The understanding of purple biodeterioration, or the appearance of purple tinged stains in parchment, has seen a sharp evolution in the last few years. Modern molecular analysis of the DNA, using Next-Generation Sequencing, have made it possible to hypothesize a chain of events triggered by the presence of Archaeobacteria in the parchment, a succession theory that builds on the presence of complex biological co...
Caves, with distinctive features and specialized ecosystems, harbour microorganisms that have adapted metabolically to survive in seemingly harsh conditions with limited essential elements. Despite the potential threat of biodeterioration to subterranean heritage due to microbial proliferation, caves serve as vital reservoirs of bioactive compounds produced by subsurface-adapted microorganisms. These microorgan...
The Eugénio de Almeida Foundation’s Casa de Fresco is a historical monument of valuable historic–artistic significance, which currently reveals an assortment of biofilms due to the proliferation of microorganisms in the stone and rocaille elements. The biodeterioration in this area was studied as part of the Conservation and Restoration Project. We effectively characterized the local microbial community using m...
The Alcobaça Monastery (Portugal), a UNESCO World Heritage Site, currently exhibits a high degree of surface alterations of the stone architectural elements inside the church, including an extensive pink coloration in the walls and columns, bacteria biofilms, and salt efflorescences. The main goal was to identify the microbiota that colonizes the walls and columns of this monument, to help custodians and conser...
Lanzarote (Canary Islands, Spain) is one of the best terrestrial analogs to Martian volcanology. Particularly, Lanzarote lava tubes may offer access to recognizably preserved chemical and morphological biosignatures valuable for astrobiology. By combining microbiological, mineralogical, and organic geochemistry tools, an indepth characterization of speleothems and associated microbial communities in lava tubes ...
The network of lava tubes is one of the most unexploited natural wonders of the Galapagos Islands. Here, we provide the first morphological, mineralogical, and biogeochemical assessment of speleothems fromvolcanic caves of the Galapagos to understand their structure, composition, and origin, as well as to identify organic molecules preserved in speleothems. Mineralogical analyses revealed that moonmilk and cora...
Salt caves are scarce and poorly investigated mainly because they only exist in extremely remote and arid regions, such as the Atacama Desert (Chile). The Cordillera de la Sal (San Pedro de Atacama) is one of the most important rock salt karst areas in the world forming a Mars‐like landscape. The detection of microorganisms and biosignatures preserved in halite speleothems from these hypersaline cave systems is...
Microbiome taxa associated with parasitic nematodes is unknown. These invertebrate parasites could act not only as reservoirs and vectors for horizontally transferred virulence factors, but could also provide a potential pool of future emerging pathogens. Trichuris trichiura and Trichuris suis are geohelminths parasitizing the caecum of primates, including humans, and pigs, respectively. The present work is a p...
Lava caves differ from karstic caves in their genesis and mineral composition. Subsurface microbiology of lava tube caves in Canary Islands, a volcanic archipelago in the Atlantic Ocean, is largely unknown. We have focused the investigation in a representative lava tube cave, Fuente de la Canaria Cave, in La Palma Island, Spain, which presents different types of speleothems and colored microbial mats. Four samp...
The Alcobacenses are one of the most important Portuguese collections of Monastic codices. During a campaign study to further advance our knowledge on this important legacy and its state of conservation, a heavily deteriorated codex (ALC.338) stood out due to the presence of purple stains on the lower margins on virtually all folios. In some of them, the purple stains are accompanied by yellow and brown patches...