2 documents found, page 1 of 1
Polyclonal anti-intimin antibody: immunological characterization and its use in...
Koga,Paula Célia Mariko; Menezes,Caroline Anunciação; Lima,Flávia Afonso; Nara,Júlia Mitico; Magalhães,Caroline Arantes; Cianciarullo,Aurora MarquesIntimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detec...
Capture immunoassay for LT detection produced by enterotoxigenic Escherichia co...
Menezes,Caroline Anunciação; Gonçalves,Danielle Silva; Amianti,Jackeline; Fernandes,Irene; Taddei,Carla Romano; Koga,Paula Célia MarikoA capture enzyme-linked immunosorbent assay (ELISA), which detects LT-I toxin produced by enterotoxigenic Escherichia coli strains, has beendeveloped. This capture assay was performed using the IgG enriched fraction of anti-LT-I antiserum and IgG2b anti-LT-I monoclonal antibody and allowed a clear distinction between E. coli LT-I - producing and non-producing strains. The estimated accuracy of the assay is 78% ...