25 documents found, page 1 of 3
Computational Insights into the Interplay of Mechanical Forces in Angiogenesis
Guerra, A; Belinha, J; Salgado, CL; Monteiro, FJ; Jorge, RNThis study employs a meshless computational model to investigate the impacts of compression and traction on angiogenesis, exploring their effects on vascular endothelial growth factor (VEGF) diffusion and subsequent capillary network formation. Three distinct initial domain geometries were defined to simulate variations in endothelial cell sprouting and VEGF release. Compression and traction were applied, and t...
Bone regeneration driven by a nano-hydroxyapatite/chitosan composite bioaerogel...
Souto Lopes, M; Liliana Grenho; Manrique, Y; Madalena M. Dias; Lopes, JCB; Fernandes MH; Monteiro, FJ; Salgado, CLThe most recent progress in reconstructive therapy for the management of periodontitis and peri-implantitis bone defects has relied on the development of highly porous biodegradable bioaerogels for guided bone regeneration. The objective of this work was to evaluate in vitro the osteoinduction of periodontal-originating cells (human dental follicle mesenchymal cells, DFMSCs) promoted by a nano-hydroxyapatite/ch...
Vancomycin-Loaded, Nanohydroxyapatite-Based Scaffold for Osteomyelitis Treatmen...
Alegrete, N; Sousa, SR; Padrão, T; Carvalho, A; Lucas, R; Canadas, RF; Lavrador, C; Alexandre, N; Gärtner, F; Monteiro, FJ; Gutierres, MThe treatment for osteomyelitis consists of surgical debridement, filling of the dead space, soft tissue coverage, and intravenous administration of antimicrobial (AM) agents for long periods. Biomaterials for local delivery of AM agents, while providing controllable antibiotic release rates and simultaneously acting as a bone scaffold, may be a valuable alternative; thus, avoiding systemic AM side effects. V-H...
Bioengineering Composite Aerogel-Based Scaffolds That Influence Porous Microstr...
Souto-Lopes,M; Fernandes, MH; Monteiro, FJ; Salgado, CLTissue regeneration of large bone defects is still a clinical challenge. Bone tissue engineering employs biomimetic strategies to produce graft composite scaffolds that resemble the bone extracellular matrix to guide and promote osteogenic differentiation of the host precursor cells. Aerogel-based bone scaffold preparation methods have been increasingly improved to overcome the difficulties in balancing the nee...
Full physicochemical and biocompatibility characterization of a supercritical C...
Souto-Lopes, M; Grenho, L; Manrique, YA; Dias, MM; Fernandes, MH; Monteiro, FJ; Salgado, CDespite bone's innate self-renewal capability, some periodontal pathologic and traumatic defects' size inhibits full spontaneous regeneration. This current research characterized a 3D porous biodegradable nano-hydroxyapatite/chitosan (nHAp/CS, 70/30) scaffold for periodontal bone regeneration, which preparation method includes the final solvent extraction and sterilization through supercritical CO2 (scCO2). Mic...
Local Antibiotic Delivery Ceramic Bone Substitutes for the Treatment of Infecte...
Alegrete, N; Sousa, SR; Peleteiro, B; Monteiro, FJ; Gutierres, MAims: the focus of this study is to evaluate if the combination of an antibiotic with a ceramic biomaterial is effective in treating osteomyelitis in an infected animal model and to define which model and protocol are best suited for in vivo experiments of local bone infection treatment. Methods: a systematic review was carried out based on PRISMA statement guidelines. A PubMed search was conducted to find orig...
A Comprehensive Look at In Vitro Angiogenesis Image Analysis Software
Pereira, M; Pinto, J; Arteaga, B; Guerra, A; Jorge, RN; Monteiro, FJ; Salgado, CLOne of the complex challenges faced presently by tissue engineering (TE) is the development of vascularized constructs that accurately mimic the extracellular matrix (ECM) of native tissue in which they are inserted to promote vessel growth and, consequently, wound healing and tissue regeneration. TE technique is characterized by several stages, starting from the choice of cell culture and the more appropriate ...
Interactions between Dental MSCs and Biomimetic Composite Scaffold during Bone ...
Costa, AC; Alves,PM; Monteiro, FJ; Salgado, CLOral–maxillofacial tumor removal can generate critical bone defects and major problems for patients, causing dysfunctionalities and affecting oral competencies such as mastication, swallowing, and breathing. The association of novel biomaterials and cell therapies in tissue engineering strategies could offer new strategies to promote osteomucosa healing. This study focused on the development of a bioengineered ...
Bioengineered fluorescent nanoprobe conjugates for tracking human bone cells: I...
Salgado, CL; Mansur, AAP; Mansur, HS; Monteiro, FJHerein, we validated novel functionalized hybrid semiconductor bioconjugates made of fluorescent quantum dots (QD) with the surface capped by chitosan (polysaccharide) and chemically modified with O-phospho-L-serine (OPS) that are biocompatible with different human cell sources. The conjugation with a directing signaling molecule (OPS) allows preferential accumulation in human bone mesenchymal stromal cells (HB...
45S5 bioglass-derived glass-ceramic scaffolds containing niobium obtained by ge...
Siqueira, L; Grenho, L; Fernandes, MH; Monteiro, FJ; Trichês, ESScaffolds of bioglass derived from BG45S5 (45 wt% SiO2, 24.5 wt% CaO, 24.5 wt% Na2O and 6 wt% P2O5) containing 10 wt% niobium were prepared by gelcasting method. The scaffolds presented a 3D porous structure with interconnected and spherical pores (pore size range 100 µm to 500 µm) and high porosity (89%), similar to trabecular architecture of spongy bone. The compressive strength was 0.18 ± 0.03 MPa which is a...