6 documents found, page 1 of 1
Engineering Zymomonas mobilis for the production of xylonic acid from sugarcane...
Herrera, Christiane Ribeiro Janner; Vieira, Vanessa Rodrigues; Benoliel, Tiago; Carneiro, Clara Vida Galvão Corrêa; De Marco, Janice LisboaSugarcane bagasse is an agricultural residue rich in xylose, which may be used as a feedstock for the production of high-value-added chemicals, such as xylonic acid, an organic acid listed as one of the top 30 value-added chemicals on a NREL report. Here, Zymomonas mobilis was engineered for the first time to produce xylonic acid from sugarcane bagasse hydrolysate. Seven coding genes for xylose dehydrogenase (X...
Produção de enzimas para diagnóstico de CoVid-19
Moraes, Lídia Maria Pepe de; Noronha, Eliane Ferreira; Torres, Fernando Araripe Gonçalves; Marco, Janice Lisboa deOs coronavírus representam um grupo de vírus cujos genomas são baseados em RNA fita simples de sentido positivo (+)ssRNA sendo causadores de diversas infecções respiratórias em humanos, incluindo a COVID-19. O diagnóstico rápido e preciso deste vírus é fundamental para nortear o tratamento da doença. Atualmente, os mais importantes testes de diagnóstico deste vírus são baseadas em imunoensaios (ELISA) ou em tes...
Multicopy plasmid integration in Komagataella phaffii mediated by a defective a...
Ocampo Betancur, Maritza; Reis, Viviane Castelo Branco; Nicola, André Moraes; De Marco, Janice Lisboa; Moraes, Lídia Maria Pepe deBackground: A commonly used approach to improve recombinant protein production is to increase the levels of expression by providing extra-copies of a heterologous gene. In Komagataella phaffii (Pichia pastoris) this is usually accomplished by transforming cells with an expression vector carrying a drug resistance marker following a screening for multicopy clones on plates with increasingly higher concentrations...
Seqüenciamento e análise dos genes das subunidades alfa e beta do hormônio folí...
Santana, Ângela Patrícia; Moraes, Lídia Maria Pepe de; Murata, Luci Sayori; Torres, Fernando Araripe GonçalvesEste trabalho relata uma clonagem e seqüenciamento das subunidades alfa e beta do hormônio folículo estimulante de Bos taurus indicus. Também apresenta os resultados de comparação realizada das seqüências gênicas dessas cadeias com as seqüências das cadeias alfa e beta do FSH de suínos e da cadeia beta de bovinos Bos taurus taurus já presentes no GenBank. Na comparação das seqüências de nucleotídeos e de aminoá...
Cloning, purification, and partial characterization of Bacillus subtilis urate ...
Pfrimer, Pollyanna; Moraes, Lídia Maria Pepe de; Galdino, Alexsandro Sobreira; Salles, Loise Pedrosa; Reis, Viviane Castelo BrancoUrate oxidase (EC 1.7.3.3) is an enzyme involved in purine metabolism which is used in the treatment of gout and as diagnostic reagent for detection of uric acid. In order to produce this enzyme in large quantities for biotechnological purposes, the gene coding for the Bacillus subtilis urate oxidase was cloned and heterologously expressed in Escherichia coli. Time course induction in E. coli showed an induced ...
Molecular epidemiology and antimicrobial susceptibility of enterococci recovere...
Almeida, Ricardo Titze de; Rollo Filho, Maurício; Silveira, Celeste Aída Nogueira; Rodrigues, Isabela Pereira; Eudes Filho, JoãoABSTRACT: We studied the antimicrobial resistance and the molecular epidemiology of 99 enterococcal surveillance isolates from two hospitals of Brasília, Brazil. Conventional biochemical tests were used to identify the enterococcal species and the disk diffusion method was used to determine their resistance profiles. Enterococcus faecalis (76%) and E. faecium (9%) were the most prevalent species. No enterococci...