9 documents found, page 1 of 1
Mitochondrial metabolism drives low-density lipoprotein-induced breast cancer c...
Nóbrega-Pereira, Sandrina; Santos, Francisco; Santos, Miguel Oliveira; Serafim, Teresa L.; Lopes, Ana Patrícia; Coutinho, Diogo; Carvalho, Filipa S.Most cancer-related deaths are due to metastases. Systemic factors, such as lipid-enriched environments [as low-density lipoprotein (LDL)-cholesterol], favor breast cancer, including triple-negative breast cancer (TNBC) metastasis formation. Mitochondria metabolism impacts TNBC invasive behavior but its involvement in a lipid-enriched setting is undisclosed. Here we show that LDL increases lipid droplets, induc...
Altered mitochondrial epigenetics associated with subchronic doxorubicin cardio...
Ferreira, André; Cunha-Oliveira, Teresa; Simões, Rui F.; Carvalho, Filipa S.; Burgeiro, Ana; Nordgren, Kendra; Wallace, Kendall B.; Oliveira, Paulo J.Doxorubicin (DOX), a potent and broad-spectrum antineoplastic agent, causes an irreversible, cumulative and dose-dependent cardiomyopathy that ultimately leads to congestive heart failure. The mechanisms responsible for DOX cardiotoxicity remain poorly understood, but seem to involve mitochondrial dysfunction on several levels. Epigenetics may explain a portion of this effect. Since mitochondrial dysfunction ma...
Cardiac cytochrome c and cardiolipin depletion during anthracycline-induced chr...
Pereira, Gonçalo C.; Pereira, Susana P.; Tavares, Ludgero C.; Carvalho, Filipa S.; Magalhães-Novais, Sílvia; Barbosa, Inês A.; Santos, Maria S.It is still unclear why anthracycline treatment results in a cardiac-specific myopathy. We investigated whether selective doxorubicin (DOX) cardiotoxicity involving mitochondrial degeneration is explained by different respiratory complexes reserves between tissues by comparing and contrasting treatment effects in heart vs liver and kidney. Alternatively, we have also explored if the degeneration is due to alter...
New derivatives of lupane triterpenoids disturb breast cancer mitochondria and ...
Serafim, Teresa L.; Carvalho, Filipa S.; Bernardo, Telma C.; Pereira, Gonçalo C.; Perkins, Edward; Holy, Jon; Krasutsky, Dmytro A.Novel cationic dimethylaminopyridine derivatives of pentacyclic triterpenes were previously described to promote mitochondrial depolarization and cell death in breast and melanoma cell lines. The objective of this work was to further investigate in detail the mechanism of mitochondrial perturbations, correlating those effects with breast cancer cell responses to those same agents. Initially, a panel of tumor an...
Doxorubicin-Induced Cardiotoxicity: From Bioenergetic Failure and Cell Death to...
Carvalho, Filipa S.; Burgeiro, Ana; Garcia, Rita; Moreno, Antonio J.; Carvalho, Rui A.; Oliveira, Paulo J.Doxorubicin (DOX) is an anticancer anthracycline that presents a dose-dependent and cumulative cardiotoxicity as one of the most serious side effects. Several hypotheses have been advanced to explain DOX cardiac side effects, which culminate in the development of life-threatening cardiomyopathy. One of the most studied mechanisms involves the activation of DOX molecule into a more reactive semiquinone by mitoch...
Dioxin-induced acute cardiac mitochondrial oxidative damage and increased activ...
Pereira, Susana P.; Pereira, Gonçalo C.; Pereira, Cláudia V.; Carvalho, Filipa S.; Cordeiro, Marília H.; Mota, Paula C.; Ramalho-Santos, J.The environmental dioxin 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is classified as a Group 1 human carcinogen and teratogenic agent.We hypothesize that TCDD-induced oxidative stress may also interfere with mitochondrial ATP-sensitive potassium channels (mitoKATP), which are known to regulate and to be regulated by mitochondrial redox state. We investigated the effects of an acute treatment of male Wistar rats...
Lipophilic Caffeic and Ferulic Acid Derivatives Presenting Cytotoxicity against...
Serafim, Teresa L.; Carvalho, Filipa S.; Marques, Maria P. M.; Calheiros, Rita; Silva, Tiago; Garrido, Jorge; Milhazes, Nuno; Borges, FernandaIn the present work, lipophilic caffeic and ferulic acid derivatives were synthesized, and their cytotoxicity on cultured breast cancer cells was compared. A total of six compounds were initially evaluated: caffeic acid (CA), hexyl caffeate (HC), caffeoylhexylamide (HCA), ferulic acid (FA), hexyl ferulate (HF), and feruloylhexylamide (HFA). Cell proliferation, cell cycle progression, and apoptotic signaling wer...
Lipophilic Caffeic and Ferulic Acid Derivatives Presenting Cytotoxicity against...
Serafim, Teresa L.; Carvalho, Filipa S.; Marques, Maria P. M.; Calheiros, Rita; Silva, Tiago; Garrido, J.M.P.J.; Milhazes, Nuno; Borges, FernandaIn the present work, lipophilic caffeic and ferulic acid derivatives were synthesized, and their cytotoxicity on cultured breast cancer cells was compared. A total of six compounds were initially evaluated: caffeic acid (CA), hexyl caffeate (HC), caffeoylhexylamide (HCA), ferulic acid (FA), hexyl ferulate (HF), and feruloylhexylamide (HFA). Cell proliferation, cell cycle progression, and apoptotic signaling wer...
Investigating drug-induced mitochondrial toxicity: a biosensor to increase drug...
Pereira, Cláudia V.; Moreira, Ana C.; Pereira, Susana P.; Machado, Nuno G.; Carvalho, Filipa S.; Sardão, Vilma A.; Oliveira, Paulo J.Mitochondria are recognized as the producers of the majority of energy cells need for their normal activity. After the initial comprehension of how mitochondrial oxidative phosphorylation produces energy, mitochondrial research was not a priority for most cell biologists until novel mitochondrial functions were identified. In fact, it is now known that mitochondria are not only involved in cell calcium homeosta...