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Detoxification of atrazine-contaminated wastewater by granular sludge

Costa, Mariana Filipa Guedes; Mesquita, D. P.; Salvador, Andreia Filipa Ferreira; Silva, Ana Rita Pereira; Ferreira, Eugénio C.

Atrazine (ATZ) is a widely used herbicide associated with ecological and human health risks, making its removal from wastewater a priority. This study evaluates, for the first time, the performance of an aerobic granular sludge sequencing batch reactor (AGS-SBR) for the detoxification of ATZ-contaminated wastewater. The system consistently achieved high removal efficiencies (?95%) of organic matter and phosphat...


Comparative assessment of two bioproducts using quantitative image analysis

Ucha, C.; Val del Río, Angeles; Pedrouso, A.; Mosquera-Corral, A.; Mesquita, D. P.

Polyhydroxyalkanoates (PHA) and triacylglycerides (TAG) could be used as bioplastic and precursors of biodiesel, respectively. To ensure a more controlled and efficient operation of the reactor, rapid monitoring of bioproducts accumulation is essential for enabling precise process adjustments and efficiency. Usually, gas chromatography (GC) or high-performance liquid chromatography (HPLC) are the methods used f...


Monitoring the stability of aerobic granular sludge under increasing fractions ...

Silva, Sérgio Alves; Duarte, Maria Salomé Lira; Amaral, A. Luís; Ferreira, Eugénio C.; Alves, M. M.; Mesquita, D. P.

This work investigates the effects of increasing fraction of slowly biodegradable chemical oxygen demand (sbCOD) on the morphology, stability, and performance of aerobic granular sludge (AGS) used for wastewater treatment. A sequencing batch reactor (SBR) was supplied with synthetic wastewater containing acetate as readily biodegradable COD (rbCOD) and increasing concentrations of oleate as slowly biodegradable...


Impact assessment of carbamazepine on aerobic granular sludge: monitoring morph...

Costa, Mariana; Mesquita, D. P.; Salvador, Andreia Filipa Ferreira; Silva, Ana Rita Pereira; Ferreira, Eugénio C.; Quintelas, Cristina Maria Catalão

Aerobic granular sludge (AGS) technology for treating pharmaceutical-contaminated wastewater has sparked a great deal of interest in recent decades. However, the prolonged effect of these pollutants in AGS systems still requires further studies. In this study, an aerobic granular sludge sequencing batch reactor (AGS-SBR) was used to treat synthetic wastewater containing carbamazepine (CBZ) at varying concentrat...


Pharmaceutical removal through granular sludge systems: performance, operationa...

Costa, M.; Mesquita, D. P.; Ferreira, Eugénio C.; Quintelas, Cristina Maria Catalão

Pharmaceuticals may end up in the environment through untreated wastewater, improper disposal of unused and expired pharmaceutical formulations, or effluents from manufacturing facilities. Many reports focusing on the occurrence of pharmaceuticals in the environment have been published in recent years. This environmental problem is also an issue of special concern for the European Union, which recommends the mo...


Assessment of extracellular polymeric substances in activated sludge using Indi...

Mesquita, D. P.; Melo, António; Costa, Joana Sofia Gomes; Ferreira, Eugénio C.

Background: Extracellular polymeric substances (EPS), including polysaccharides (PS), proteins (PN), and humic acid substances (HAS), that compose loosely bound EPS (LB-EPS) and tightly bound EPS (TB-EPS), are crucial for the structural and functional properties of activated sludge (AS). Traditional methods for EPS quantification are time-consuming and labour-intensive. This study explores an innovative approac...


Bioproducts assessment using quantitative image analysis in a mixed microbial c...

Ucha, C.; Val del Río, A.; Pedrouso, A.; Mosquera-Corral, A.; Mesquita, D. P.

The use of image analysis for the quantification of polyhydroxyalkanoates (PHAs) and triacylglycerides (TAGs) produced by a mixed microbial culture fed with used cooking oil was studied. Three different stains were used, the Sudan Black B (SBB), the Nile Blue A (NBA) and the BODIPY 493/503. Acquired images were analysed using Matlab R2024b software, and bioproduct (PHA and TAG) accumulation was predicted throug...


Unravelling microbiome changes in aerobic granular sludge saline wastewater tre...

Paulo, Ana M. S.; Salazar, Oihane; Costa, Joana Sofia Gomes; Mesquita, D. P.; Ferreira, Eugénio C.; Castro, Paula M. L.; Amorim, Catarina L.

Saline wastewaters mainly result from various industrial activities. In response to water shortage, seawater is increasingly utilized for diverse purposes, leading to an increased production of saline wastewater. The presence of salts in wastewater frequently impairs the efficiency of biological wastewater treatment technologies. Among these, aerobic granular sludge (AGS) has emerged as the most effective aerob...


Tratamento de águas residuais sintéticas contaminadas com carbamazepina usando ...

Costa, Mariana; Mesquita, D. P.; Salvador, Andreia Filipa Ferreira; Silva, Ana R.; Ferreira, Eugénio C.; Quintelas, Cristina Maria Catalão

Um reator descontínuo sequencial (SBR) inoculado com lamas granulares (AGS) foi utlizado para tratar água residual sintética contaminada com carbamazepina (CBZ) a três concentrações distintas: 2, 5 e 8 mg/L. As maiores taxas de remoção foram observadas a 8 mg/L, tendo-se, no entanto, observado grandes oscilações na eficiência de remoção ao longo dos três ensaios. Estas oscilações devem-se, muito provavelmente, ...


Innovative technologies for emerging issues in pharmaceuticals

Quintelas, Cristina; Mesquita, D. P.; Ferreira, Eugénio C.

Pharmaceutical contaminants have been detected in the environment all over the world. The main sources responsible for their appearance in aquatic, soils and air environments include human usage for personal health or cosmetic reasons, veterinary usage to enhance growth or health of livestock and agricultural usage to fertilize soil and fight pests. The adverse effects of pharmaceuticals include toxicity, devel...


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