7 documents found, page 1 of 1
Single-cell liquid-core microcapsules for biomedical applications
Pires-Santos, Manuel; Carreira, Mariana; Morais, Bruno P.; Perfeito, Francisca G.; Oliveira, Mariana B.; Monteiro, Cátia F.; Nadine, Sara; Mano, João F.More recently, single-cell encapsulation emerged as a promising field in biomedicine due to its potential applications, in cell analysis and therapy. Traditional techniques involve embedding cells in crosslinked polymers to create continuous microgels, suitable mainly for adherent cells, or encapsulating them in droplets for only short-term analysis, due to their instability. In this study, we developed a metho...
Modeling 3D tumor invasiveness to modulate macrophage phenotype in a human-base...
Monteiro, Cátia F.; Almeida, Catarina R.; Custódio, Catarina A.; Mano, João F.The immune system is a pivotal player in determining tumor fate, contributing to the immunosuppressive microenvironment that supports tumor progression. Considering the emergence of biomaterials as promising platforms to mimic the tumor microenvironment, human platelet lysate (PLMA)-based hydrogel beads are proposed as 3D platforms to recapitulate the tumor milieu and recreate the synergistic tumor-macrophage c...
Tumor‐on‐a‐chip model incorporating human‐based hydrogels for easy assessment o...
Monteiro, Cátia F.; Deus, Inês A.; Silva, Inês B.; Duarte, Iola F.; Custódio, Catarina A.; Mano, João F.The coordinated migration of invasive tumor cells is a complex and dynamic mechanism driven by diverse cellular and molecular events. Unfortunately, the inherent heterogeneity within tumors raises multiple challenges in deciphering key biomarkers and novel therapeutic approaches to prevent tumor metastasis. Here, a microengineered tumor-on-a-chip system incorporating human platelet lysate hydrogels is proposed ...
Human platelet lysate-derived nanofibrils as building blocks to produce free-st...
Monteiro, Cátia F.; Gomes, Maria C.; Bharmoria, Pankaj; Freire, Mara G.; Coutinho, João A. P.; Custódio, Catarina A.; Mano, João F.Amyloid-like fibrils are garnering keen interest in biotechnology as supramolecular nanofunctional units to be used as biomimetic platforms to control cell behavior. Recent insights into fibril functionality have highlighted their importance in tissue structure, mechanical properties, and improved cell adhesion, emphasizing the need for scalable and high-kinetics fibril synthesis. In this study, we present the ...
Bioengineering a humanized 3D tri-culture osteosarcoma model to assess tumor in...
Monteiro, Cátia F.; Custódio, Catarina A; Mano, João F.To date, anticancer therapies with evidenced efficacy in preclinical models fail during clinical trials. The shortage of robust drug screening platforms that accurately predict patient's response underlie these misleading results. To provide a reliable platform for tumor drug discovery, we herein propose a relevant humanized 3D osteosarcoma (OS) model exploring the potential of methacryloyl platelet lysates (PL...
Human platelet lysates-based hydrogels: a novel personalized 3d platform for sp...
Monteiro, Cátia F.; Santos, Sara C.; Custódio, Catarina A.; Mano, João F.Fundamental physiologic and pathologic phenomena such as wound healing and cancer metastasis are typically associated with the migration of cells through adjacent extracellular matrix. In recent years, advances in biomimetic materials have supported the progress in 3D cell culture and provided biomedical tools for the development of models to study spheroid invasiveness. Despite this, the exceptional biochemica...
Three-Dimensional Osteosarcoma Models for Advancing Drug Discovery and Development
Monteiro, Cátia F.; Custódio, Catarina A.; Mano, João F.Osteosarcoma (OS) is the most common primary malignant tumor of bonethat mainly affects children and adolescents. The currently available therapiesare not effective and the search for new OS anticancer drugs is extremelyurgent. Understanding the mechanisms that underlie the tumor progression,invasion, and metastasis is an essential step toward effective cancertherapies. Tissue engineering has given a great cont...