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Comparative proteomics of Geobacter sulfurreducens PCAT in response to acetate,...

Mollaei, Monir; Timmers, Peer H. A.; Suarez-Diez, Maria; Boeren, Sjef; van Gelder, Antonie H.; Stams, Alfons Johannes Maria; Plugge, Caroline M.

Geobacter sulfurreducens is a model bacterium to study the degradation of organic compounds coupled to the reduction of Fe(III). The response of G. sulfurreducens to the electron donors acetate, formate, hydrogen and a mixture of all three with Fe(III) citrate as electron acceptor was studied using comparative physiological and proteomic approaches. Variations in the supplied electron donors resulted in differe...


Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MP...

Mollaei, Monir; Suarez-Diez, Maria; Sedano-Nunez, Vicente T.; Boeren, Sjef; Stams, Alfons Johannes Maria; Plugge, Caroline M.

We established a syntrophic coculture of Syntrophobacter fumaroxidans MPOB<sup>T</sup> (SF) and Geobacter sulfurreducens PCA<sup>T</sup> (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron a...


Syntrophic cocultures of Geobacter sulfurreducens and Syntrophobacter fumaroxid...

Mollaei, Monir; Sleutels, Tom H. J. A.; Stams, Alfons Johannes Maria; Plugge, Caroline M.

A syntrophic coculture of the electroactive bacterium Geobacter sulfurreducens and the propionate-oxidising bacterium Syntrophobacter fumaroxidans was constructed with propionate as electron donor and Fe (III) as electron acceptor. The Fe (III)-reducing G. sulfurreducens use hydrogen, formate and acetate as the electron donor, but is unable to metabolise propionate. S. fumaroxidans on the other hand cannot use ...


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