3 documents found, page 1 of 1
Alternative method for quantification of alfa-amylase activity
Farias,DF.; Carvalho,AFU.; Oliveira,CC.; Sousa,NM.; Rocha-Bezerrra,LCB.; Ferreira,PMP.; Lima,GPG.; Hissa,DC.A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured af...
Development and reproductive performance of Swiss mice in an enriched environment
Carvalho,AFU.; Araújo,AJ.; Farias,DF.; Rocha-Bezerra,LCB.; Cavalheiro,MG.The present study investigated the effects of environment enrichment on the development and reproductive performance of an outbred Swiss strain. Physical enrichment consisted of free access of animals to tubular devices of different shapes and sizes. The development evaluation was done by monitoring growth rate during 39 days, from weaning (21 days) to the onset of sexual maturity (60 days of age). Over five co...
Physiological and physico-chemical characterization of dietary fibre from the g...
Carvalho,AFU.; Portela,MCC.; Sousa,MB.; Martins,FS.; Rocha,FC.; Farias,DF.; Feitosa,JPA.This work aims to assess the potential of the green seaweed Ulva fasciata Delile as an alternative source of dietary fibre (DF). Total DF content was determined, some of its physico-chemical properties described and the physiological effects of U. fasciata meal on rats fed a hypercholesterolemic diet were investigated. U. fasciata may be considered a potential alternative source of DF with a total content of ab...