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Microbial induced stone discoloration in alcobaça monastery: A comprehensive study

Silva, Inês; Salvador, Cátia; Miller, Ana; Dias, Luís; Candeias, António; Caldeira, Ana Teresa

The Alcobaça Monastery (Portugal), a UNESCO World Heritage Site, currently exhibits a high degree of surface alterations of the stone architectural elements inside the church, including an extensive pink coloration in the walls and columns, bacteria biofilms, and salt efflorescences. The main goal was to identify the microbiota that colonizes the walls and columns of this monument, to help custodians and conser...


The Role of Microorganisms in Pink Stone Discoloration: Insights from Batalha M...

Silva, Inês; Salvador, Cátia; Miller, Ana; Candeias, António; Caldeira, Ana Teresa

The colonization of historical buildings and monuments by fungi, algae, and bacteria is a common phenomenon. This often leads to deterioration processes that cause either visual or structural harm. The Batalha Monastery in Portugal, a UNESCOWorld Heritage Site, currently shows significant surface changes to the stone architectural elements within both the Founder’s Chapel and the church, including a widespread ...


Exploring Antimicrobial Activity of Pristine Environment Bacterial Extracts Aga...

Gatinho, Patrícia; Salvador, Cátia; Miller, Ana; Caldeira, Ana Teresa

Biodeterioration caused by biodeteriogenic microorganisms is a significant challenge for the preservation of Cultural Heritage. These microorganisms can harm important artworks, monuments, and historical sites, leading to their deterioration over time. Traditional methods to combat this issue often rely on biocides, which are chemical agents that kill or inhibit these harmful organisms. However, many biocides a...


From Biofilms to Preservation: Addressing Microbial-Induced Stone Discoloration...

Silva, Inês; Salvador, Cátia; Miller, Ana; Candeias, António; Caldeira, Ana Teresa

Throughout history, stone has been a preferred material for the construction of iconic structures due to its aesthetic appeal and intrinsic properties. However, like other construction materials, stone is subject to continuous deterioration by microorganisms, making its preservation critical for the protection of cultural heritage. Batalha Monastery (Portugal), a UNESCO World Heritage Site, exhibits notable sur...


Unveiling Microbiota within the Rocaille-Adorned Garden Grotto at Condes de Bas...

Silva, Inês; Salvador, Cátia; Arantes, Sílvia; Miller, Ana; Candeias, António; Caldeira, A. Teresa

Biodeterioration of Cultural Heritage is the consequence of interactions between living organisms, material support and environmental conditions. In stone materials, deterioration by fungi is mainly the result of mechanical, chemical, and physical processes, consisting essentially of penetration of hyphae, production of fruiting bodies, production and release of destructive extracellular organic acids, enzymes,...


Prospection of bioactive compounds produced by bacterial isolates from pristine...

Gatinho, Patrícia; Salvador, Cátia; Arantes, Sílvia; Martins, M. Rosário; Silva, Amélia; Miller, Ana; Caldeira, A. Teresa

Pristine environments can be defined as places with limited or no connections to anthropogenic activities [1], for example, karstic and marine caves, important landmarks of Natural and Cultural Heritage. Usually, these environments are exposed to extreme factors such as temperature, salinity, osmolarity, UV radiation, pressure, or pH, with values close to the limit of life. In these extreme environments, living...


Microbial induced stone discoloration in Alcobaça Monastery: a comprehensive study

Silva, Inês; Salvador, Cátia; Miller, Ana; Candeias, António; Caldeira, A. Teresa

The Alcobaça Monastery (Portugal), a UNESCO World Heritage Site, currently exhibits a high degree of surface alterations of the stone architectural elements inside the church, including an extensive pink coloration in the walls and columns (Fig. 1 A-B), bacteria biofilms, and salt efflorescences (Fig. 1C). The main goal was to identify the microbiota that colonizes the walls and columns of this monument, to hel...


Bioprospection of new compounds in pristine environments produced by bacterial ...

Gatinho, Patrícia; Salvador, Cátia; Arantes, Sílvia; M. Rosário, Martins; Miller, Ana; Caldeira, A. Teresa

Pristine environments can be defined as places with limited or no connections to anthropogenic activities [1], for example, karstic and marine caves, important landmarks of Natural and Cultural Heritage. Usually, these environments are exposed to extreme factors such as temperature, salinity, osmolarity, UV radiation, pressure, or pH, with values close to the limit of life. In these extreme environments, living...


Underneath the Purple Stain

Pinheiro, Catarina; Miller, Ana; Vaz, Patricia; Caldeira, Ana Teresa; Casanova, Conceição

The Alcobacenses are one of the most important Portuguese collections of Monastic codices. During a campaign study to further advance our knowledge on this important legacy and its state of conservation, a heavily deteriorated codex (ALC.338) stood out due to the presence of purple stains on the lower margins on virtually all folios. In some of them, the purple stains are accompanied by yellow and brown patches...


Biodeterioration of majolica glazed tiles by the fungus Devriesia imbrexigena

Coutinho, Mathilda; Miller, Ana; Phillips, Alan; Mirão, José; Dias, Luis; Rogerio-Candelera, Miguel; Saiz-Jimenez, Cesareo; Martin-Sanchez, Pedro

This study aimed at evaluating the influence of ageing on the bioreceptivity and biodeterioration of glazed tiles by fungi. These microorganisms have been identified in historical tile colonizing microbial communities. A 12-month laboratory colonization experiment was conducted using pristine and artificially aged tile models inoculated with Devriesia imbrexigena. Fungal growth was evaluated by digital image an...


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