45 documents found, page 1 of 5
Class II two-peptide lanthipeptide proteases: exploring LicTP for biotechnologi...
Barbosa, Joana C.; Mösker, Eva; Faria, Raquel; Süssmuth, Roderich D.; Mendo, Sónia; Caetano, TâniaThe enzymatic machinery involved in the biosynthesis of lantibiotic is an untapped source of proteases with different specificities. Lanthipeptide biosynthesis requires proteolysis of specific target sequences by known proteases, which are encoded by contiguous genes. Herein, the activity of lichenicidin A2 (LicA2) trimming proteases (LicP and LicT) was investigated in vivo. Firstly, the impact of some residues...
Oh, deer! How worried should we be about the diversity and abundance of the fae...
Dias, Diana; Fonseca, Carlos; Caetano, Tânia; Mendo, SóniaThe emergence of antimicrobial resistance (AMR) is a global threat to public health. Antimicrobials are used in animal production and human medicine, which contribute to the circulation of antibiotic resistance genes (ARGs) in the environment. Wildlife can be reservoirs of pathogens and resistant bacteria. Furthermore, anthropogenic pressure can influence their resistome. This work aimed to study the AMR of the...
Insights into the mode of action of the two-peptide lantibiotic lichenicidin
Barbosa, Joana C.; Gonçalves, Sónia; Makowski, Marcin; Silva, Ítala Cristine; Caetano, Tânia; Schneider, Tanja; Mösker, Eva; Süssmuth, Roderich D.Lantibiotics are promising candidates to address the worldwide problem of antibiotic resistance. They belong to a class of natural compounds exhibiting strong activity against clinically relevant Gram-positive bacterial strains, including methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE). Lichenicidin is a class II two-peptide lantibiotic. The presence of the two matu...
Peptone from casein, an antagonist of nonribosomal peptide synthesis: a case st...
Covas, Cláudia; Almeida, Beatriz; Esteves, Ana Cristina; Lourenço, Joana; Domingues, Pedro; Caetano, Tânia; Mendo, SóniaNovel natural products are urgently needed to address the worldwide incidence of bacterial resistance to antibiotics. Extreme environments are a major source of novel compounds with unusual chemical structures. Pedobacter lusitanus NL19 is a new bacterial species that was isolated from one such environment and which produces compounds with potent activity against relevant microorganisms in the clinical, food, v...
Carbapenem resistant Enterobacteriaceae from port areas in São Paulo State (Bra...
Andrade, Vanessa Costa [UNESP]; Caetano, Tânia; Mendo, Sónia; Oliveira, Ana Júlia Fernandes Cardoso de [UNESP]Made available in DSpace on 2020-12-12T02:18:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Office of Electricity Delivery and Energy Reliability; Centro de Estudos Ambientais e Marinhos, Universidade de Aveiro; Coastal areas with important economic activities have high levels of contamination by metals, pathogenic bacteria, ...
Evolving trends in next-generation probiotics: a 5W1H perspective
Almeida, Diana; Machado, Daniela; Andrade, José Carlos; Mendo, Sónia; Gomes, Ana Maria; Freitas, Ana CristinaIn recent years, scientific community has been gathering increasingly more insight on the dynamics that are at play in metabolic and inflammatory disorders. These rapidly growing conditions are reaching epidemic proportions, bringing clinicians and researcher’s new challenges. The specific roles and modulating properties that beneficial/probiotic bacteria hold in the context of the gut ecosystem seem to be key ...
Chemical availability versus bioavailability of potentially toxic elements in m...
Rodríguez-Seijo, Andrés; Lourenço, Joana; Arenas-Lago, Daniel; Mendo, Sónia; Vega, Flora A.; Pereira, RuthAbandoned mining and quarry areas are sources of potentially toxic elements (PTEs), through lixiviates or transfer processes of bioavailable fractions from mining wastes and tailings. In this study, earthworms (Eisenia fetida Savigny, 1826) were exposed for 28 days to two mining soils from a lead/zinc mine and two quarry soils from an old serpentine quarry. Despite their pseudo total metal contents, a previous ...
Wild boar as a reservoir of antimicrobial resistance
Torres, Rita Tinoco; Fernandes, Joana; Carvalho, João; Cunha, Mónica V.; Caetano, Tânia; Mendo, Sónia; Serrano, Emmanuel; Fonseca, CarlosAntimicrobial resistance (AMR) has been recognized as an emerging and growing problem worldwide. Knowledge concerning AMR bacteria circulating in wildlife is currently limited, although it could provide important insights into AMR emergence and persistence. Across Europe, wild boar (Sus scrofa) populations have dramatically increased their distribution and number over the last decades. In the context of AMR dyn...
Wild boar as a reservoir of antimicrobial resistance
Torres, Rita Tinoco; Fernandes, Joana; Carvalho, João; Cunha, Mónica V.; Caetano, Tânia; Mendo, Sónia; Serrano, Emmanuel; Fonseca, CarlosAntimicrobial resistance (AMR) has been recognized as an emerging and growing problem worldwide. Knowledge concerning AMR bacteria circulating in wildlife is currently limited, although it could provide important insights into AMR emergence and persistence. Across Europe, wild boar (Sus scrofa) populations have dramatically increased their distribution and number over the last decades. In the context of AMR dyn...
Lipidomic signature of Bacillus licheniformis I89 during the different growth p...
Lopes, Celestina; Barbosa, Joana; Maciel, Elisabete; da Costa, Elisabete; Alves, Eliana; Domingues, Pedro; Mendo, Sónia; Domingues, M. Rosário M.Bacillus licheniformis I89 is a non-pathogenic, Gram-positive bacterium, frequently found in soil. It has several biotechnological applications as producer of valuable compounds such as proteases, amylases, surfactants, and lantibiotics. Herein, it is reported the identification of the polar lipidome of B. licheniformis I89 during the different growth phases (lag, exponential and stationary) at 37 °C. The analy...