biomarker extraction. By using ABS varying th...
Ionic liquid-based aqueous biphasic systems for improved detection of cancer bi...
Mendes, Maria S. M.; Chu, Virginia; Francisca A. e Silva; Freire, Mara G.; Conde, João P.Cancer poses a significant global health challenge with low survival rates often due to late-stage diagnoses.1 Early detection is crucial for effective treatment and monitoring. Human serum cancer biomarkers, like prostate specific antigen (PSA) and human epidermal growth factor receptor 2 (HER2), are key indicators of cancer development, treatment response, and disease recurrence. However, detecting these biom...
Aqueous two-phase systems as multipurpose tools to improve biomarker analysis
Mendes, Maria S. M.; Rosa, Marguerita E.; Ramalho, Fábio; Freire, Mara G.; e Silva, Francisca A.Biomarker analysis in biological samples can boost risk profiling, diagnosis, prognosis and clinical decision-making, overall improving patient care. However, due to the complexity of most biological samples, biomarker analysis is a time-, cost-, labor- and resource-intensive task, commonly struggling with the lack of accurate results. To improve biomarker analysis, it is critical to develop efficient strategie...
Improved accuracy in pentraxin-3 quantification assisted by aqueous biphasic sy...
Mendes, Maria S. M.; Rosa, Marguerita E.; Coutinho, João A. P.; Freire, Mara G.; E Silva, Francisca A.Although pentraxin-3 holds promise as a diagnosis/prognosis biomarker of microbial infections and lung cancer, its analysis in human serum can be constrained by matrix effects caused by high abundance proteins - human serum albumin and immunoglobulin G. Aqueous biphasic systems composed of polymers and citrate buffer are here proposed as a serum pretreatment step to improve the accuracy of pentraxin-3 analysis....
Tailored pretreatment of serum samples and biomarker extraction afforded by ion...
Rosa, Marguerita E.; Mendes, Maria S. M.; Carmo, Eduarda; Conde, João P.; Coutinho, João A. P.; Freire, Mara G.; Silva, Francisca A.Prostate cancer serum biomarkers, such as the U.S. Food and Drug Administration (FDA)-approved prostate specific antigen (PSA), are valuable markers of diagnosis, response to treatment and disease progression. Despite the expected benefits of using PSA to better manage the mortality and morbidity associated to prostate cancer, sample complexity and interference within analysis make its quantification in serum a...
Extraction of breast cancer biomarkers from human serum using ionic liquid-base...
Mendes, Maria S. M.; Souza, Maria C.; Freire, Mara G.; Silva, Francisca A. eNo abstract available.
Improved sample pretreatment and biomarker extraction using aqueous biphasic sy...
Silva, Francisca A. e; Rosa, Marguerita E.; Mendes, Maria S. M.; Carmo, Eduarda; Coutinho, João A. P.; Freire, Mara G.No abstract available.
Deep Eutectic Solvent Formulations and Alginate-Based Hydrogels as a New Partne...
Pedro, Sónia N.; Mendes, Maria S. M.; Neves, Bruno M.; Almeida, Isabel Filipa; Costa, Paulo; Correia-Sá, Inês; Vilela, Carla; Freire, Mara G.The transdermal administration of nonsteroidal anti-inflammatory drugs (NSAIDs) is a valuable and safer alternative to their oral intake. However, most of these drugs display low water solubility, which makes their incorporation into hydrophilic biopolymeric drug-delivery systems difficult. To overcome this drawback, aqueous solutions of bio-based deep eutectic solvents (DES) were investigated to enhance the so...
Enhancing human serum pretreatment using ionic liquids as adjuvants in polymer-...
Rosa, Marguerita E.; Mendes, Maria S. M.; Coutinho, João A. P.; Silva, Francisca A. e; Freire, Mara G.The quantification of cancer biomarkers in human serum is a viable approach to expedite diagnosis [1]. Nevertheless, the accuracy and precision of biomarkers’ quantification is currently limited by their low concentrations and tendency to be masked by high-abundance proteins such as immunoglobulin G (IgG) and human serum albumin (HSA) [2]. To improve biomarkers’ detection and quantification, a simple serum pret...
Pretreatment of biological samples using three-phase partitioning systems: a to...
Mendes, Maria S. M.; Rosa, Marguerita E.; Silva, Francisca A. e; Freire, Mara G.Early diagnosis of pathologies represents the ideal approach to boost the survival chances of patients, while contributing to reduce the burden of the disease. In this framework, biomarkers, such as proteins, hold potential. Nevertheless, biomarker detection remains a technically difficult task as biological samples, such as human serum, are complex and biomarkers are low abundant molecules. As a result, a lack...