12 documents found, page 1 of 2
An improved reference genome and first organelle genomes of Quercus suber
Usié, Ana; Serra, Octávio; Barros, Pedro M.; Barbosa, Pedro; Leão, Célia; Capote, Tiago; Almeida, Tânia; Rodrigues, Leandra; Carrasquinho, IsabelCork oak (Quercus suber L.) is an ecologically and economically important evergreen tree species native to the Mediterranean region and widespread in southwest Europe and northwest Africa. An improved genome assembly of cork oak using a combination of Illumina and PacBio sequencing is presented in this study. The assembled genome contains 2351 scaffolds longer than 1000 bp, accounting for 765.7 Mbp of genome si...
Molecular Defense Response of Pine Trees (Pinus spp.) to the Parasitic Nematode...
Modesto, Inês; Mendes, André; Carrasquinho, Isabel; Miguel, Célia M.Pine wilt disease (PWD) is a severe environmental problem in Eastern Asia andWestern Europe, devastating large forest areas and causing significant economic losses. This disease is caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, a parasitic migratory nematode that infects the stem of conifer trees. Here we review what is currently known about the molecular defense response in pine trees afte...
SNP Detection in Pinus pinaster Transcriptome and Association with Resistance t...
Modesto, Inês; Inácio, Vera; Novikova, Polina; Carrasquinho, Isabel; Van de Peer, Yves; Miguel, Célia M.Pinewood nematode (PWN, Bursaphelenchus xylophilus) is the causal agent of pine wilt disease (PWD), which severely affects Pinus pinaster stands in southwestern Europe. Despite the high susceptibility of P. pinaster, individuals of selected half-sib families have shown genetic variability in survival after PWN inoculation, indicating that breeding for resistance can be a valuable strategy to control PWD. In thi...
Insights into the mechanisms implicated in Pinus pinaster resistance to pinewoo...
Modesto, Inês; Sterck, Lieven; Arbona, Vicent; Gómez-Cadenas, Aurelio; Carrasquinho, Isabel; Van de Peer, Yves; Miguel, Célia M.Pine wilt disease (PWD), caused by the plant–parasitic nematode Bursaphelenchus xylophilus, has become a severe environmental problem in the Iberian Peninsula with devastating effects in Pinus pinaster forests. Despite the high levels of this species’ susceptibility, previous studies reported heritable resistance in P. pinaster trees. Understanding the basis of this resistance can be of extreme relevance for fu...
The draft genome sequence of cork oak
Ramos, António Marcos; Usié, Ana; Barbosa, Pedro; Barros, Pedro M.; Capote, Tiago; Chaves, Inês; Simões, Fernanda; Abreu, Isabl; Carrasquinho, IsabelCork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork oak genome. We followed a de novo assembly strategy based on hi...
The draft genome sequence of cork oak
Ramos, António Marcos; Usié, Ana; Barbosa, Pedro; Barros, Pedro M.; Capote, Tiago; Chaves, Inês; Simões, Fernanda; Abreu, Isabl; Carrasquinho, IsabelCork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork oak genome. We followed a de novo assembly strategy based on hi...
Characterization of the cork oak transcriptome dynamics during acorn development
Miguel, Andreia; de Vega-Bartol, José; Marum, Liliana; Chaves, Inês; Santo, Tatiana; Leitão, J. M.; Varela, Maria C.; Miguel, Célia M.Background: Cork oak (Quercus suber L.) has a natural distribution across western Mediterranean regions and is a keystone forest tree species in these ecosystems. The fruiting phase is especially critical for its regeneration but the molecular mechanisms underlying the biochemical and physiological changes during cork oak acorn development are poorly understood. In this study, the transcriptome of the cork oak ...
A comprehensive assessment of the transcriptome of cork oak (Quercus suber) thr...
Pereira-Leal, José B.; Abreu, Isabel A.; Alabaça, Cláudia S.; Almeida, Maria H.; Almeida, Paulo; Almeida, Tânia; Amorim, Maria I.; Araújo, SusanaBackground: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic inform...
Analysis of genetic stability at SSR loci during somatic embryogenesis in marit...
Marum, Liliana; Rocheta, Margarida; Maroco, João; Oliveira, Maria Margarida; Miguel, Célia M.Somatic embryogenesis (SE) is a propagation tool of particular interest for accelerating the deployment of new high-performance planting stock in multivarietal forestry. However, genetic conformity in in vitro propagated plants should be assessed as early as possible, especially in long-living trees such as conifers. The main objective of this work was to study such conformity based on genetic stability at simp...
Improved in vitro rooting of Prunus dulcis Mill. cultivars
Tereso, Susana; Miguel, Célia M.; Mascarenhas, M.; Roque, A.; Trindade, H.; Maroco, João; Oliveira, Maria MargaridaA highly reproducible system was developed for efficient rooting of cultivars Boa Casta (BC) and Peneda and a BC seedling-derived clone (BC VII) of almond (Prunus dulcis Mill.). Twenty-four accessions derived from the clone BC VII and subjected to various in vitro culture treatments were screened. The long induction pre-treatment (LIP, 5 d), the brief induction pre-treatment (BIP, 16 h) and the hormonal shock b...