11 documents found, page 1 of 2
Bark-based biorefineries: anatomical and chemical characterization of the bark ...
Şen, Ali Umut; Simões, Rita; Yücedağ, Cengiz; Quilhó, Teresa; Sousa, Vicelina; Miranda, Isabel M.; Fernandes, Ângela; Pereira, HelenaThe detailed anatomical and chemical features of the bark from endemic Quercus vulcanica in Turkey are reported here for the first time and discussed in the perspective of integration into a bark-based biorefinery system. The bark of Q. vulcanica trees was collected and studied through observations using light and scanning electron microscopy, wet-chemical analysis, inorganic elemental and FTIR analyses, GC-MS ...
Biochemical characterization and fuel properties of endemic taurus flowering as...
Şen, Ali Umut; Simões, Rita; Yücedağ, Cengiz; Miranda, Isabel M.; Fernandes, Ângela; Pereira, HelenaTaurus flowering ash (Fraxinus ornus subsp. cilicica) is an endemic tree species in Turkey. The bark of the species was characterized for summative chemical composition, the monomeric composition of polysaccharides, phenolic content, in vitro and ex vivo antioxidant properties of hydrophilic extracts, the composition of lipophilic extractives and suberin, and thermal degradation. The bark has an elevated ash co...
The transcription factor Ndt80 is a repressor of Candida parapsilosis virulence...
Branco, Joana; Martins-Cruz, Cláudia; Rodrigues, Lisa; Silva, Raquel M.; Araújo-Gomes, Nuno; Gonçalves, Teresa; Miranda, Isabel M.; Rodrigues, Acácio G.Candida parapsilosis is an emergent opportunistic yeast among hospital settings that affects mainly neonates and immunocompromised patients. Its most remarkable virulence traits are the ability to adhere to prosthetic materials, as well as the formation of biofilm on abiotic surfaces. The Ndt80 transcription factor was identified as one of the regulators of biofilm formation by C. parapsilosis; however, its fun...
The transcription factor Ndt80 is a repressor of candida parapsilosis virulence...
Branco, Joana; Martins-Cruz, Cláudia; Rodrigues, Lisa; Silva, Raquel M.; Araújo-Gomes, Nuno; Gonçalves, Teresa; Miranda, Isabel M.; Rodrigues, Acácio G.Candida parapsilosis is an emergent opportunistic yeast among hospital settings that affects mainly neonates and immunocompromised patients. Its most remarkable virulence traits are the ability to adhere to prosthetic materials, as well as the formation of biofilm on abiotic surfaces. The Ndt80 transcription factor was identified as one of the regulators of biofilm formation by C. parapsilosis; however, its fun...
Influence of EPICardial adipose tissue in HEART diseases (EPICHEART) study: Pro...
Mancio, Jennifer; Barros, António S.; Conceicão, Glória; Santa, Cátia; Pessoa-Amorim, Guilherme; Bartosch, Carla; Fragao-Marques, MarianaAccumulation of epicardial adipose tissue (EAT) is associated with coronary artery disease (CAD) and increased risk of coronary events in asymptomatic subjects and low-risk patients, suggesting that EAT promotes atherosclerosis in its early stage. Recent studies have shown that the presence of CAD affects the properties of adjacent EAT, leading to dynamic changes in the molecular players involved in the interpl...
Antimicrobial Photodynamic Therapy against Endodontic Enterococcus faecalis and...
Diogo, Patrícia; Fernandes, Chantal; Caramelo, Francisco; Mota, Marta; Miranda, Isabel M.; Faustino, M. A. F.; Neves, M. G. P. M. S.Endodontic biofilms eradication from the infected root canal system remains as the primary focus in endodontic field. In this study, it was assessed the efficacy of antimicrobial Photodynamic Therapy (aPDT) with the Zn(II)chlorin e6 methyl ester (Zn(II)e6Me) activated by red light against monospecies and mixed biofilms of Enterococcus faecalis and Candida albicans. The results were compared with the ones obtain...
Blunted dynamics of adenosine A2A receptors is associated with increased suscep...
Rodrigues, Lisa; Miranda, Isabel M.; Andrade, Geanne M.; Mota, Marta; Cortes, Luísa; Rodrigues, Acácio G.; Cunha, Rodrigo A.; Gonçalves, TeresaOpportunistic gut infections and chronic inflammation, in particular due to overgrowth of Candida albicans present in the gut microbiota, are increasingly reported in the elder population. In aged, adult and young mice, we now compared the relative intestinal over-colonization by ingested C. albicans and their translocation to other organs, focusing on the role of adenosine A2A receptors that are a main stop si...
Development of cross-resistance by Aspergillus fumigatus to clinical azoles fol...
Faria-Ramos, Isabel; Farinha, Sofia; Neves-Maia, João; Tavares, Pedro Ribeiro; Miranda, Isabel M.; Estevinho, Leticia M.; Pina-Vaz, CidáliaBackground: The purpose of this study was to unveil whether azole antifungals used in agriculture, similar to the clinical azoles used in humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent in nature. Additionally, cross-resistance with clinical azoles was investigated. Antifungal susceptibility testing of environmental and clinical isolates of A. fumigatu...
Environmental azole fungicide, prochloraz, can induce cross-resistance to medic...
Faria-Ramos, Isabel; Tavares, Pedro Ribeiro; Farinha, Sofia; Neves-Maia, João; Miranda, Isabel M.; Silva, Raquel M.; Estevinho, Leticia M.Acquisition of azole resistance by clinically relevant yeasts in nature may result in a significant, yet undetermined, impact in human health. The main goal of this study was to assess the development of cross-resistance between agricultural and clinical azoles by Candida spp. An in vitro induction assay was performed, for a period of 90 days, with prochloraz (PCZ) - an agricultural antifungal. Afterward, the i...
Determination of chitin content in fungal cell wall: an alternative flow cytome...
Costa-de-Oliveira, Sofia; Silva, Ana P.; Miranda, Isabel M.; Salvador, Alexandre; Azevedo, Maria M.; Munro, Carol A.; Rodrigues, Acácio G.The conventional methods used to evaluate chitin content in fungi, such as biochemical assessment of glucosamine release after acid hydrolysis or epifluorescence microscopy, are low throughput, laborious, time-consuming, and cannot evaluate a large number of cells. We developed a flow cytometric assay, efficient, and fast, based on Calcofluor White staining to measure chitin content in yeast cells. A staining i...