12 documents found, page 1 of 2
Enterotoxigenic and non-enterotoxigenic Bacteroides fragilis from fecal microbi...
Ignacio,Aline; Fernandes,Miriam Rodriguez; Avila-Campos,Mario Julio; Nakano,VivianeEnterotoxigenic Bacteroides fragilis (ETBF) is an important part of the human and animal intestinal microbiota and is commonly associated with diarrhea. ETBF strains produce an enterotoxin encoded by the bft gene located in the B. fragilis pathogenicity island (BfPAI). Non-enterotoxigenic B. fragilis (NTBF) strains lack the BfPAI and usually show two different genetic patterns, II and III, based on the absence ...
Microbiota associated with chronic osteomyelitis of the jaws
Gaetti-Jardim Júnior,Elerson; Fardin,Angélica Cristiane; Gaetti-Jardim,Ellen Cristina; Castro,Alvimar Lima de; Schweitzer,Christiane MarieChronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, sa...
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian indians from U...
Vieira,Evanice Menezes Marçal; Raslan,Suzane A.; Wahasugui,Thais Cristina; Avila-Campos,Mario Julio; Marvulle,Valdecir; Gaetti-Jardim Júnior,ElersonAggregatibacter actinomycetemcomitans is associated with periodontal disease, especially localized aggressive periodontitis, produces a potent leukotoxin and its distribution is influenced by ethnic characteristics of the population. Objective: Using culture and polymerase chain reaction (PCR) techniques, this study evaluated the occurrence of this microorganism and the distribution of leukotoxic strains isolat...
Occurrence of yeasts, enterococci and other enteric bacteria in subgingival bio...
Gaetti-Jardim Júnior,Elerson; Nakano,Viviane; Wahasugui,Thais C.; Cabral,Fátima C.; Gamba,Rosa; Avila-Campos,Mario JulioThe purpose of this study was to determine the prevalence of enteric bacteria and yeasts in biofilm of 80 HIV-positive patients with plaque-associated gingivitis or necrotizing periodontitis. Patients were subjected to extra, intra oral and radiographic examinations. The oral hygiene, bleeding on probing, gingival conditions, and attachment loss were evaluated. Clinical specimens were collected from gingival cr...
Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycet...
Gaetti-Jardim Jr.,Elerson; Wahasugui,Thais Cristiane; Tomazinho,Paulo Henrique; Marques,Márcia Martins; Nakano,Viviane; Avila-Campos,Mario JulioAggregatibacter actinomycetemcomitans is an important etiologic agent of the periodontitis and is associated with extra-oral infections. In this study, the detection of the ltxA gene as well as the ltx promoter region from leukotoxic A. actinomycetemcomitans isolated from 50 Brazilian patients with periodontitis and 50 healthy subjects was performed. The leukotoxic activity on HL-60 cells was also evaluated. Le...
Detection of putative periodontal pathogens in subgingival specimens of dogs
Nishiyama,Sheila Alexandra Belini; Senhorinho,Gerusa Neyla Andrade; Gioso,Marco Antônio; Avila-Campos,Mario JulioIn this study, the presence of putative periodontal organisms, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Fusobacterium nucleatum,Dialister pneumosintes,Actinobacillus actinomycetemcomitans,Campylobacter rectus,Eikenella corrodens and Treponema denticola were examined from subgingival samples of 40 dogs of different breeds with (25) and without (15) periodontitis, by using the PCR...
Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans
Nakanishi,Fernanda Akemi; Avila-Campos,Mario Julio; Kamiji,Nádia Hizuru; Itano,Eiko NakagawaActinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. Th...
Prevotella intermedia and Porphyromonas gingivalis isolated from osseointegrate...
Pfau,Eduardo Augusto; Avila-Campos,Mario JulioThe colonization and antimicrobial susceptibility of P. intermedia and P. gingivalis isolated from peri-implant and gingival sulcus samples were determined. Samples were collected from 30 patients submitted to implant in three different times: at the moment of the surgery, 20 and 60 days after the implant installation. Organisms were identified by using a biochemical tests or API 32-A kit and by PCR. The antimi...
Analysis of the presence of pathogens which predict the risk of disease at peri...
Leitão,Joely Ângela de Oliveira; De Lorenzo,José Luiz; Avila-Campos,Mario Julio; Sendyk,Wilson RobertoThe presence of DNA of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia in the peri-implant sulcus samples of 19 partially edentulous patients was analyzed by polymerase chain reaction (PCR) and related to the depth of the peri-implant sulcus, bleeding on probing, and probable risk of disease. Ten of those patients presented a history of periodontal disease and nine of t...
Detection of pathogens from periodontal lesions
Malheiros,Veruska de João; Avila-Campos,Mario JulioOBJECTIVE: To comparatively detect A. actinomycetemcomitans and F. nucleatum from periodontal and healthy sites. METHODS: Subgingival clinical samples from 50 periodontitis adult patients and 50 healthy subjects were analyzed. Both organisms were isolated using a trypticase soy agar-bacitracin-vancomycin (TSBV) medium and detected by PCR. Conventional biochemical tests were used for bacteria identification. RES...