9 documents found, page 1 of 1
Photo‐compartmentalized decellularized matrix‐hyaluronan hybrid units for pancr...
Monteiro, Maria V.; Henriques-Pereira, Margarida; Neves, Bruno M.; Duarte, Iola D.; Gaspar, Vítor M.; Mano, João F.Tissue-derived decellularized extracellular matrix (dECM) provides unique biomolecular cues for bioengineering 3D tumor models with increasingly physiomimetic features. This biomimicry potential can be expanded by combining dECM with key components of native tissues, such as glycosaminoglycans (GAGs). Yet, this combination remains highly challenging, often requiring complex non-natural chemical modifications of...
Programmable living units for emulating pancreatic tumor-stroma interplay
Monteiro, Maria V.; Rocha, Marta; Gaspar, Vítor M.; Mano, João F.Bioengineering close-to-native in vitro models that emulate tumors bioarchitecture and microenvironment is highly appreciable for improving disease modeling toolboxes. Herein, pancreatic cancer living units-so termed cancer-on-a-bead models-are generated. Such user-programmable in vitro platforms exhibit biomimetic multicompartmentalization and tunable integration of cancer associated stromal elements. These st...
Advances in bioengineering pancreatic tumor-stroma physiomimetic Biomodels
Monteiro, Maria V.; Ferreira, Luís P.; Rocha, Marta; Gaspar, Vítor M.; Mano, João F.Pancreatic cancer exhibits a unique bioarchitecture and desmoplastic cancer-stoma interplay that governs disease progression, multi-resistance, and metastasis. Emulating the biological features and microenvironment heterogeneity of pancreatic cancer stroma in vitro is remarkably complex, yet highly desirable for advancing the discovery of innovative therapeutics. Diverse bioengineering approaches exploiting pat...
Advancing tissue decellularized hydrogels for engineering human organoids
Moura, Beatriz S.; Monteiro, Maria V.; Ferreira, Luís P.; Lavrador, Pedro; Gaspar, Vítor M.; Mano, João F.The extracellular matrix plays a critical role in bioinstructing cellular self-assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein we showcase the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprint...
3D-bioprinted cancer-on-a-chip: level-up organotypic in vitro models
Monteiro, Maria V.; Zhang, Yu Shrike; Gaspar, Vítor M.; Mano, João F.Combinatorial conjugation of organ-on-a-chip platforms with additive manufacturing technologies is rapidly emerging as a disruptive approach for upgrading cancer-on-a-chip systems towards anatomic-sized dynamic in vitro models. This valuable technological synergy has potential for giving rise to truly physiomimetic 3D models that better emulate tumor microenvironment elements, bioarchitecture, and response to m...
GelMA/bioactive silica nanocomposite bioinks for stem cell osteogenic different...
Tavares, Márcia T.; Gaspar, Vítor M.; Monteiro, Maria V.; Farinha, José Paulo S.; Baleizão, Carlos; Mano, João F.Leveraging 3D bioprinting for processing stem cell-laden biomaterials has unlocked a tremendous potential for fabricating living 3D constructs for bone tissue engineering. Even though several bioinks developed to date display suitable physicochemical properties for stem cell seeding and proliferation, they generally lack the nanosized minerals present in native bone bioarchitecture. To enable the bottom-up fabr...
Stratified 3D microtumors as organotypic testing platforms for screening pancre...
Monteiro, Maria V.; Gaspar, Vítor M.; Mendes, Luís; Duarte, Iola F.; Mano, João F.Cancer-associated pancreatic stellate cells installed in periacinar/periductal regions are master players in generating the characteristic biophysical shield found in pancreatic ductal adenocarcinoma (PDAC). Recreating this unique PDAC stromal architecture and its desmoplastic microenvironment in vitro is key to discover innovative treatments. However, this still remains highly challenging to realize. Herein, o...
Screening of dual chemo-photothermal cellular nanotherapies in organotypic brea...
Ferreira, Luís P.; Gaspar, Vítor M.; Monteiro, Maria V.; Freitas, Bruno; Silva, Nuno J. O.; Mano, João F.Living therapeutics approaches that exploit mesenchymal stem cells (MSCs) as nanomedicine carriers are highly attractive due to MSCs native tropism toward the 3D tumor microenvironment. However, a streamlined pre-clinical evaluation of nano-in-cell anti-cancer therapies remains limited by the lack of in vitro testing platforms for screening MSCs-3D microtumor interactions. Herein we generated dense breast cance...
Hydrogel 3D in vitro tumor models for screening cell aggregation mediated drug ...
Monteiro, Maria V.; Gaspar, Vítor M.; Ferreira, Luís P.; Mano, João F.Hydrogel-based 3D in vitro models comprising tumor ECM-mimetic biomaterials exhibit superlative potential as preclinical testing platforms for drug discovery and bioperformance screening. However, during hydrogel design and testing stages, the ideal selection between cancer cell laden 3D models or spheroid embedded hydrogel platforms remains to be elucidated. Selecting a disease-mimicking cellular arrangement w...