6 documents found, page 1 of 1
Use of Tunable Copolymers in Aqueous Biphasic Systems for Extractive Bioconvers...
Mendonça, Carlos M. N.; Veríssimo, Nathalia V.; Pereira, Wellison A.; Cunha, Paula M.; Vitolo, Michele; Converti, Attilio; Kurnia, Kiki AdiAqueous biphasic systems (ABSs) based on sodium polyacrylate (NaPA), ethylene oxide/propylene oxide (EO/PO) polymers, and (EO)x-(PO)y-(EO)x triblock copolymers were prepared and applied aiming at continuous fructooligosaccharide (FOS) production and separation. EO/PO hydrophilicity/hydrophobicity balance had a significant effect on ABS formation. To develop an integrated process including the continuous enzymat...
Characterization of levan produced by a Paenibacillus sp. isolated from Brazili...
Mendonça, Carlos M. N.; Oliveira, Rodrigo C.; Freire, Rominne K. B.; Piazentin, Anna C. M.; Pereira, Wellison A.; Gudiña, Eduardo JoséA levan-type fructooligosaccharide was produced by a Paenibacillus strain isolated from Brazilian crude oil, the purity of which was 98.5% after precipitation with ethanol and dialysis. Characterization by FTIR, NMR spectroscopy, GC-FID and ESI-MS revealed that it is a mixture of linear (26) fructosyl polymers with average degree of polymerization (DP) of 18 and branching ratio of 20. Morphological structure an...
Characterization of levan produced by a Paenibacillus sp. isolated from Brazili...
Mendonça, Carlos M. N.; Oliveira, Rodrigo C.; Freire, Rominne K. B.; Piazentin, Anna C. M.; Pereira, Wellison A.; Gudiña, Eduardo J.A levan-type fructooligosaccharide was produced by a Paenibacillus strain isolated from Brazilian crude oil, the purity of which was 98.5% after precipitation with ethanol and dialysis. Characterization by FTIR, NMR spectroscopy, GC-FID and ESI-MS revealed that it is a mixture of linear β(2 → 6) fructosyl polymers with average degree of polymerization (DP) of 18 and branching ratio of 20. Morphological structur...
Understanding the interactions of imidazolium-based ionic liquids with cell mem...
Mendonça, Carlos M. N.; Balogh, Debora T.; Barbosa, Simone C.; Sintra, Tânia E.; Ventura, Sónia P. M.; Martins, Luís F. G.; Morgado, PedroCell membrane models have been used to evaluate the interactions of various imidazolium-based ionic liquids (ILs) with Langmuir monolayers of two types of phospholipids and cholesterol. Data from surface pressure isotherms, Brewster angle microscopy (BAM) and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) pointed to significant effects on the monolayers of 1,2-dipalmitoylsn-glycer...
An integrated process combining the reaction and purification of PEGylated prot...
Santos, João H. P. M.; Mendonça, Carlos M. N.; Silva, Amanda R. P.; Oliveira, Ricardo P. S.; Pessoa, Adalberto; Coutinho, João A. P.A downstream process combining PEGylation reaction and the use of enzyme conjugates acting as phase-forming components of aqueous biphasic systems (ABS) is proposed here. In this approach, citrate buffer (pH = 7.0) was used simultaneously to stop the reaction (avoiding the use of hydroxylamine) and as a phase forming agent inducing the phase separation of the PEGylated proteins. The partition of the bioconjugat...
Understanding the interactions of imidazolium-based ionic liquids with cell mem...
Mendonça, Carlos M. N.; Balogh, Debora T.; Barbosa, Simone C.; Sintra, Tânia E.; Ventura, Sónia P. M.; Martins, Luís F. G.; Morgado, PedroCell membrane models have been used to evaluate the interactions of various imidazolium-based ionic liquids (ILs) with Langmuir monolayers of two types of phospholipids and cholesterol. Data from surface pressure isotherms, Brewster angle microscopy (BAM) and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) pointed to significant effects on the monolayers of 1,2-dipalmitoyl-sn-glyce...