15 documents found, page 1 of 2
Insights into the transcriptional regulation of poorly characterized alcohol ac...
Seixas, Isabel; Santos, Diogo; Vasconcelos, Isabel; Mira, Nuno P.; Mendes-Ferreira, AnaHanseniaspora guilliermondii is a well-recognized producer of acetate esters associated with fruity and floral aromas. The molecular mechanisms underneath this production or the environmental factors modulating it remain unknown. Herein, we found that, unlike Saccharomyces cerevisiae, H. guilliermondii over-produces acetate esters and higher alcohols at low carbon-to-assimilable nitrogen (C:N) ratios, with the ...
Integrated perspective on microbe-based production of itaconic acid: from metab...
Nascimento, Miguel Figueiredo; Marques, Nuno; Correia, Joana; Faria, Nuno Torres; Mira, Nuno P.; Ferreira, Frederico CasteloABSTRACT: The discovery of itaconic acid as a product of citric acid pyrolytic distillation in 1837 opened the possibility of using it as a polymer building block. Itaconic acid, featuring two carboxylic acids and an unsaturated group, can potentially be used as a building block in several chemical syntheses, with a particular emphasis on polymer manufacture. The elucidation of biochemical pathways originating ...
Integrated perspective on microbe-based production of itaconic acid: from metab...
Nascimento, Miguel Figueiredo; Marques, Nuno; Correia, Joana; Faria, Nuno Torres; Mira, Nuno P.; Ferreira, Frederico CasteloABSTRACT: The discovery of itaconic acid as a product of citric acid pyrolytic distillation in 1837 opened the possibility of using it as a polymer building block. Itaconic acid, featuring two carboxylic acids and an unsaturated group, can potentially be used as a building block in several chemical syntheses, with a particular emphasis on polymer manufacture. The elucidation of biochemical pathways originating ...
Disclosing azole resistance mechanisms in resistant Candida glabrata strains en...
Salazar, Sara B.; Pinheiro, Maria Joana F.; Sotti-Novais, Danielle; Soares, Ana; Lopes, Maria M.; Ferreira, Teresa; Rodrigues, Vitória; Fernandes, FábioThe pathogenic yeast Candida glabrata is intrinsically resilient to azoles and rapidly acquires resistance to these antifungals, in vitro and in vivo. In most cases azole-resistant C. glabrata clinical strains encode hyperactive CgPdr1 variants, however, resistant strains encoding wild-type CgPDR1 alleles have also been isolated, although remaining to be disclosed the underlying resistance mechanism. In this st...
Transcriptomic and chemogenomic analyses unveil the essential role of Com2-regu...
Lage, Patrícia; Marques, Maria Belém Sousa Sampaio; Ludovico, Paula; Mira, Nuno P.; Ferreira, Ana MendesDuring vinification Saccharomyces cerevisiae cells are frequently exposed to high concentrations of sulfur dioxide (SO2) that is used to avoid overgrowth of unwanted bacteria or fungi present in the must. Up to now the characterization of the molecular mechanisms by which S. cerevisiae responds and tolerates SO2 was focused on the role of the sulfite efflux pump Ssu1 and investigation on the involvement of othe...
Genetic adaptive mechanisms mediating response and tolerance to acetic acid str...
Salazar, Sara; Bernardes, Rúben; Cunha, Diana; Chibana, Hiroji; Azeredo, Joana; Butler, Geraldine; Mira, Nuno P.The increased resilience of Candida glabrata to azoles and the continuous emergence of strains resistant to other antifungals demands the development of new therapeutic approaches focused on non-conventional biological targets. Genes contributing to increase C. glabrata competitiveness in the different infection sites are an interesting and unexplored cohort of therapeutic targets. To thrive in the vaginal trac...
Genetic adaptive mechanisms mediating response and tolerance to acetic acid str...
Bernardo, Ruben T.; Cunha, Diana V.; Wang, Can; Chibana, Hiroji; Silva, Sónia Carina; Sá-Correia, Isabel; Azeredo, Joana; Butler, GeraldineC. glabrata is a commensal found in the human genitourinary tract but under certain conditions this harmless colonization evolves to a mucosal infection and, in more serious cases, to disseminated mycosis. To thrive in the acidic vaginal tract C. glabrata has to cope with the presence of a competing commensal microbiota known to restrain the overgrowth of pathogens through the production of acetic and lactic ac...
Genome-wide screening of Saccharomyces cerevisiae genes required to foster tole...
Pereira, Francisco B.; Teixeira, Miguel C.; Mira, Nuno P.; Correia, Isabel Sá; Domingues, LucíliaThe presence of toxic compounds derived from biomass pre-treatment in fermentation media represents an important drawback in second-generation bio-ethanol production technology and overcoming this inhibitory effect is one of the fundamental challenges to its industrial production. The aim of this study was to systematically identify, in industrial medium and at a genomic scale, the Saccharomyces cerevisiae gene...
The CgHaa1-dependent pathway mediates Candida glabrata response and tolerance t...
Bernardo, Ruben; Silva, Sónia Carina; Cunha, Diana V.; Pereira, Leonel; Wang, Can; Correia, Isabel Sá; Chibana, Hiroji; Butler, GeraldineTo successfully colonize the vaginal tract Candida glabrata has to cope with various stresses including the presence of acetic acid at a low pH that is produced by the bacteria that co-colonize this niche. The genes/pathways involved in C. glabrata tolerance and response to acetic acid are largely unknown, although these are a highly interesting set of novel targets to control vaginal infections caused by this ...
The genome sequence of the highly acetic acid-tolerant zygosaccharomyces bailii...
Leão, Cecília; Rodrigues, Fernando José dos Santos; Santos, Júlia; Mira, Nuno P.; Münsterkötter, Martin; Valada, Filipa Dias; Palma, MargaridaIn this work, it is described the sequencing and annotation of the genome of the yeast strain ISA1307, isolated from a sparkling wine continuous production plant. This strain, formerly considered of the Zygosaccharomyces bailii species, has been used to study Z. bailii physiology, in particular, its extreme tolerance to acetic acid stress at low pH. The analysis of the genome sequence described in this work ind...