45 documents found, page 1 of 5
Does a bilateral polypropylene mesh alter the duct deferens morphology, testicu...
Damous,Sérgio Henrique Bastos; Damous,Luciana Lamarão; Miranda,Jocielle dos Santos; Montero,Edna Frasson de Souza; Birolini,CláudioAbstract Purpose To evaluate the effect of a PP mesh on duct deferens morphology, testicular size and testosterone levels. Methods Forty adult male rats were distributed into groups: 1) no surgery; 2) inguinotomy; 3) mesh placed on the duct deferens; and 4) mesh placed on the spermatic funiculus. After 90 postoperative days, the inguinal region was resected, and blood samples were collected for the measurement ...
Histological analysis of the intestinal wall of newborn rats submitted to hypox...
Silvares,Soraia Guerra; Moron,Antonio Fernandes; Simões,Manuel de Jesus; Cintra,Álvaro Ulhoa; Montero,Edna Frasson de Souza; Araujo Júnior,EdwardAbstract Purpose To evaluate the effect of N-Acetylcysteine (NAC) in newborn rats submitted to hypoxia and reoxygenation (H/R) conditions in an experimental model of necrotizing enterocolitis. Methods Eight pregnant rats and their 70 cubs were used (5 groups) and exposed to H/R conditions and received NAC at different times. The animals in the H/R groups were placed in a gas chamber (100% CO2) for 10 minutes an...
Oxidative stress assessment in intestine of newborn rats submitted to hypoxia a...
Teixeira,Luciana Cristina; Fujiki,Renato Toshio Murashita; Souza,Caio Vinicius Coitinho Paiva de; Souza,Camila Mendes de; Mismetti,Maysa MoreiraAbstract Purpose: To evaluate the functional and structural response of tadalafil effects in the intestinal mucosa, using an experimental model of hypoxia and reoxygenation injury in rats. Methods: The animals were divided into 4 groups: CTL, H/R, H/R+Td and M+Td. The newborn rats allocated in groups H/R, H/R+Td and M+Td were submitted twice a day, to a gas chamber with CO2 at 100% for 10 minutes and afterward ...
Oxidative stress on ischemia/reperfusion injury in mice with non-alcoholic hepa...
Cayuela,Natalie Chaves; Negreti,Gabrielly Pascoa; Rasslan,Roberto; Koike,Marcia Kiyomi; Montero,Edna Frasson de SouzaAbstract Purpose: To evaluate the oxidative stress, resulting from ischemia and hepatic reperfusion, in mice with non-alcoholic hepatic steatosis and steatohepatitis. Methods: C57BL/6 male mice were used. Part of them were ob/ob mice, and the other part was fed with standard or MCD diets - this last used to develop steatohepatitis. The animals - MCD-I/R, ob/ob-I/R and I/R groups - were submitted to 30 minutes o...
Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic pr...
Gomes,Rúdnei de Oliveira Luciano; Artigiani Neto,Ricardo; Guimarães Neto,José de Freitas; Nunes,Adriana Porto; Montero,Edna Frasson de SouzaAbstract Purpose: To evaluate the effect of remote ischemic preconditioning (r-IPC) administered to pregnant rats, in the ileum of newborn rats subjected to hypoxia and reoxygenation. Methods: We used three pregnant rats and their newborn rats distributed in three groups: 1) Control (C) - Newborn rats born from a pregnant rat which did not undergo any intervention; 2) Hypoxia-Reoxygenation (H/R) - Newborn rats ...
Animal model of continuous peritoneal lavage with vacuum peritoneostomy
Rodrigues,Adilson Costa; Santos,Luana Carla Dos; Otsuki,Denise Aya; Saad,Karen Ruggeri; Saad,Paulo Fernandes; Montero,Edna Frasson de SouzaAbstract Purpose: To establish and evaluate the feasibility of continuous peritoneal lavage with vacuum peritoneostomy in an animal model. Methods: Eight pigs aged 3-4 months, females, were anesthetized and submitted to laparotomy and installation of a continuous peritoneal lavage with vacuum peritoneostomy. The sta-bility of the system, the physiological effects of washing with NaCl 0.9% and the sys-tem cleara...
Bacterial translocation and mortality on rat model of intestinal ischemia and o...
Costa,Rafael Izar Domingues da; Rasslan,Roberto; Koike,Marcia Kiyomi; Utiyama,Edivaldo Massazo; Montero,Edna Frasson de SouzaAbstract Purpose: To develop an experimental model of intestinal ischemia and obstruction followed by surgical resection of the damaged segment and reestablishment of intestinal transit, looking at bacterial translocation and survival. Methods: After anesthesia, Wistar rats was subject to laparotomy, intestinal ischemia and obstruction through an ileal ligature 1.5cm of ileum cecal valve; and the mesenteric ves...
Ischemic preconditioning modifies mortality and inflammatory response
Pinheiro,Daniel Faria de Campos; Fontes,Belchor; Shimazaki,John Kioshi; Heimbecker,Ana Maria Cattani; Jacysyn,Jacqueline de Fátima; Rasslan,SamirPURPOSE: To evaluate the effect of ischemic preconditioning on mortality, inflammatory mediators and oxidative stress after intestinal ischemia and reperfusion. METHODS: Male Wistar rats were allocated according to the period of ischemia with or without ischemic preconditioning which consist on clamping the superior mesenteric artery for 10 minutes followed by reperfusion for 10 minutes before the sustained isc...
Remote ischemic preconditioning and tacrolimus in the fetal small bowel transpl...
Morello,Ricardo José; Koike,Marcia Kiyomi; Abrahão,Marcos de Souza; Saad,Karen Ruggeri; Saad,Paulo Fernandes; Montero,Edna Frasson de SouzaABSTRACT PURPOSE: To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model. METHODS: Two groups were constituted: The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups: Tx, IPC-R, Fk, IPC-Fk, in both...
Effect of N-acetylcysteine in hearts of rats submitted to controlled hemorrhagi...
Oliveira Filho,Luiz Dantas de; Saad,Karen Ruggeri; Saad,Paulo Fernandes; Koike,Marcia Kiyomi; Silva,Sônia Maria da; Montero,Edna Frasson de SouzaAbstractIntroduction:Pharmacological therapy is a strategy for the prevention of complications associated with ischemia and reperfusion injury that occurs after volume replacement in the treatment of hemorrhagic shock.Objective:The aim of this study was to evaluate the effect of N-acetylcysteine associated with fluid resuscitation in cardiac injury in a rat hemorrhagic shock model.Methods:Mice Wister male rats ...