9 documents found, page 1 of 1
A Gß protein and the TupA Co-Regulator bind to protein kinase A Tpk2 to act as ...
Janganan, Thamarai K.; Gongyou Chen; Daliang Chen; Menino, João F.; Rodrigues, Fernando José dos Santos; Walmsley, Maria I. Borges; Walmsley, Adrian R.The human pathogenic fungus Paracoccidioides brasiliensis (Pb) undergoes a morphological transition from a saprobic mycelium to pathogenic yeast that is controlled by the cAMP-signaling pathway. There is a change in the expression of the Gß-protein PbGpb1, which interacts with adenylate cyclase, during this morphological transition. We exploited the fact that the cAMP-signaling pathway of Saccharomyces cerevisi...
Clinical Epidemiology of Buruli ulcer from Benin (2005-2013): effect of time-de...
Capela, Carlos; Sopoh, Ghislain E.; Houezo, Jean G.; Fiodessihoué, René; Dossou, Ange D.; Costa, Patrício Soares; Fraga, Alexandra G.; Menino, João F.Buruli Ulcer (BU) is a neglected infectious disease caused by Mycobacterium ulcerans that is responsible for severe necrotizing cutaneous lesions that may be associated with bone involvement. Clinical presentations of BU lesions are classically classified as papules, nodules, plaques and edematous infiltration, ulcer or osteomyelitis. Within these different clinical forms, lesions can be further classified as s...
Functional characterization of a xylose transporter in Aspergillus nidulans
Rodrigues, Fernando José dos Santos; Menino, João F.; Colabardini, Ana Cristina; Ries, Laure Nicolas Annick; Brown, Neil AndrewBACKGROUND: The production of bioethanol from lignocellulosic feedstocks will only become economically feasible when the majority of cellulosic and hemicellulosic biopolymers can be efficiently converted into bioethanol. The main component of cellulose is glucose, whereas hemicelluloses mainly consist of pentose sugars such as D-xylose and L-arabinose. The genomes of filamentous fungi such as A. nidulans encode...
P. brasiliensis virulence is affected by SconC, the negative regulator of inorg...
Menino, João F.; Saraiva, Margarida; Gomes, Jéssica Angélica Rezende; Sturme, Mark H. J.; Pedrosa, Jorge; Castro, António G.; Ludovico, PaulaConidia/mycelium-to-yeast transition of Paracoccidioidesbrasiliensis is a critical step for the establishment of paracoccidioidomycosis, a systemic mycosis endemic in Latin America. Thus, knowledge of the factors that mediate this transition is of major importance for the design of intervention strategies. So far, the only known pre-requisites for the accomplishment of the morphological transition are the tempe...
Paracoccidioides brasiliensis: virulence factors and host susceptibility
Menino, João F.In the last decades, significant advances were accomplished on the study of neglected fungal diseases, mainly in defining the pathogen genetics and host risk factors. Paracoccidioidomycosis, a mycosis caused by Paracoccidioides species, is one of these diseases, and it is estimated to affect 10 million individuals in countries from Latin America. Over the last years, genomic and transcriptome studies on this fu...
Identification of glucose transporters in Aspergillus nidulans
Reis, Thaila Fernanda dos; Menino, João F.; Pedro Bom, Vinícius Leite; Brown, Neil Andrew; Colabardini, Ana Cristina; Savoldi, Marcelao characterize the mechanisms involved in glucose transport, in the filamentous fungus Aspergillus nidulans, we have identified four glucose transporter encoding genes hxtB-E. We evaluated the ability of hxtB-E to functionally complement the Saccharomyces cerevisiae EBY.VW4000 strain that is unable to grow on glucose, fructose, mannose or galactose as single carbon source. In S. cerevisiae HxtB-E were targeted ...
Morphological heterogeneity of Paracoccidioides brasiliensis: relevance of the ...
Menino, João F.; Osório, Nuno S.; Sturme, Mark H. J.; Barros, Diana Cláudia Martins Costa; Alves, Ana G. Gomes; Almeida, A. J.; Costa, Patrício SoaresParacoccidioides brasiliensis budding pattern and polymorphic growth were previously shown to be closely linked to the expression of PbCDC42 and to infl uence the pathogenesis of the fungus. In this work we conducted a detailed morphogenetic evaluation of the yeast-forms of 11 different clinical and environmental P. brasiliensis isolates comprising four phylogenetic lineages (S1, PS2, PS3 and Pb01-like), as wel...
Functionality of the paracoccidioides mating α-pheromone-receptor system
Gomes, Jéssica Angélica Rezende; Alves, Ana G. Gomes; Menino, João F.; Coelho, Marco; Ludovico, Paula; Gonçalves, Paula; Sturme, Mark H. J.Recent evidence suggests that Paracoccidioides species have the potential to undergo sexual reproduction, although no sexual cycle has been identified either in nature or under laboratory conditions. In the present work we detected low expression levels of the heterothallic MAT loci genes MAT1-1 and MAT1-2, the a-pheromone (PBa) gene, and the a- and apheromone receptor (PREB and PREA) genes in yeast and mycelia...
Gene knockdown in Paracoccidioides brasiliensis using antisense RNA
Menino, João F.; Almeida, A. J.; Rodrigues, Fernando José dos SantosParacoccidioides brasiliensis is a thermal dimorphic fungus which at host environment exhibits a multinucleated and multibudding yeast form. The cellular and molecular mechanisms underlying these phenotypes remain to be clarified, mostly due to the absence of efficient classical genetic and molecular techniques. Here we describe a method for gene expression knockdown in P. brasiliensis by antisense RNA (aRNA) t...