86 documents found, page 1 of 9
Assessing process variable effects on laccase-mediated treatment for enhanced s...
Michelin, Michele; Gomes, Daniel Gonçalves; Freitas, Emanuelle N.; Alnoch, Robson C.; Salgado, José Carlos S.; Coelho, Eduardo João LouroLignocellulosic materials (LCM) have gained popularity as a substitute for petroleum, enabling the production of biofuels and various compounds. Eucalyptus bark residue (EBR), generated by pulp and paper mills, is a prime illustration of such materials. The conversion of holocellulose in these materials typically involves the application of enzymes. Nevertheless, these materials may still contain lignin, widely...
Immobilization of laccase on grape seed-derived hydrochar and biochar as sustai...
Sá, Helena Isabel Oliveira; Michelin, Michele; Tavares, T.; Sanroman, Maria Angeles; Rosales, Emilio; Neves, Isabel C.Pharmaceutical active compounds pose a serious global threat due to their continuous release into aquatic systems. This study explores an innovative approach for the degradation of acetaminophen (APAP), sulfamethoxazole (SMX) and tetracycline (TC) by the covalent immobilization of crude laccase from Lentinus sajor-caju on grape seed-derived biochar (GS-BC-Lac) and hydrochar (GS-HC-Lac). Characterization through...
Customized decorticator machine for improved Azorean PALF extraction
Chaves, Diego Morais; Araújo, Joana Carolina Carvalho; Marinho, Elina Margarida Ribeiro; Michelin, Michele; Fangueiro, Raúl; Ferreira, Diana P.This work features for the first time the optimization of Azorean PALF (pineapple leaf fiber) extraction using an innovative adjustable decorticator machine. Additionally, it presents a comparative study on the quality of the extracted fibers compared to those obtained using the conventional hand-scraping method. The main parameters of the customized extraction machine—rotor speed, the horizontal and vertical p...
Entrapment of laccase-halloysite nanotubes in chitosan beads for pharmaceutical...
Sá, Helena Isabel Oliveira; Silva, Bruna Andreia Nogueira Airosa; Tavares, T.; Michelin, MicheleThe presence of pharmaceuticals in water has emerged as a significant environmental concern. This study investigated the degradation of sulfamethoxazole (SMX), tetracycline (TC) and acetaminophen (APAP) using laccase from Lentinus sajor-caju immobilized by adsorption on halloysite nanotubes (HNT) and subsequent entrapment in chitosan (CS) beads. The immobilization process was successfully confirmed through Tran...
Pleurotus ostreatus and lentinus sajor-caju laccases for sulfamethoxazole biotr...
Sá, Helena; Michelin, Michele; Silvério, Sara Isabel Cruz; Polizeli, Maria de Lourdes T. M.; Silva, Ana Rita Pereira; Pereira, Luciana; Tavares, T.Sulfamethoxazole (SMX) is an antibiotic commonly used to treat human and animal infections. Therefore, it is one of the most identified pharmaceuticals worldwide in surface water and wastewater treatment plants. This work exploited Pleurotus ostreatus and Lentinus sajor-caju to produce laccase by using agricultural and food residues as substrates, specifically vine pruning and brewer's spent grains. The fungal ...
First insight into lignin valorization as a promising biopolymer for the modula...
Pereira, Ana Rita; Brandão, Elsa; Pinto, Joana; Pinho, Paula Guedes de; Costa, Carina A. E.; Rodrigues, Alírio E.; Michelin, Michele; Soares, SusanaLignosulfonate (LS), kraft lignin (KL), and organosolv lignin (OL) were evaluated as potential modulating agents of the physicochemical properties of Port wine at two different concentrations for 7 and 30 days. KL and LS demonstrated the ability to remove proteins and potentiate the anthocyanin concentration. LS reduced the tannin content and the interaction of salivary acidic proline-rich proteins with wine ph...
Strategies for the incorporation of organosolv lignin in hydroxypropyl methylce...
Silva, Lúcio; Colussi, F.; Martins, Joana T.; Vieira, Jorge Miguel Magalhães; Pastrana, Lorenzo M.; Teixeira, J. A.; Cerqueira, Miguel A.Organosolv lignin extracted from vine pruning residues was added to hydroxypropyl methylcellulose (HPMC)-based films using three strategies: i) lignin incorporated into the film (lignin-based film), ii) lignin nanoparticles (LNPs) incorporated into the film (LNPs-based film), and iii) lignin coated on HPMC films' surface (lignin-coated film). The films obtained were evaluated in terms of morphology, water barri...
Challenges in using ionic liquids for cellulosic ethanol production
Colussi, F.; Rodríguez, Héctor; Michelin, Michele; Teixeira, J. A.The growing need to expand the use of renewable energy sources in a sustainable manner, providing greater energy supply security and reducing the environmental impacts associated with fossil fuels, finds in the agricultural by-product bioethanol an economically viable alternative with significant expansion potential. In this regard, a dramatic boost in the efficiency of processes already in place is required, r...
Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pre...
Michelin, Michele; Ximenes, Eduardo; Polizeli, Maria de Lourdes T. M.; Ladisch, Michael R.Relatively few studies have addressed the characterization of sugarcane straw (SCS) for production fermentable sugars through enzyme hydrolysis. Straw is a major co-product of the sugarcane harvest in Brazil that has potential to sustainably increase cellulosic feedstocks in Brazil by 50%. Pretreatment of 10% w/v straw with liquid hot water (LHW) at 180°C for 50min (severity, So, of 4.05), solubilizes hemicellu...
Edible alginate-based films with anti-SARS-CoV-2 activity
Cerqueira, Miguel A.; Leite, Ana C. C. O.; Tomás, Ana L.; Reichel, Anna; Silva, Patrícia M.; Santos, Nuno C.; Michelin, Michele; Fuciños, PabloThe viability of SARS-CoV-2 on food surfaces and its propagation through the food chain has been discussed by several stakeholders, as it may represent a serious public health problem, bringing new challenges to the food system. This work shows for the first time that edible films can be used against SARS-CoV-2. Sodium alginate-based films containing gallic acid, geraniol, and green tea extract were evaluated i...