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Expression in Escherichia coli of cysteine protease inhibitors from cowpea (Vig...
Monteiro Júnior, José Edvar; Valadares, Napoleão Fonseca; Pereira, Humberto D’Muniz; Dyszy, Fábio Henrique; Costa Filho, Antônio José daTwo cysteine proteinase inhibitors from cowpea, VuCys1 and VuCys2, were produced in E. coli ArcticExpress (DE3). The recombinant products strongly inhibited papain and chymopapain as well as the midgut proteases from Callosobruchus maculatus larvae, a bruchid that uses cysteine proteases as major digestive enzymes. Heat treatment at 100 °C for up to 60 min or incubation at various pH values caused little reduct...
Caracterização bioquímica, funcional e estrutural de duas cistatinas recombinan...
Monteiro Júnior, José EdvarMONTEIRO JUNIOR, José Edvar. Caracterização bioquímica, funcional e estrutural de duas cistatinas recombinantes de feijão-caupi. 2012. 179 f.Tese (Doutorado em Bioquímica)-Universidade Federal do Ceará, Fortaleza-CE, 2012.; mRNA sequences coding for two cystatins were isolated from leaves of hydroponically growing cowpea plants. The sequences were ligated in the expression vector pET302NT-HIS, which was introdu...
Detecção de inibidores de proteinases cisteínicas em raízes de feijão-de-corda ...
Monteiro Júnior, José EdvarDetection of cysteine proteinase inhibitors in cowpea[Vigna unguiculata(L.) Walpers] roots as well as the accumulation of inhibitors enriched fractions throughammonium sulfate precipitation followed by reversed-phase liquid chromatography were in this present work accomplished. Fractions containing higher levels of cysteine proteinase inhibitor activity were selected and subjected to evaluation of its ability o...